Article
Endocrinology & Metabolism
Marius M. Mader, Rainer Boeger, Daniel Appel, Edzard Schwedhelm, Munif Haddad, Malte Mohme, Katrin Lamszus, Manfred Westphal, Patrick Czorlich, Juliane Hannemann
Summary: This study revealed significant alterations in L-arginine metabolism after hemorrhagic stroke, including changes in key substrates and products in both plasma and cerebrospinal fluid, as well as the activity of enzymes like NOS and arginase. Early reduction in CSF L-arginine concentration was identified as an independent risk factor for poor outcome.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2021)
Article
Biochemistry & Molecular Biology
Iwona Bednarz-Misa, Mariusz G. Fleszar, Paulina Fortuna, Lukasz Lewandowski, Magdalena Mierzchala-Pasierb, Dorota Diakowska, Malgorzata Krzystek-Korpacka
Summary: The study identified aberrations in L-arginine metabolism in gastric cancer, reflecting different subtypes and pathology. A metabolite diagnostic panel showed high accuracy in detecting and differentiating gastric cancer from benign disorders, while enzyme gene expression was related to tumor advancement and metastasis. Further functional studies on ASL, PRMT2, and ORNT1 in gastric cancer are warranted.
Article
Multidisciplinary Sciences
Patrycja Molek, Pawel Zmudzki, Aleksandra Wlodarczyk, Jadwiga Nessler, Jaroslaw Zalewski
Summary: In patients with ST-segment elevation myocardial infarction, arginine metabolism shifted from nitric oxide synthase towards arginase during ischemia. Lower arginine concentrations at 6-month follow-up were associated with worse long-term outcomes.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Juliane Hannemann, Debra J. Skene, Benita Middleton, Edzard Schwedhelm, Anika Laing, Rainer Boeger
Summary: Asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) interfere with nitric oxide (NO) formation from L-arginine via different mechanisms. The plasma concentrations of L-arginine-related amino acid metabolites show diurnal variation, with L-arginine and homoarginine concentrations having a 12-hour period. ADMA and SDMA show a 24-hour rhythmicity. Night shift work does not significantly affect the central suprachiasmatic nuclei clock timing, melatonin and cortisol levels, or blood pressure patterns.
Review
Cell Biology
Marius Marc-Daniel Mader, Patrick Czorlich
Summary: This review discusses the role of L-arginine metabolism in patients with acute and critical neurological disorders, highlighting its importance in neuro-intensive care treatment.
NEURAL REGENERATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yukiko K. Kaneko, Ami Morioka, Misaki Sano, Maho Tashiro, Naoya Watanabe, Nahoko Kasahara, Masato Nojiri, Chihiro Ishiwatari, Kentaro Ichinose, Akira Minami, Takashi Suzuki, Momoka Yamaguchi, Toshihide Kimura, Tomohisa Ishikawa
Summary: This study found that high concentrations of glucose and the insulin receptor blocker S661 reduced the expression of DDAH2 in pancreatic β cells, leading to the accumulation of ADMA. The accumulation of ADMA suppressed the production of nitric oxide, resulting in increased apoptosis of pancreatic β cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Environmental Sciences
Ahmed A. Sedik, Azza Hassan, Dalia O. Saleh
Summary: The aim of this study was to investigate the impact of arginine (ARG) on thioacetamide (TAA)-induced hepatic encephalopathy (HE) in rats. The results showed that ARG restored serum and brain ammonia levels, improved liver function, behavioral alterations, and neuro-biochemical values. ARG also reduced inflammation and oxidative stress and improved immunological reactivity in the cerebellum and liver tissues. Overall, ARG played a beneficial role in modulating the adverse complications associated with TAA-induced HE in rats.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Ophthalmology
Tadanobu Yoshikawa, Kenji Obayashi, Kimie Miyata, Keigo Saeki, Nahoko Ogata
Summary: The study revealed a significant association between higher serum ADMA levels and severe glaucoma, which remained significant after adjusting for potential confounders.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Oncology
Linfeng Chen, Qiming Tang, Keying Zhang, Qianyang Huang, Yun Ding, Bo Jin, Szumam Liu, Kuoyuan Hwa, C. James Chou, Yani Zhang, Sheeno Thyparambil, Weili Liao, Zhi Han, Richard Mortensen, James Schilling, Zhen Li, Robert Heaton, Lu Tian, Harvey J. Cohen, Karl G. Sylvester, Rebecca C. Arent, Xinyang Zhao, Doff B. Mcelhinney, Yumei Wu, Wenpei Bai, Xuefeng B. Ling
Summary: This study explores the potential use of metabolic changes as biomarkers for assessing ovarian cancer. The research identifies key gene expression pathways and proposes symmetric dimethylarginine (SDMA) and arginine as potential liquid biopsy biomarkers for ovarian cancer assessment.
Article
Cell Biology
Garrett A. Clemons, Alexandre Couto E. Silva, Christina H. Acosta, Mariana Sayuri Berto Udo, Vesna Tesic, Krista M. Rodgers, Celeste Yin-Chieh Wu, Cristiane T. Citadin, Reggie Hui-Chao Lee, Jake T. Neumann, Shailaja Allani, Howard Prentice, Quanguang Zhang, Hung Wen Lin
Summary: Alzheimer's disease is a major cause of mortality, disability, and long-term care burden, primarily affecting women. This study explores the role of PRMT4 and its downstream product, ADMA, in the dysregulation of NOS and cerebral blood flow in AD. The findings suggest the potential importance of PRMT4 signaling in the pathogenesis of Alzheimer's-related cerebrovascular derangement.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Volkan Sengul, Zeliha Guney, Sivge Kurgan, Canan Onder, Muhittin A. Serdar, Meral Gunhan
Summary: This study investigated the levels of NOS, methylated arginine metabolites, and IL-6 in serum and saliva of patients with advanced periodontal diseases. The results showed a significant relationship between clinical parameters, especially ADMA levels in saliva, and periodontal inflammation.
CLINICAL ORAL INVESTIGATIONS
(2022)
Article
Multidisciplinary Sciences
Dain (Raina) Kim, Sarah Martin, Kaushik Desai
Summary: Oral L-arginine supplements have nitric oxide mediated vasodilation effects, but their physiological impact is not fully understood. This study investigated the effects of oral L-arginine and D-arginine at a higher dose and longer treatment duration on the expression and activity of arginine metabolizing enzymes and levels of their metabolites in rats. The results showed that L-arginine and D-arginine significantly altered various enzymes and metabolites in the arginine metabolic pathways, indicating the need for further studies to determine the safe and effective dosage and duration of oral L-arginine use.
Article
Chemistry, Analytical
Adela Cortes Gimenez-Coral, Pablo Rodriguez-Gonzalez, Adriana Gonzalez Gago, Eva Cernuda Morollon, Elena Lopez-Cancio, Belen Prieto Garcia, J. Ignacio Garcia Alonso
Summary: This study evaluates the use of one- and two-dimensional liquid chromatography for quantifying three stroke outcome predictors in plasma. The isotopically labelled analogues of l-arginine, asymmetric dimethylarginine, and symmetric dimethylarginine are utilized for the quantification. The results show that one-dimensional liquid chromatography, combined with a derivatization step, provides satisfactory results for quantification.
Article
Medical Laboratory Technology
Lavinia Santucci, Sara Lomuscio, Aniello Primiano, Riccardo Calvani, Silvia Persichilli, Federica Iavarone, Anna Picca, Francesca Canu, Andrea Urbani, Jacopo Gervasoni
Summary: This study aimed to develop a rapid method for the simultaneous determination of arginine and its metabolites in human plasma. A simple deproteinization procedure and hydrophilic interaction liquid chromatography were used for the pre-analytical and chromatographic separation, respectively. The method showed good recovery, precision, and stability, making it suitable for both research and clinical routine use.
CLINICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Maciej Bladowski, Ewa Szahidewicz-Krupska, Jerzy Wisniewski, Paulina Fortuna, Justyna Chojdak-Lukasiewicz, Slawomir Budrewicz, Mariusz Fleszar, Adrian Doroszko
Summary: This study aimed to analyze the differences in the expression of the nitric oxide metabolic pathway in platelets and plasma between ischemic stroke patients and non-stroke individuals. It also investigated the relationship between these parameters and platelet aggregation. The results showed that stroke patients had higher levels of NOS inhibitors in platelets, and stroke management increased the bioavailability of nitric oxide in platelets.
Review
Medicine, General & Internal
Anna Paulina Domaszewska-Szostek, Marta Olga Krzyzanowska, Anna Maria Czarnecka, Maria Siemionow
Summary: This review summarizes the current state of knowledge on local treatment of burn wounds, focusing on the use of cellular therapies in clinical studies, particularly on keratinocytes and fibroblasts. Promising data demonstrate the effectiveness of various cell-based therapies, but further evidence is required to demonstrate their clinical efficacy and safety in burns.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Neurosciences
Marek J. Pawlik, Marta Obara-Michlewska, Mariusz P. Popek, Anna Maria Czarnecka, Stanislaw J. Czuczwar, Jarogniew Luszczki, Marcin Kolodziej, Albert Acewicz, Teresa Wierzba-Bobrowicz, Jan Albrecht
Summary: Pretreatment with GS inhibitor MSO did not significantly affect hippocampal GS activity but reduced the number of animals showing convulsive seizures and delayed the onset of electrographic seizures. The study suggests that MSO impairs a specific aspect of glutamatergic transmission involved in the transition from cholinergic stimulus to seizure onset, while leaving the nature of the Glu pool(s) involved in altered transmission undefined.
Article
Biochemistry & Molecular Biology
El Hassan Hamdani, Mariusz Popek, Malgorzata Frontczak-Baniewicz, Tor Paaske Utheim, Jan Albrecht, Magdalena Zielinska, Farrukh Abbas Chaudhry
Summary: The study investigates the impact of ammonia on Slc38 transporters, including competition with glutamine binding and effects on intracellular pH, membrane potential, and K+ buffering; Knocking out the Slc38a3 gene in mice results in cerebral cortical edema and disrupted neurotransmitter synthesis; A mouse model of acute liver failure shows downregulation of Slc38a3 protein, inhibited astroglial glutamine release, and cytotoxic edema.
Article
Biochemistry & Molecular Biology
Krzysztof Milewski, Anna Maria Czarnecka, Jan Albrecht, Magdalena Zielinska
Summary: Acute liver failure leads to deregulated nitric oxide signaling in the brain, resulting in reduced NOS activity and cofactor BH4 content, as well as eNOS uncoupling. These changes are associated with increased reactive oxidative species and decreased cerebral cortical blood flow, indicating a potential causal relationship between eNOS dysfunction and neurologic complications of ALF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Mariusz Popek, Bartosz Bobula, Karolina Orzel-Gajowik, Magdalena Zielinska
Summary: The study suggests that decreased platelet count in patients with acute liver failure may lead to a decrease in TGF-beta 1 levels and affect the levels of synaptic proteins in the brain, potentially impairing the docking of synaptic vesicles to the postsynaptic membrane. However, these alterations do not seem to impact neurotransmission and may not contribute to neurological deficits in patients with acute liver failure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Katarzyna Dabrowska, Katarzyna Skowronska, Mariusz Popek, Jan Albrecht, Magdalena Zielinska
Summary: The study found that dysregulation of the SN1 transporter by ammonia in astrocytes involves activation of Nrf2, but does not require the formation of the Sp1-Nrf2 complex.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Karolina Orzel-Gajowik, Krzysztof Milewski, Magdalena Zielinska
Summary: Acute liver failure (ALF) is a life-threatening condition characterized by rapid loss of liver function. ALF can cause a range of neuropsychiatric symptoms called acute hepatic encephalopathy. Micro RNAs (miRNAs), small non-coding RNAs that regulate gene expression, have been identified as potential biomarkers for various diseases. In a systematic literature review of 852 ALF patients, 205 altered circulating miRNAs were identified, with 25 miRNAs consistently altered in the blood. These 25 miRNAs, primarily found in patients with acetaminophen overdose, show promise as potential biomarkers for diagnosing symptomatic ALF. The selected miRNAs are believed to play a role in blood-brain barrier function. Further research is needed to establish a causal relationship between differential expression of circulating miRNAs and specific clinical features of ALF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Magdalena Zielinska, Tomasz Hermanowski
Summary: The study revealed that primary care physicians in Poland often rely on medical representatives, medical journals, and conferences for information on medicinal products. Despite considering clinical guidelines as the most credible source with the greatest impact on their prescribing decisions, physicians do not utilize them as frequently as other sources. More strategies and tools are needed to provide clinicians with reliable information sources.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Krzysztof Milewski, Karolina Orzel-Gajowik, Magdalena Zielinska
Summary: The mechanisms underlying cerebral vascular dysfunction and edema during hepatic encephalopathy (HE) are unclear. This review focuses on the possible involvement of mitochondrial dysfunction in the status of brain endothelial cell mitochondria in HE.
NEUROCHEMICAL RESEARCH
(2022)
Review
Neurosciences
Magdalena Zielinska, Jan Albrecht, Mariusz Popek
Summary: Acute liver failure causes hyperammonemia and increased ammonia accumulation in the brain. This is associated with increased brain glutamine content. This review focuses on the effects of hyperammonemia on glutamine carriers in astrocytes and their contribution to brain edema.
FRONTIERS IN NEUROSCIENCE
(2022)
Correction
Biochemistry & Molecular Biology
Krzysztof Milewski, Karolina Orzel-Gajowik, Magdalena Zielinska
NEUROCHEMICAL RESEARCH
(2022)
Article
Clinical Neurology
Marek Pawlik, Anna Maria Czarnecka, Marcin Kolodziej, Katarzyna Skowronska, Michal Wegrzynowicz, Martyna Podgajna, Stanislaw Jerzy Czuczwar, Jan Albrecht
Summary: This study found that pretreatment with a glutamine synthetase inhibitor, methionine sulfoximine (MSO), can mitigate the intensity of seizures during the initial phase. The protective effect of MSO is achieved by preventing an increase in cell volume.
Article
Medicine, General & Internal
Anna Maria Czarnecka, Marta Obara-Michlewska, Dorota Wesol-Kucharska, Milena Greczan, Magdalena Kaczor, Janusz Ksiazyk, Dariusz Rokicki, Magdalena Zielinska
Summary: From the results, we found that the plasma level of S100B is positively correlated with ammonia level, while the plasma level of 3-NT does not correlate with ammonia concentration. This offers a unique perspective for future identification and monitoring of neurological deficits risk-linked with hyperammonemia episodes in patients with inherited hyperammonemias.
JOURNAL OF CLINICAL MEDICINE
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
Marek Pawlik, Anna Maria Czarnecka, Katarzyna Skowronska, Marcin Kolodziej, Krystyna Golembiowska, Jan Albrecht
JOURNAL OF NEUROCHEMISTRY
(2022)