Article
Cell Biology
Xiaojin Song, Bing Chen, Yiran Liang, Yaming Li, Hanwen Zhang, Dianwen Han, Yajie Wang, Fangzhou Ye, Lijuan Wang, Wenjing Zhao, Qifeng Yang
Summary: This study identified a novel circRNA, circEIF3H, with significant cancer-promoting function in the progression of TNBC. It acts as a scaffold for IGF2BP2/HuR to regulate mRNA stability. This finding provides a potential target for developing individualized therapy for TNBC.
CELL DEATH DISCOVERY
(2022)
Review
Biochemistry & Molecular Biology
Ashwin K. Chetty, Byung Hak Ha, Titus J. Boggon
Summary: This review focuses on the signaling pathways mediated by Rho family small GTPases and the specific roles of type II PAKs. The selectivity of Rho GTPases for PAK family members and the molecular basis for substrate recognition and regulation of signaling are discussed. The cross talk between Rho family small GTPases and the involvement of PAKs in diseases are also addressed.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
Vasileios L. Zogopoulos, Georgia Saxami, Apostolos Malatras, Antonia Angelopoulou, Chih-Hung Jen, William J. Duddy, Gerasimos Daras, Polydefkis Hatzopoulos, David R. Westhead, Ioannis Michalopoulos
Summary: Gene coexpression analysis aims to identify sets of genes with similar expression patterns across multiple data sets, such as microarray experiments. The Arabidopsis Coexpression Tool (ACT) generates a coexpression tree from processed microarray data, revealing relationships between different genes. ACT offers a user-friendly interface for gene set enrichment analysis and is successful in identifying both ubiquitous and tissue-specific gene expressions.
Review
Oncology
Xiaodong Li, Feng Li
Summary: Gastrointestinal tumors are the most common tumors with high mortality rate worldwide. PAK, a serine/threonine kinase, plays a crucial role in tumor phenotype by influencing cell proliferation, survival, motility, tumor cell plasticity, and the tumor microenvironment. Understanding the features and signaling pathways of PAK in cells, especially its role in gastrointestinal tumors, can provide a theoretical basis for future research on PAK and potential therapeutic targets.
Article
Oncology
Yi Ma, Mehrdad Nikfarjam, Hong He
Summary: Pancreatic Ductal Adenocarcinoma (PDA) is a deadly cancer with KRAS mutation as a common oncogene. P21-activated kinases (PAKs) are downstream mediators of KRAS and play a role in PDA tumorigenesis. Inhibiting PAK reduces cancer cell viability and sensitizes them to chemotherapy. PAK inhibition also increases cancer immunogenicity in melanoma, glioblastoma, and PDA. Autophagy is important in PDA immune evasion and there is a connection between PAK and cancer cell autophagy.
Article
Engineering, Environmental
Jose de Jesus Montoya-Rosales, Aura Ontiveros-Valencia, Diego A. Esquivel-Hernandez, Claudia Etchebehere, Lourdes B. Celis, Elias Razo-Flores
Summary: Microbial communities in dark fermentation continuous systems are influenced by substrate type, concentration, and product accumulation. This study used metatranscriptomics and quantitative PCR to investigate the effect of high organic loading rates on microbial community diversity and expression of key dark fermentative genes. The results showed that the microbial communities consisted of H-2-producing bacteria, homoacetogens, and lactic acid bacteria. Increasing the organic loading rate influenced the abundance of genes and gene transcripts related to H-2 production and homoacetogenesis. Adding silicone oil increased H-2 production and the abundance of related genes and gene transcripts.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ifeoluwapo Matthew Joshua, Meng Lin, Ariestia Mardjuki, Alessandra Mazzola, Thomas Hoefken
Summary: The p21-activated kinases (PAKs) are important signaling proteins that contribute to various cellular processes and human diseases. By studying the yeast PAK Ste20, researchers identified 56 proteins, mostly undocumented, that interact with Ste20. Ste20 plays a significant role in glucose metabolism and gene expression and induces filamentous growth through interaction with nuclear proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Leon J. Blankenstein, Nils Cordes, Leoni A. Kunz-Schughart, Anne Vehlow
Summary: In this study, PAK4 was identified as the main regulator of invasion and radio-chemotherapy resistance in glioblastoma cells. By enhancing the number of DNA double-strand breaks and reducing the expression of DNA repair proteins, PAK4 inhibition could improve the radiosensitivity and impair DNA repair in glioblastoma.
Article
Biochemistry & Molecular Biology
Ann-Kathrin Schmidt, Karoline Pudelko, Jan-Eric Boekenkamp, Katharina Berger, Maik Kschischo, Holger Bastians
Summary: Whole chromosome instability is a hallmark of human cancer and can be induced by abnormal microtubule assembly rates during mitosis, triggering errors in chromosome segregation. Loss of tumor suppressor genes can lead to increased mitotic microtubule assembly rates and contribute to chromosome instability in cancer cells.
Article
Multidisciplinary Sciences
Soudeh Ghafouri-Fard, Arash Safarzadeh, Mohammad Taheri, Elena Jamali
Summary: A co-expression network of differentially expressed genes (DEGs) related to colorectal cancer (CRC) and their target genes was constructed using the weighted gene co-expression network analysis (WGCNA) algorithm. GO and KEGG pathway analysis revealed that these genes were mainly involved in the regulation of hormone levels, extracellular matrix organization, and extracellular structure organization. The study also identified DKC1, PA2G4, LYAR and NOLC1 as the key hub genes of CRC.
SCIENTIFIC REPORTS
(2023)
Article
Cell Biology
Jeeho Kim, Ra Young Park, Younghoon Kee, Sunjoo Jeong, Takbum Ohn
Summary: Serine/arginine-rich splicing factor 3 (SRSF3) is known to regulate gene expression at the splicing level, but it also has a splicing-independent role. This study shows that SRSF3 interacts with eIF4A1 to control the translation of p21 mRNA, providing a new mode of regulation for p21 expression at the translational level.
CELL DEATH & DISEASE
(2022)
Article
Oncology
Stefania Belli, Daniela Esposito, Alessandra Allotta, Alberto Servetto, Paola Ciciola, Ada Pesapane, Claudia M. Ascione, Fabiana Napolitano, Concetta Di Mauro, Elena Vigliar, Antonino Iaccarino, Carmine De Angelis, Roberto Bianco, Luigi Formisano
Summary: Resistance to ER antagonists in combination with CDK4/6 inhibitors in ER+ breast cancers is associated with up-regulation of MAPK and EMT pathways, with Pak1 playing a pivotal role. Inhibiting Pak1 could be a promising strategy to overcome resistance in these patients.
Article
Environmental Sciences
Mary Jean See, David C. Bencic, Robert W. Flick, Jim Lazorchak, Adam D. Biales
Summary: This study characterized the VTG protein in male fathead minnow mucus compared to plasma and liver mRNA. The results showed that the induction kinetics of VTG protein concentration in mucus and plasma were similar, with a significant increase detected after 2 days of exposure in the mucus-based assay and after 7 days in the plasma-based assay.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Review
Chemistry, Medicinal
Hanxun Wang, Peilu Song, Yinli Gao, Lanlan Shen, Hanqin Xu, Jian Wang, Maosheng Cheng
Summary: This review summarized the development of PAK4 inhibitors reported in patents over the past two decades, classified into different types with detailed elucidation of design ideas and structure-activity relationships. Studies have shown that there are challenges in the clinical and preclinical research of PAK4 inhibitors currently, but the combination with immunotherapy may provide a new direction for development.
EXPERT OPINION ON THERAPEUTIC PATENTS
(2021)
Article
Biology
Eunjeong Jung, Rebecca Capel, Congshan Jiang, Elisa Venturi, Georgiana Neagu, Sarah Pearcey, Yafei Zhou, Yanmin Zhang, Ming Lei
Summary: Pak1 deficiency in the heart leads to atrial arrhythmias, including atrial fibrillation, under adrenergic stress. This is not due to interstitial fibrosis, but rather to alterations in the expression and phosphorylation of key molecules involved in calcium homeostasis.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Anabel Alvarez Julia, Alberto C. Frasch, Beata Fuchsova
JOURNAL OF NEUROCHEMISTRY
(2016)
Article
Neurosciences
Karina Formoso, Micaela D. Garcia, Alberto C. Frasch, Camila Scorticati
MOLECULAR AND CELLULAR NEUROSCIENCE
(2016)
Article
Neurosciences
Micaela D. Garcia, Karina Formoso, Gabriela I. Aparicio, Alberto C. C. Frasch, Camila Scorticati
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2017)
Article
Biochemistry & Molecular Biology
Alejandra P. Frasch, Leon A. Bouvier, Florencia M. Oppenheimer, Maria Aparecida Juliano, Luiz Juliano, Adriana K. Carmona, Juan Jose Cazzulo, Gabriela T. Niemirowicz
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
(2018)
Article
Microbiology
Maria Albertina Romaniuk, Alberto Carlos Frasch, Alejandro Cassola
Article
Chemistry, Medicinal
Zexin Chen, Patricia Marce, Ricardo Resende, Pedro M. Alzari, A. Carlos Frasch, Jean M. H. van den Elsen, Susan J. Crennell, Andrew G. Watts
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2018)
Article
Multidisciplinary Sciences
Melisa C. Monteleone, Silvia C. Billi, Marcela A. Brocco, Alberto C. Frasch
SCIENTIFIC REPORTS
(2017)
Article
Neurosciences
Nicolas M. Rosas, Anabel Alvarez Julia, Sofia E. Alzuri, Alberto C. Frasch, Beata Fuchsova
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2018)
Article
Biochemistry & Molecular Biology
Sofia Elisa Alzuri, Nicolas Matias Rosas, Natasa Hlavacova, Daniela Jezova, Beata Fuchsova
Summary: The neuronal membrane glycoprotein M6a (GPM6A) plays a role in neuronal remodeling and plasticity. Chronic stress can regulate the expression of Gpm6a through miR-124-mediated impact on Hdac5 and Mef2c. BDNF can activate Gpm6a expression.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Medicine, General & Internal
Kinga Szigeti, Ivanna Ihnatovych, Nicolas Rosas, Ryu P. Dorn, Emily Notari, Eduardo Cortes Gomez, Muye He, Ivan Maly, Shreyas Prasad, Erik Nimmer, Yuna Heo, Beata Fuchsova, David A. Bennett, Wilma A. Hofmann, Arnd Pralle, Yongho Bae, Jianmin Wang
Summary: This study investigates the role of CHRFAM7A in the human brain using a multiomics approach and reveals that CHRFAM7A modulates intracellular calcium dynamics and regulates Rac1 activation, leading to changes in the actin cytoskeleton and increased stiffness resistance. This finding contributes to the understanding of CHRFAM7A's role in the human brain.
Article
Neurosciences
Gabriela I. Aparicio, Karina Formoso, Antonella Leon, Alberto C. Frasch, Camila Scorticati
FRONTIERS IN SYNAPTIC NEUROSCIENCE
(2020)
Article
Neurosciences
Yang He, Jun Tang, Meng Zhang, Junjie Ying, Dezhi Mu
Summary: This study investigated the protective effects and mechanisms of human placenta derived mesenchymal stem cells (hPMSCs) transplantation in a rat model of hypoxic-ischemic encephalopathy (HIE). The results showed that hPMSCs transplantation reduced apoptosis and improved long-term neurological prognosis. Furthermore, the downregulation of Sema 3A/NRP-1 expression and activation of the PI3K/Akt/mTOR signaling pathway played a key role in the protective effects of hPMSCs.
Article
Neurosciences
Emily L. Isenstein, Edward G. Freedman, Jiayi Xu, Ian A. DeAndrea-Lazarus, John J. Foxe
Summary: This study evaluated electrophysiological discrimination of parametric somatosensory stimuli in healthy young adults to understand how the brain processes the duration of tactile information. The results showed that participants did not electrophysiologically discriminate between 100 and 115 ms, but they exhibited distinct electrophysiological responses when the deviant stimuli were 130, 145, and 160 ms. These findings contribute to a better understanding of tactile sensitivity in different clinical conditions.
Article
Neurosciences
Juliana R. Souza, Ludmila Lima-Silveira, Daniela Accorsi-Mendonca, Benedito H. Machado
Summary: This study demonstrates that A2A receptors play a crucial role in modulating synaptic transmission in the NTS neurons and are required for the enhancement of glutamatergic transmission observed under short-term sustained hypoxia conditions.
Article
Neurosciences
Miki Hashizume, Rina Ito, Rie Suge, Yasushi Hojo, Gen Murakami, Takayuki Murakoshi
Summary: The basolateral amygdaloid complex (BLA) is closely involved in the formation of emotional memories, including both aversive memory and contextual fear memory. Acute sleep deprivation (SD) disrupts the acquisition of tone-associated fear memory in juvenile rats, but has no significant effect on contextual fear memory. Slow network oscillation in the amygdala contributes to the formation of amygdala-dependent fear memory in relation to sleep.
Article
Neurosciences
Qunxian Wang, Shipeng Guo, Dongjie Hu, Xiangjun Dong, Zijun Meng, Yanshuang Jiang, Zijuan Feng, Weihui Zhou, Weihong Song
Summary: GSDME plays a crucial role in the pathogenesis of Alzheimer's disease by regulating the switch from apoptosis to pyroptosis and participating in neuroinflammatory response. Knockdown of GSDME has been shown to improve cognitive impairments, indicating that GSDME could be a therapeutic target for Alzheimer's disease.