Article
Multidisciplinary Sciences
Arnaud Landra-Willm, Ameya Karapurkar, Alexia Duveau, Anne Amandine Chassot, Lucille Esnault, Gerard Callejo, Marion Bied, Stephanie Hafner, Florian Lesage, Brigitte Wdziekonski, Anne Baron, Pascal Fossat, Laurent Marsollier, Xavier Gasull, Eric Boue-Grabot, Michael A. Kienzler, Guillaume Sandoz
Summary: By manipulating neuronal activity using optogenetics and photopharmacology, researchers have developed a light-activatable molecule called LAKI that can block pain-related potassium channels. This molecule has the potential to be a valuable tool for studying these channels, and it can also be used to remotely control pain in animal models without the need for genetic manipulations or viral infection. This non-invasive and reversible approach has applications in pain research and drug screening.
NATURE COMMUNICATIONS
(2023)
Article
Zoology
Xinsong Guo, Shan Li, Xuejie Yu, Tingting Wu, Penglai Liu, Yufeng Shao, Anan Li
Summary: The article introduces a new odor stimulation system that can precisely present odors in freely moving mice. The system can be combined with neural recordings and olfactory behavioral tests to investigate how neurons in the brain represent odor information during individual olfactory behaviors.
INTEGRATIVE ZOOLOGY
(2023)
Article
Neurosciences
Guillaume Bilodeau, Gabriel Gagnon-Turcotte, Leonard L. Gagnon, Iason Keramidis, Igor Timofeev, Yves De Koninck, Christian Ethier, Benoit Gosselin
Summary: This study presents a wireless electro-optic platform for simultaneous multimodal electrophysiological recordings and optogenetic stimulation in freely moving rodents. The platform can capture neural action potentials, local field potentials, and electromyography signals with real-time digital signal processing to reduce power consumption. It uses commercial off-the-shelf components and a low-power digital field-programmable gate array for data reduction before transmission.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Neurosciences
Connor M. McCullough, Daniel Ramirez-Gordillo, Michael Hall, Gregory L. Futia, Andrew K. Moran, Emily A. Gibson, Diego Restrepo
Summary: This study presents a head-mounted neural implant that allows for in vivo imaging of neuronal activity and simultaneous electrical recording in both head-fixed and freely moving animals. The implant design enables the recording of neural activity using optical and electrical methods, providing complementary information from each modality.
Article
Biology
Ariana R. Andrei, Samantha Debes, Mircea Chelaru, Xiaoqin Liu, Elsa Rodarte, John L. Spudich, Roger Janz, Valentin Dragoi
Summary: The study demonstrates that optogenetic inactivation of glutamatergic neurons in the superficial layers of monkey primary visual cortex induces robust suppression at the light-targeted site, but destabilizes stimulus responses in the neighboring, untargeted network. Different types of stimulus-evoked neuronal responses were identified within a cortical column, ranging from full suppression to facilitation, with mixed responses being most prominent in middle and deep cortical layers. Consistent behavioral changes induced by optogenetic inactivation were achieved when cumulative network activity was homogeneously suppressed, highlighting the importance of considering the full range of network changes outside the inactivated cortical region to avoid confounding interpretations of inactivation experiments.
Article
Engineering, Biomedical
Ori Noked, Amir Levi, Shirly Someck, Ortal Amber-Vitos, Eran Stark
Summary: The study developed a lens-free approach for constructing dual-color head-mounted, fiber-based optical units, allowing any two wavelengths to be combined. Using these devices, the same cortical PV cell could be activated and silenced, demonstrating the potential for controlling spatially intermingled neurons with distinct genetic profiles.
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2021)
Article
Physiology
Jun Kaminosono, Yuki Kambe, Akihide Tanimoto, Tomoyuki Kuwaki, Akira Yamashita
Summary: The optogenetic-based cardiac pacing method allows non-invasive stimulation of the cardiac muscle, producing blood flow and indirectly affecting respiration rhythm. This method is feasible in awake, freely moving mice and can be used to study the relationship between heartbeat state and animal behavior.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Optics
Arutyun Bagramyan, Loic Tabourin, Ali Rastqar, Narges Karimi, Frederic Bretzner, Tigran Galstian
Summary: This study introduces a novel miniature single-photon microscope with an electrically tunable liquid crystal lens for imaging in-depth fine neuronal structures in the brains of freely moving mice. The microscope is compact, lightweight, offers fast acquisition, high magnification, and high resolution, allowing for imaging of calcium activity during a wide range of behavioral tasks.
PHOTONICS RESEARCH
(2021)
Article
Chemistry, Physical
Jung-uk Lee, Wookjin Shin, Yongjun Lim, Jungsil Kim, Woon Ryoung Kim, Heehun Kim, Jae-Hyun Lee, Jinwoo Cheon
Summary: m-Torquer is a magnetic toolkit that mimics magnetoreception in nature, providing precise stimulation to cells and reliable neuromodulation in animals like mice. Its versatility allows for a wide range of applications, potentially even in large animals such as primates.
Article
Neurosciences
Kang Huang, Qin Yang, Yaning Han, Yulin Zhang, Zhiyi Wang, Liping Wang, Pengfei Wei
Summary: Measuring eye movement is important in cognitive science, and this study introduces a novel system that combines hardware and algorithms to analyze complex and dynamic eye-tracking data in freely-moving animals. This system provides a feasible approach to understanding the neural substrates of cognitive function.
NEUROSCIENCE BULLETIN
(2022)
Article
Multidisciplinary Sciences
Aghileh S. Ebrahimi, Patrycja Orlowska-Feuer, Qian Huang, Antonio G. Zippo, Franck P. Martial, Rasmus S. Petersen, Riccardo Storchi
Summary: The 3D-UPPER algorithm allows for accurate 3D reconstruction of freely moving animals, with the advantages of being fully automated, requiring no prior knowledge, and applicable to 2D data.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Guangfu Wu, Ian Heck, Nannan Zhang, Glenn Phaup, Xincheng Zhang, Yixin Wu, David E. Stalla, Zhengyan Weng, He Sun, Huijie Li, Zhe Zhang, Shinghua Ding, De-Pei Li, Yi Zhang
Summary: This study introduces a wireless, programmable push-pull microsystem for membrane-free neurochemical sampling with cellular spatial resolution in freely moving animals. It shows high efficiency in sampling various neurochemicals and captures the release of neuropeptides in freely moving mice. This system provides opportunities for understanding the modulation of diverse behavioral outputs of the brain by neuropeptide release.
Article
Biochemistry & Molecular Biology
Carlo Matera, Pablo Calve, Veronica Casado-Anguera, Rosalba Sortino, Alexandre M. J. Gomila, Estefania Moreno, Thomas Gener, Cristina Delgado-Sallent, Pau Nebot, Davide Costazza, Sara Conde-Berriozabal, Merce Masana, Jordi Hernando, Vicent Casado, M. Victoria Puig, Pau Gorostiza
Summary: Understanding the dopaminergic system is a priority, and dysregulation of dopamine receptors is associated with major neurological disorders. Current tools for studying the endogenous dopaminergic circuits are limited. This study introduces azodopa, a photoswitchable ligand that enables reversible control of dopaminergic transmission. Azodopa has shown efficacy in wild-type animals and has the potential for remote control of dopaminergic neurotransmission.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Jokubas Ausra, Mingzheng Wu, Xin Zhang, Abraham Vazquez-Guardado, Patrick Skelton, Roberto Peralta, Raudel Avila, Thomas Murickan, Chad R. Haney, Yonggang Huang, John A. Rogers, Yevgenia Kozorovitskiy, Philipp Gutruf
Summary: Wireless, battery-free, and fully subdermally implantable optogenetic tools have the potential to revolutionize neurobiological research in freely moving animals, but current devices still have issues with invasive stimulus delivery causing damage during implantation, as well as power delivery constraints limiting the size of operational arenas.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Maciej M. Jankowski, Ana Polterovich, Alex Kazakov, Johannes Niediek, Israel Nelken
Summary: Researchers developed a programmable interactive arena called the Rat Interactive Foraging Facility (RIFF) to simultaneously monitor behavior and brain activity in rats. The RIFF revealed that rats not only have auditory responses in the auditory cortex and auditory field in the posterior insula, but also show sensitivity to non-auditory parameters such as location and head-to-body angle. This study provides insights into the cognitive capabilities and learning mechanisms of rats.
Article
Behavioral Sciences
Naoto Hirasawa, Kazuyuki Yamada, Masanori Murayama
BEHAVIOURAL BRAIN RESEARCH
(2016)
Article
Multidisciplinary Sciences
D. Miyamoto, D. Hirai, C. C. A. Fung, A. Inutsuka, M. Odagawa, T. Suzuki, R. Boehringer, C. Adaikkan, C. Matsubara, N. Matsuki, T. Fukai, T. J. McHugh, A. Yamanaka, M. Murayama
Review
Neurosciences
Satoshi Manita, Hiroyoshi Miyakawa, Kazuo Kitamura, Masanori Murayama
FRONTIERS IN CELLULAR NEUROSCIENCE
(2017)
Article
Neurosciences
Daisuke Miyamoto, Daichi Hirai, Masanori Murayama
FRONTIERS IN NEURAL CIRCUITS
(2017)
Article
Neurosciences
Keisuke Ota, Yasuhiro Oisi, Takayuki Suzuki, Muneki Ikeda, Yoshiki Ito, Tsubasa Ito, Hiroyuki Uwamori, Kenta Kobayashi, Midori Kobayashi, Maya Odagawa, Chie Matsubara, Yoshinori Kuroiwa, Masaru Horikoshi, Junya Matsushita, Hiroyuki Hioki, Masamichi Ohkura, Junichi Nakai, Masafumi Oizumi, Atsushi Miyawaki, Toru Aonishi, Takahiro Ode, Masanori Murayama
Summary: By combining a resonant scanning system, large objective, photodetectors, researchers have developed a practically aberration-free, fast-scanning two-photon microscopy (FASHIO-2PM), enabling high-resolution imaging of brain regions and revealing small-world behavior of brain activities.
Article
Multidisciplinary Sciences
Daisuke Miyamoto, William Marshall, Giulio Tononi, Chiara Cirelli
Summary: The study indicates that sleep helps strengthen synapses after learning, and molecular changes in spines during sleep are related to performance after sleep. Sleep assists in enhancing the effects of learning on synapses, while also weakening remaining spines.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Akihiro Goto, Ayaka Bota, Ken Miya, Jingbo Wang, Suzune Tsukamoto, Xinzhi Jiang, Daichi Hirai, Masanori Murayama, Tomoki Matsuda, Thomas J. McHugh, Takeharu Nagai, Yasunori Hayashi
Summary: Memories are first encoded in the hippocampus and then consolidated in the cortex, with synaptic plasticity playing a crucial role. Research has shown that different phases of synaptic plasticity have different roles in memory consolidation, including local effects in the hippocampus, organizing neurons into synchronous firing assemblies during sleep, and the importance of LTP in the anterior cingulate cortex for memory stabilization.
Review
Neurosciences
Daisuke Miyamoto
Summary: This article describes the recent advances in optical imaging and manipulation approaches for studying brain dynamics during sleep. These techniques help reveal the spatiotemporal dynamics of the brain during sleep and their functional roles in awake state cognition, including learning and memory.
NEUROSCIENCE RESEARCH
(2022)
Review
Neurosciences
Daisuke Miyamoto
Summary: There is growing evidence that the brain consolidates long-term memory during sleep. Motor skill memory, a form of non-declarative procedural memory, can be enhanced through multi-sensory processing and is influenced by body movement signals from the motor brain regions. Both cortical and subcortical brain regions play a role in memory consolidation, with cortical activity being recordable and manipulable in humans and animals. During non-rapid eye movement (NREM) sleep, slow and spindle waves reflecting thalamo-cortical activity contribute to the consolidation of non-declarative memory. Animal studies have revealed dynamic cortical changes during learning and sleep, indicating the continuous reorganization of neural circuits. This article outlines the formation of sensorimotor coordination through awake learning and subsequent sleep.
NEUROSCIENCE RESEARCH
(2023)
Editorial Material
Neurosciences
Kaoru Inokuchi, Daisuke Miyamoto
NEUROSCIENCE RESEARCH
(2023)
Article
Biochemical Research Methods
Ikumi Oomoto, Hiroyuki Uwamori, Chie Matsubara, Maya Odagawa, Midori Kobayashi, Kenta Kobayashi, Keisuke Ota, Masanori Murayama
Summary: This AAV induction approach enables efficient neuronal labeling with Ca2+ sensors and allows for calcium imaging experiments throughout the entire cortical area in mice.