Article
Chemistry, Multidisciplinary
Siwen Zhang, Qiyuan Chang, Pingping Li, Xiaoyu Tong, Yi Feng, Xinyao Hao, Xudong Zhang, Zhengwei Yuan, Jichun Tan
Summary: Concentrated MenSC-derived sEVs effectively repaired intrauterine adhesion in rat models, promoting gland and vascular regeneration while reversing endometrial fibrosis. Repeated MenSCs-sEVs transplantations significantly improved endometrial receptivity and pregnancy outcomes without causing hematological or biochemical abnormalities. MenSCs-sEVs safely and effectively enhanced endometrial restoration through inhibition of the TGF beta 1/SMAD3 pathway and upregulation of BMP7 expression.
Review
Biochemistry & Molecular Biology
Sevindzh Kletukhina, Guzel Mutallapova, Angelina Titova, Marina Gomzikova
Summary: Idiopathic pulmonary fibrosis (IPF) is a progressive disease caused by the aberrant regeneration of pulmonary epithelium. Mesenchymal stem cells (MSCs) and extracellular vesicles (EVs) are promising therapies for pulmonary fibrosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Ling Wang, Shiyu Liu, Ka Li, Aijin Ma, Chenghu Hu, Changlin Wang, Nan Cao, Yunpeng Zhao, Ruifeng Fu, Wenwen Jia, Peng Xiang, Houqi Liu, Zhongquan Qi, Ningwen Zhu, Lingmin Liang, Lei Wang, Jiani Cao, Peijun Zhai, Jiaxi Zhou, Jun Wei, Tao Na, Jun Wu, Zhiying He, Guangdong Zhou, Weifeng Yu, Jinyan Wu, Wen Zeng, Yong Zhang, Lijun Zhu, Boqiang Fu, Jingzhong Zhang, Shuwei Yang, Chengxiang Dai, Hengmi Cui, Jianzhong Jing, Hexin Yan, Xiaowen He, Yongbo Lu, Cailing Tong, Tongbiao Zhao, Jie Hao, Xialin Liu, Yan Jin, Yue Wang
Summary: This article introduces the general requirements for the production of extracellular vesicles derived from human stem cells in China, aiming to promote standardization in research and production in this field and accelerate international standardization.
CELL PROLIFERATION
(2023)
Article
Biochemistry & Molecular Biology
Maria Angeles de Pedro, Maria Gomez-Serrano, Federica Marinaro, Esther Lopez, Maria Pulido, Christian Preusser, Elke Pogge von Strandmann, Francisco Miguel Sanchez-Margallo, Veronica Alvarez, Javier G. Casado
Summary: The combination of interferon gamma (IFNγ) and tumor necrosis factor alpha (TNFα) was found to be the optimal priming condition for enhancing the immunomodulatory capacity of MenSCs, increasing the release of EVs and differential expressed miRNAs. MenSC-derived secretomes inhibited the proliferation of CD4+ T cells and CD8+ T cells and upregulated immune checkpoint expression in CD4+ T cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell & Tissue Engineering
Senthilkumar Alagesan, Jack Brady, Declan Byrnes, Juan Fandino, Claire Masterson, Sean McCarthy, John Laffey, Daniel O'Toole
Summary: Cell therapy, particularly mesenchymal stem/stromal (MSC) therapy, has been explored for various diseases, especially those with inflammation. However, recent studies have shown that MSC is not a cure-all. This review discusses current and future strategies to overcome limitations in MSC efficacy, including inspirations from stem cell niche and previous gene therapy work. It also explores the emerging field of extracellular vesicle therapy and enhancement protocols.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Biochemistry & Molecular Biology
Virinder Kaur Sarhadi, Ravindra Daddali, Riitta Seppanen-Kaijansinkko
Summary: Osteosarcoma is an aggressive bone tumor that predominantly affects children and adolescents, with a strong tendency to relapse and metastasize, posing challenges in identifying new therapeutic targets. Mesenchymal stem cells play a significant role in osteosarcoma development, contributing to tumor progression through intercellular signaling and communication.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell & Tissue Engineering
Yuling Huang, Lina Yang
Summary: MSC-EVs have demonstrated significant therapeutic effects on acute kidney injury, chronic kidney disease, diabetic nephropathy, and atherosclerotic renovascular disease, while their roles in transplanted kidneys remain controversial.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Cell Biology
Shuqing Zhang, Cassidy Mulder, Suzette Riddle, Rui Song, Dongmei Yue
Summary: Bronchopulmonary dysplasia (BPD) is a common complication in preterm infants, and treatment options are limited. Recent studies have shown promising results in using mesenchymal stromal/stem cells (MSCs) to treat BPD, reducing inflammation, mitochondrial impairment, lung injury, and fibrosis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Wenlu Li, Emiri T. Mandeville, Violeta Duran-Laforet, Norito Fukuda, Zhanyang Yu, Yi Zheng, Aaron Held, Ji-Hyun Park, Takafumi Nakano, Masayoshi Tanaka, Jingfei Shi, Elga Esposito, Wanting Niu, Changhong Xing, Kazuhide Hayakawa, Ignacio Lizasoain, Klaus van Leyen, Xunming Ji, Brian J. Wainger, Maria A. Moro, Eng H. Lo
Summary: The concept of the neurovascular unit highlights the importance of cell-cell signaling between neural, glial, and vascular compartments. Research has found that brain endothelial cells may release trans-differentiation signals to convert astrocytes into neural progenitor cells after stroke.
NATURE COMMUNICATIONS
(2022)
Review
Cell & Tissue Engineering
Ali Rajool Dezfuly, Azadeh Safaee, Hossein Salehi
Summary: Extracellular vesicles (EVs), including microvesicles and exosomes, carry vital information such as lipids, proteins, mRNAs, and microRNAs to target cells. Studies have shown that the therapeutic effects of stem cells are attributed to the miRNAs in their EVs, which may help in developing new therapeutic techniques for retinal degenerative diseases.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Olga Tarasiuk, Elisa Ballarini, Elisabetta Donzelli, Virginia Rodriguez-Menendez, Mario Bossi, Guido Cavaletti, Arianna Scuteri
Summary: This study investigates the interaction mechanisms between mesenchymal stem cells (MSCs) and neurons, revealing the presence of gap junctions and tunneling nanotubes between MSCs and neurons in direct co-cultures. MSCs were found to donate mitochondria to neurons and enhance neuronal survival through cell communication. Additionally, administration of extracellular vesicles derived from direct co-cultures increased neuronal survival.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Ying Luo, Dunqin Gao, Peng Wang, Cheng Lou, Tong Li, Wenhui Niu, Yingtang Gao
Summary: Extracellular vesicles derived from stem cells have significant potential in disease treatment. Optimizing culture conditions and extraction methods can yield high-quality small extracellular vesicles. The use of 0.5% ED-KSR concentration and continuous collection of culture supernatant for 5 days can maintain cell viability and pluripotent characteristics, leading to biologically active sEVs.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Review
Biochemistry & Molecular Biology
Anna Trzyna, Agnieszka Banas-Zabczyk
Summary: The secretome of ASCs, including exosomes, contains a variety of cytokines and non-coding RNAs that have therapeutic potential in a wide range of diseases. Utilizing ASC secretome as a cell-free therapy shows promise in overcoming limitations of cell-based therapies and improving treatment efficacy.
Review
Pharmacology & Pharmacy
Jafar Rezaie, Vahid Nejati, Monireh Mahmoodi, Mahdi Ahmadi
Summary: Extracellular vesicles (EVs), particularly MSCs-EVs, have shown great potential as a drug delivery system in nanomedicine. They can selectively deliver therapeutic agents to target cells and improve various diseases. However, there are still challenges that need to be addressed for the clinical translation of modified MSCs-EVs.
BIOCHEMICAL PHARMACOLOGY
(2022)
Review
Immunology
Sylwia Dabrowska, Anna Andrzejewska, Miroslaw Janowski, Barbara Lukomska
Summary: Mesenchymal stem cells (MSCs) possess supportive, differentiating, and immunoregulatory functions, while the extracellular vesicles (EVs) they produce can serve as a potential cellular therapy tool.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell & Tissue Engineering
Maroun Khoury, Patricia R. M. Rocco, Donald G. Phinney, Mauro Krampera, Ivan Martin, Sowmya Viswanathan, Jan A. Nolta, Katarina LeBlanc, Jacques Galipeau, Daniel J. Weiss
Review
Respiratory System
Maroun Khoury, Jimena Cuenca, Fernanda F. Cruz, Fernando E. Figueroa, Patricia R. M. Rocco, Daniel J. Weiss
EUROPEAN RESPIRATORY JOURNAL
(2020)
Article
Biochemistry & Molecular Biology
Rafael Contreras-Lopez, Roberto Elizondo-Vega, Maria Jose Paredes, Noymar Luque-Campos, Maria Jose Torres, Gautier Tejedor, Ana Maria Vega-Letter, Aliosha Figueroa-Valdes, Carolina Pradenas, Karina Oyarce, Christian Jorgensen, Maroun Khoury, Maria de los Angeles Garcia-Robles, Claudia Altamirano, Farida Djouad, Patricia Luz-Crawford
Article
Dentistry, Oral Surgery & Medicine
C. Brizuela, G. Meza, D. Urrejola, M. A. Quezada, G. Concha, V. Ramirez, I. Angelopoulos, M. I. Cadiz, R. Tapia-Limonchi, M. Khoury
JOURNAL OF DENTAL RESEARCH
(2020)
Article
Pharmacology & Pharmacy
Gabriela Zavala, Maria-Paz Ramos, Aliosha Figueroa-Valdes, Pablo Cisternas, Ursula Wyneken, Macarena Hernandez, Pauline Toa, Brian Salmons, John Dangerfield, Walter H. Gunzburg, Maroun Khoury
FRONTIERS IN PHARMACOLOGY
(2020)
Article
Cell Biology
Alejandro Luarte, Roberto Henzi, Anllely Fernandez, Diego Gaete, Pablo Cisternas, Matias Pizarro, Luis Federico Batiz, Isabel Villalobos, Matias Masalleras, Rodrigo Vergara, Manuel Varas-Godoy, Lorena Abarzua-Catalan, Rodrigo Herrera-Molina, Carlos Lafourcade, Ursula Wyneken
Article
Cell Biology
Carlos Andres Lafourcade, Anllely Fernandez, Juan Pablo Ramirez, Katherine Corvalan, Miguel Angel Carrasco, Andres Iturriaga, Luis Federico Batiz, Alejandro Luarte, Ursula Wyneken
Article
Dentistry, Oral Surgery & Medicine
Claudia Brizuela Cordero, Gaston Meza Santander, Denisse Urrejola Gonzalez, Andrea Quezada, Carolina Inostroza Silva, Camila Vasquez, Richard Jara, Daniel Jara, Maroun Khoury
JOURNAL OF ENDODONTICS
(2020)
Article
Multidisciplinary Sciences
R. A. Contreras-Lopez, R. Elizondo-Vega, M. J. Torres, A. M. Vega-Letter, N. Luque-Campos, M. J. Paredes-Martinez, C. Pradenas, G. Tejedor, K. Oyarce, M. Salgado, C. Jorgensen, M. Khoury, G. Kronke, M. A. Garcia-Robles, C. Altamirano, P. Luz-Crawford, F. Djouad
SCIENTIFIC REPORTS
(2020)
Article
Cell Biology
Ariel Caviedes, Barbara Maturana, Katherina Corvalan, Alexander Engler, Felipe Gordillo, Manuel Varas-Godoy, Karl-Heinz Smalla, Luis Federico Batiz, Carlos Lafourcade, Thilo Kaehne, Ursula Wyneken
Summary: This study reveals that NMDA receptor overstimulation leads to neuronal death, with NF-kappa B activity selectively enhanced in hippocampal neurons and inhibited through S-nitrosylation in resistant neurons. S-nitrosylation influences different biological processes in susceptible and resistant neurons.
CELL DEATH & DISEASE
(2021)
Article
Multidisciplinary Sciences
Luis Federico Batiz, Yasna K. Palmeiro-Silva, Gregory E. Rice, Lara J. Monteiro, Albert M. Galaburda, Roberto Romero, Mahesh A. Choolani, Ursula Wyneken, Pelusa Orellana, Sebastian E. Illanes
Summary: This study found that maternal exposure to stressful events can lead to impaired pre-reading skills in children, especially those exposed to earthquakes during the first trimester of pregnancy. Females were more impacted in alphabet knowledge, whereas males were more affected in print knowledge skills.
SCIENTIFIC REPORTS
(2021)
Review
Cell & Tissue Engineering
C. Brizuela, George T. -J. Huang, A. Diogenes, T. Botero, M. Khoury
Summary: Endodontics has made significant progress in regenerative approaches, with a clear conceptual shift in therapy. This review provides basic knowledge and practical information on stem cell-based regenerative endodontic therapy and tissue engineering procedures, as well as emerging technologies in biomaterials and signaling molecules.
STEM CELLS INTERNATIONAL
(2022)
Article
Neurosciences
Anllely Fernandez, Katherine Corvalan, Octavia Santis, Maxs Mendez-Ruette, Ariel Caviedes, Matias Pizarro, Maria -Teresa Gomez, Luis Federico Batiz, Peter Landgraf, Thilo Kahne, Alejandro Rojas-Fernandez, Ursula Wyneken
Summary: This study reveals the importance of SUMOylation in modulating the protein cargo of astrocyte-derived small extracellular vesicles (sEVs) and its potential impact on neurons.
Article
Neurosciences
Yang He, Jun Tang, Meng Zhang, Junjie Ying, Dezhi Mu
Summary: This study investigated the protective effects and mechanisms of human placenta derived mesenchymal stem cells (hPMSCs) transplantation in a rat model of hypoxic-ischemic encephalopathy (HIE). The results showed that hPMSCs transplantation reduced apoptosis and improved long-term neurological prognosis. Furthermore, the downregulation of Sema 3A/NRP-1 expression and activation of the PI3K/Akt/mTOR signaling pathway played a key role in the protective effects of hPMSCs.
Article
Neurosciences
Emily L. Isenstein, Edward G. Freedman, Jiayi Xu, Ian A. DeAndrea-Lazarus, John J. Foxe
Summary: This study evaluated electrophysiological discrimination of parametric somatosensory stimuli in healthy young adults to understand how the brain processes the duration of tactile information. The results showed that participants did not electrophysiologically discriminate between 100 and 115 ms, but they exhibited distinct electrophysiological responses when the deviant stimuli were 130, 145, and 160 ms. These findings contribute to a better understanding of tactile sensitivity in different clinical conditions.
Article
Neurosciences
Juliana R. Souza, Ludmila Lima-Silveira, Daniela Accorsi-Mendonca, Benedito H. Machado
Summary: This study demonstrates that A2A receptors play a crucial role in modulating synaptic transmission in the NTS neurons and are required for the enhancement of glutamatergic transmission observed under short-term sustained hypoxia conditions.
Article
Neurosciences
Miki Hashizume, Rina Ito, Rie Suge, Yasushi Hojo, Gen Murakami, Takayuki Murakoshi
Summary: The basolateral amygdaloid complex (BLA) is closely involved in the formation of emotional memories, including both aversive memory and contextual fear memory. Acute sleep deprivation (SD) disrupts the acquisition of tone-associated fear memory in juvenile rats, but has no significant effect on contextual fear memory. Slow network oscillation in the amygdala contributes to the formation of amygdala-dependent fear memory in relation to sleep.
Article
Neurosciences
Qunxian Wang, Shipeng Guo, Dongjie Hu, Xiangjun Dong, Zijun Meng, Yanshuang Jiang, Zijuan Feng, Weihui Zhou, Weihong Song
Summary: GSDME plays a crucial role in the pathogenesis of Alzheimer's disease by regulating the switch from apoptosis to pyroptosis and participating in neuroinflammatory response. Knockdown of GSDME has been shown to improve cognitive impairments, indicating that GSDME could be a therapeutic target for Alzheimer's disease.