Review
Cell Biology
Ronald B. Driesen, Pascal Gervois, Tim Vangansewinkel, Ivo Lambrichts
Summary: The apical papilla, a stem cell-rich tissue at the base of developing dental root, plays a crucial role in root elongation and maturation. Multipotent stem cells of the apical papilla (SCAP) show high potential for differentiation and are considered as a promising source for stem cell-based therapies. Further research on the specific signaling pathways, heterogeneity, and microenvironmental interaction of SCAP within the tissue could potentially lead to novel strategies in dental root regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Polymer Science
Mohammad Foad Abazari, Shohreh Zare Karizi, Nazanin Hajati-Birgani, Mina Kohandani, Sepehr Torabinejad, Fatemeh Nejati, Navid Nasiri, Mohammad Hossein Maleki, Hassan Mohajerani, Vahid Mansouri
Summary: The study focused on fabricating poly(3-hydroxybutyrate)/poly(l-lactic acid) nanofibers and curcumin-incorporated nanofibers using electrospinning, and evaluating their potential for osteogenic differentiation of human adipose-derived stem cells. Results showed that curcumin-incorporated nanofibers had better biological properties and could support osteogenic differentiation more efficiently compared to empty nanofibers, indicating their promising potential for bone tissue engineering.
POLYMERS FOR ADVANCED TECHNOLOGIES
(2021)
Article
Nanoscience & Nanotechnology
Yuan Dai, Weizhong Wang, Xiaojun Zhou, Linli li, Yuyi Tang, Minghao Shao, Feizhou Lyu
Summary: The transplantation of human neural stem cells (hNSCs) combined with bioactive nanofibrous scaffold can promote neural regeneration, reduce inflammation, and restore spinal cord function in rats. This study provides insights into potential treatment strategies for spinal cord injury using NSCs and bioactive biomaterials.
ACS APPLIED NANO MATERIALS
(2023)
Article
Immunology
Olena Rakhimova, Alexej Schmidt, Marene Landstrom, Anders Johansson, Peyman Kelk, Nelly Romani Vestman
Summary: The response of stem cells from the apical papilla (SCAPs) is compromised when exposed to bacterial stimuli from infected dental root canals in anaerobic conditions, leading to reduced proliferation and production of inflammatory cytokines. Microenvironmental conditions, such as the presence of microorganisms, need to be included in stem cell-mediated endodontic regenerative studies to further enhance the advantages in the field.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Cell Biology
Qianyu Cheng, Chang Liu, Qiuman Chen, Wenping Luo, Tong-Chuan He, Deqin Yang
Summary: Stem cells from the apical papilla (SCAPs) are a promising candidate for tissue regeneration and regenerative endodontic treatment. However, obtaining enough cells from the limited apical papilla tissue and maintaining their primary phenotype is challenging. This study successfully immortalized human SCAPs, allowing for long-term proliferation and retaining mesenchymal and progenitor biomarkers. The immortalized cells showed greater potential for osteogenic differentiation compared to primary cells, making them a potential source for bone tissue engineering.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Green & Sustainable Science & Technology
Pei Feng, Saipu Shen, Yang Shuai, Shuping Peng, Cijun Shuai, Shijie Chen
Summary: In this study, PLLA chains were grafted onto graphene oxide (GO) to improve the interfacial interaction for PLLA/PGA scaffold. The PLLA-grafted GO reduced the interfacial tension and increased the contact surface area between PLLA and PGA, enhancing the interfacial interaction. The PLLA/PGA scaffold containing 1 wt% the PLLA-grafted GO showed significant improvement in tensile and compressive strength, and demonstrated good cytocompatibility for cell adhesion, migration, and proliferation.
SUSTAINABLE MATERIALS AND TECHNOLOGIES
(2023)
Article
Materials Science, Multidisciplinary
Sara Nasser, Mohamad Ibrahim, Yomen Atassi
Summary: Novel wound dressings were prepared using electrospun nanofibrous PLLA mats loaded with aluminum chloride, gentamicin sulphate, and/or lidocaine hydrochloride. The mats showed better hemostatic efficiency and blood absorption capacity compared to gauze bandages, with those loaded with 30%w/w aluminum chloride having the best hemostatic performance. Release kinetics of gentamicin sulphate in PBS solution followed the Korsmeyer-Peppas model.
MATERIALS CHEMISTRY AND PHYSICS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Aline Queiroz, Mariana Taira Wada, Flavia Cristina Perillo Rosin, Cibele Pelissari, Marilia Trierveiler
Summary: This study evaluated the biological properties of human apical papilla cells cultured under different conditions and found that despite reduced properties of cell proliferation and differentiation in serum-free conditions, the cells were still able to maintain their desirable characteristics.
ARCHIVES OF ORAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
P. De Berdt, K. Vanvarenberg, B. Ucakar, C. Bouzin, A. Paquot, V Gratpain, A. Loriot, V Payen, B. Bearzatto, G. G. Muccioli, L. Gatto, A. Diogenes, A. des Rieux
Summary: Traumatic spinal cord injury is a devastating condition that lacks predictable treatments. Stem cell transplantation, particularly from the human dental apical papilla, has shown therapeutic potential in promoting spinal cord repair. This study reveals that the dental papilla reduces inflammation, protects motoneurons, and induces apoptosis of activated macrophages/microglia at the injury site. The therapeutic effects are likely attributed to the secretome of the implanted papilla, which contains immunomodulatory and pro-angiogenic factors produced by stem cells of the apical papilla (SCAP).
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Engineering, Biomedical
Cijun Shuai, Wenjing Yang, Pei Feng, Shuping Peng, Hao Pan
Summary: The addition of PGA into HAP/PLLA scaffold accelerated the degradation process, leading to increased degradation rate and pore formation, which promoted the deposition and growth of bone-like apatite as well as provided a favorable environment for osteoblast adhesion and proliferation. This led to excellent bone defect repair capacity demonstrated by the formation of abundant new bone tissue and blood vessel tissue, ultimately bridging both ends of the defect region after 8 weeks of implantation.
BIOACTIVE MATERIALS
(2021)
Article
Cell & Tissue Engineering
Shengyan Yang, Qing Liu, Shijing Chen, Fuping Zhang, Yaoyin Li, Wenguo Fan, Lijia Mai, Hongwen He, Fang Huang
Summary: This study reveals the impact of apical periodontitis on tooth root development and the underlying mechanism of impaired odontoblastic differentiation of stem cells from apical papilla. The study also develops a novel strategy for dentin regeneration using a nuclear factor I/C-encapsulated extracellular vesicle.
JOURNAL OF TISSUE ENGINEERING
(2022)
Article
Cell Biology
Koyo Takimoto, Matthias Widbiller, Anibal Diogenes
Summary: Research has shown that dental infections can interfere with the differentiation of stem cells, causing different cellular fates and promoting macrophage migration through the upregulation of chemokines. Activation of TLR3 inhibits osteogenic and odontoblastic differentiation, which can be reversed by inhibiting or targeting TLR3 expression.
Article
Biotechnology & Applied Microbiology
Abdullrahman M. Al-Bishari, Bilal A. Al-Shaaobi, Aisha A. Al-Bishari, Mohammed A. Al-Baadani, Liang Yu, Jiating Shen, Lei Cai, Yiding Shen, Zhennan Deng, Peng Gao
Summary: In this study, a multifunctional nanofiber membrane loaded with vitamin D and curcumin was successfully fabricated. It showed potential for enhancing osteoblast proliferation and differentiation, as well as exhibiting superior anti-inflammatory activity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Valeriia Zymovets, Yelyzaveta Razghonova, Olena Rakhimova, Karthik Aripaka, Lokeshwaran Manoharan, Peyman Kelk, Marene Landstrom, Nelly Romani Vestman
Summary: This study investigates the influence of oral bacteria and their supernatants on the cytokine profile and osteogenic potential of stem cells from the apical papilla (SCAP). It is found that oral bacteria can up- and downregulate the immune and inflammatory responses of SCAP and influence its osteogenic potential, which may have implications for the success of regenerative endodontic treatment (RET).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Zuyun Yan, Tianshi Sun, Wei Tan, Zhicheng Wang, Jinpeng Yan, Jinglei Miao, Xin Wu, Pei Feng, Youwen Deng
Summary: In this study, a magnetic magnesium-loaded bone scaffold was prepared by introducing magnetite nanoparticle into the poly(l-lactic acid)/magnesium oxide composite. By enhancing the activity of Mg2+ channel protein (MAGT1) on the surface of bone marrow mesenchymal stem cells (rBMSCs) through the magnetic torque effect under the static magnetic field (SMF), the scaffold promoted the capture of Mg2+ and induced osteogenesis. In vitro and in vivo experiments showed that the magnetic scaffold accelerated the inflow of Mg2+ from the surrounding microenvironment, improving cellular activities, osteogenesis-related gene expression, and mineralization.
