期刊
CHEMICO-BIOLOGICAL INTERACTIONS
卷 238, 期 -, 页码 33-39出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.cbi.2015.05.022
关键词
Sweroside; Lonicera japonica; Anti-melanogenesis; Akt; ERK; Zebrafish
资金
- Business for Cooperative R&D between Industry, Academy, and Research Institute [C0137714]
- Export Promotion Technology Development Program, Ministry of Agriculture, Food and Rural Affairs [314026-03]
In the course of screening for the melanogenesis inhibitors, sweroside was isolated from Lonicera japonica. Its chemical structure was determined on the basis of spectroscopic analysis, including mass spectroscopy and nuclear magnetic resonance analysis. Sweroside inhibited potent melanogenesis in melan-a cells at 300 mu M without cytotoxicity. Also, sweroside decreased tyrosinase, tyrosinase-related protein-1 (TRP-1) and TRP-2 protein production in melan a cells. To identify the signaling pathway of sweroside, the ability of sweroside to influence Akt and extracellular signal-regulated protein kinase (ERK) activation was investigated. Sweroside induced Akt and ERK in a dose-dependent manner. In addition, the specific inhibition of the Akt and ERK signaling pathways were studied by specific inhibitor LY294002 and U0126, respectively and it was causing the increased melanin synthesis. Furthermore, sweroside presented inhibition of the body pigmentation and tyrosinase activity in zebrafish in vivo model. These results suggest that sweroside isolated from L. japonica may be an effective skin-whitening agent through the regulates the expression of MAP kinase and melanogenic enzymes. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
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