Article
Multidisciplinary Sciences
Erling Hu, Qi Zhang, Sen Shang, Yinan Jiang, Xiaoyun Lu
Summary: The research revealed that 0.1THz radiation can promote cellular endocytosis and transmembrane transport of small molecules, influencing cell membrane permeability. This provides direct evidence for the potential application of THz in regulating cell membrane functions.
Review
Oncology
Celine Prunier, Philippe Chavrier, Mathieu Boissan
Summary: Metastatic progression is regulated by metastasis promoter and suppressor genes. NME1, NME2, and NME4 are reported as metastasis suppressors, and they interact with members of the GTPase dynamin family to control membrane fission and fusion reactions. NME1 and NME2 provide GTP to dynamins at the plasma membrane, driving endocytosis and attenuating signaling, while NME4 interacts with dynamin OPA1 at the mitochondria inner membrane to promote inner membrane fusion and maintain a fused mitochondrial network. These functions of NME family members are closely linked to metastasis and provide insights into the metastasis suppressor role of NME proteins.
CANCER AND METASTASIS REVIEWS
(2023)
Article
Physics, Fluids & Plasmas
Elisa Floris, Andrea Piras, Francesco Saverio Pezzicoli, Marco Zamparo, Luca Dall'Asta, Andrea Gamba
Summary: Molecular sorting is a crucial process in eukaryotic cells that allows for the concentration of specific chemical factors in different cell membrane subregions, giving them distinct chemical identities and functional properties. A recent phenomenological theory proposes that molecular sorting arises from phase separation-driven sorting domains and domain-induced membrane bending, resulting in the formation of lipid vesicles enriched in the sorted molecules. The efficiency of molecular distillation is found to be influenced by the critical size of phase separated domains. Experimental observations show that sorting domains can be classified into unproductive and productive domains, with the latter evolving into vesicles that detach from the membrane system. The study discusses the implications of this classification within the framework of the phenomenological theory and verifies several predictions through numerical simulations. The analysis reveals that sorting is most efficient when the number of sorting domains is close to a minimum, and introduces an operational definition of the critical size of sorting domains to aid in the analysis of experimental data. Comparison with experimental results suggests that the statistical properties of productive and unproductive domains inferred from experimental data align with those predicted by numerical simulations, supporting the hypothesis that molecular sorting is driven by a phase separation process.
Review
Cell Biology
Chayanika Gundu, Vijay Kumar Arruri, Poonam Yadav, Umashanker Navik, Ashutosh Kumar, Veda Sudhir Amalkar, Ajit Vikram, Ravinder Reddy Gaddam
Summary: Endocytosis is a crucial mechanism for cells to perform their functions, and it involves various mechanisms and regulatory proteins. Dynamin, a GTPase, plays a vital role in forming endocytic vesicles and regulating the process. Apart from dynamin, there are other GTPases, membrane proteins, and secondary messengers involved in dynamin-independent endocytosis. The determination of the specific path of endocytosis is complex and challenging to understand.
Article
Biochemistry & Molecular Biology
Lucas Bayones, Maria Jose Guerra-Fernandez, Fernando Hinostroza, Ximena Baez-Matus, Jacqueline Vasquez-Navarrete, Luciana Gallo, Sergio Parra, Agustin D. Martinez, Arlek Gonzalez-Jamett, Fernando D. Marengo, Ana M. Cardenas
Summary: Gain-of-function mutations of dynamin-2 result in centronuclear myopathy by impairing exocytosis, disrupting actin filament formation and impacting the plasmalemma expression of functional proteins in skeletal muscle cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Miki Suemura, Shusaku Shibutani, Hiroyuki Iwata
Summary: Endocytosis plays a crucial role in cancer proliferation and metastasis. Recent studies have shown that endocytosis inhibitors can suppress cancer cell growth and migration. In this study, the endocytosis inhibitor dynasore was found to reduce cell viability and induce apoptosis in multiple cancer cell lines. Dynasore also activated the ATRChk1 DNA damage response and the apoptosis was sensitized by an ATR inhibitor. These findings suggest that endocytosis inhibitors may suppress DNA replication and their anti-cancer effects can be enhanced by DNA damage response inhibitors.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Plant Sciences
Lu Zhang, Yuanming Liu, Qiaohui Wang, Chao Wang, Shikai Lv, Yanzhen Wang, Jianfeng Wang, Yajuan Wang, Jing Yuan, Hong Zhang, Zhensheng Kang, Wanquan Ji
Summary: This study reveals the mechanism of alternative splicing and protein interaction of the wheat R gene TaYRG1, which enhances resistance to Puccinia striiformis f. sp. tritici and triggers cell death. It also identifies the interaction between TaYRG1 and a dynamin-related protein, suggesting a potential role in membrane trafficking and endocytosis pathway.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Medicine, Research & Experimental
Christopher N. Subasic, Neville J. Butcher, Fiona Simpson, Rodney F. Minchin, Lisa M. Kaminskas
Summary: Dynamin II (dynII) plays a significant role in the internalization pathways of endocytic cells, by allowing membrane invaginations to bud off. However, clinically relevant doses of dynII inhibitors can inhibit the uptake of liposomes by endocytic cells in vitro, but are unlikely to significantly affect the pharmacokinetics of long-circulating, co-administered liposomes, as shown by the study that investigated the impact of phenothiazines, PCZ and thioridazine, on in vitro liposome endocytosis and in vivo liposome pharmacokinetics after PCZ infusion in rats.
MOLECULAR PHARMACEUTICS
(2023)
Article
Chemistry, Medicinal
Luke R. Odell, Mark J. Robertson, Kelly A. Young, Andrew B. McGeachie, Annie Quan, Phillip J. Robinson, Adam McCluskey
Summary: The Bis-T series of compounds are potent inhibitors of dynamin GTPase activity. However, their low cell permeability limits their activity. To overcome this, prodrug ester analogues were synthesized. Among them, propionic ester 4 showed the highest efficacy, as it could effectively cross the cell membrane and rapidly hydrolyze into the desired Bis-T-22.
Review
Oncology
Imran Khan, Patricia S. Steeg
Summary: Endocytosis plays a potentially important role in regulating tumor metastasis by influencing cell migration and invasion through various mechanisms. Dysregulation of multiple endocytic proteins in cancer is involved in regulating tumor metastasis. Metastatic-suppressor genes function in part by regulating endocytosis and altering signaling pathways related to metastasis.
BRITISH JOURNAL OF CANCER
(2021)
Article
Plant Sciences
Giulia De Angelis, Camilla Badiali, Laura Chronopoulou, Cleofe Palocci, Gabriella Pasqua
Summary: Most previous studies on endocytosis in plant cells have focused on clathrin-dependent pathways, leaving limited evidence on clathrin-independent pathways. This study investigated the role of dynamin a, a key protein in both types of endocytic processes, in the uptake of PLGA nanoparticles (NPs). The results showed that NPs can enter plant cells and roots through a dynamin-like-independent endocytic pathway.
Article
Cell Biology
Reem Abu Rass, Japhette Esther Kembou-Ringert, Rachel Zamostiano, Avi Eldar, Marcelo Ehrlich, Eran Bacharach
Summary: Tilapia Lake Virus (TiLV) is an emerging virus lethal to tilapia, and its entry into tilapia cells is dependent on cholesterol and the cellular cytoskeleton, but not on the clathrin-coated pits and acidic environment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Nabilah Ibnat, Rowshan Ara Islam, Ezharul Hoque Chowdhury
Summary: Gene therapy using RNA interference for PRKCA oncogene and gene augmentation for PTEN tumor suppressor effectively reduced tumor growth in a mouse model of breast cancer. Combining delivery of PRKCA specific siRNA and PTEN plasmid with CA NPs significantly decreased cellular growth in MCF-7 cell line and had a similar effect in the triple negative MDA-MB-231 breast cancer cell line.
APPLIED SCIENCES-BASEL
(2021)
Article
Multidisciplinary Sciences
Claudia Matthaeus, Kem A. Sochacki, Andrea M. Dickey, Dmytro Puchkov, Volker Haucke, Martin Lehmann, Justin W. Taraska
Summary: This study investigates the key proteins that regulate the curvature of caveolae using correlative super-resolution light and electron microscopy. The authors discover that caveolins, cavins, and EHD2 form a cohesive structural unit, regulated by intermittent associations with Pacsin2 and EHBP1, to control the curvature of caveolae.
NATURE COMMUNICATIONS
(2022)
Article
Neurosciences
Wonchul Shin, Lisi Wei, Gianvito Arpino, Lihao Ge, Xiaoli Guo, Chung Yu Chan, Edaeni Hamid, Oleg Shupliakov, Christopher K. E. Bleck, Ling-Gang Wu
Summary: This study reveals that endocytosis is mainly mediated by calcium-triggered and dynamin-mediated closure of Omega profiles and fusion pores, leading to the formation of Lambda-shaped, Omega-shaped, and O-shaped vesicles, rather than the traditional flat-to-round membrane transformation.
Article
Agriculture, Multidisciplinary
Francesca Truzzi, Giovanni Dinelli, Enzo Spisni, Emanuela Simonetti, Grazia Trebbi, Sara Bosi, Ilaria Marotti
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2020)
Article
Physiology
Marilena Ignesti, Davide Andrenacci, Bettina Fischer, Valeria Cavaliere, Giuseppe Gargiulo
FRONTIERS IN PHYSIOLOGY
(2019)
Article
Agriculture, Multidisciplinary
Enzo Spisni, Maria Chiara Valerii, Luigia De Fazio, Enrica Rotondo, Marcella Di Natale, Elisabetta Giovanardi, Giovanni Posabella, Valeria Bregola, Verena Stenico, Rocco Enrico Sferrazza, Giovanni Dinelli, Sara Bosi
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2020)
Article
Physiology
Elisa Mezzofanti, Marilena Ignesti, Tien Hsu, Giuseppe Gargiulo, Valeria Cavaliere
FRONTIERS IN PHYSIOLOGY
(2019)
Review
Cell Biology
Davide Andrenacci, Valeria Cavaliere, Giovanna Lattanzi
AGEING RESEARCH REVIEWS
(2020)
Article
Cell Biology
Valeria Cavaliere, Giovanna Lattanzi, Davide Andrenacci
Article
Biochemistry & Molecular Biology
Giulia Serafini, Giorgia Giordani, Luca Grillini, Davide Andrenacci, Giuseppe Gargiulo, Valeria Cavaliere
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Cell Biology
Giorgia Giordani, Valeria Cavaliere, Giuseppe Gargiulo, Giovanna Lattanzi, Davide Andrenacci
Summary: The transposon theory of aging suggests that the activation of transposable elements (TEs) in somatic tissues leads to a shortened lifespan. Studies have found that regulation of TEs changes during aging, with a decrease in middle-aged adults and reactivation in older individuals, indicating dynamic regulation of TEs throughout the lifespan.
Article
Biochemistry & Molecular Biology
Francesca Truzzi, Anne Whittaker, Eros D'Amen, Camilla Tibaldi, Antonella Abate, Maria Chiara Valerii, Enzo Spisni, Giovanni Dinelli
Summary: The combination of SPD and EUG can stimulate autophagy, reduce inflammation, and show potential in treating COVID-19 risk groups.
Article
Oncology
Thi-Ngoc Nguyen, Hieu-Huy Nguyen-Tran, Chen-Yun Chen, Tien Hsu
Summary: This study identifies the molecular and cellular mechanisms underlying the interaction between VHL-deficient kidney tubule cells and macrophages in the tumor microenvironment, providing potential targets for the prevention and treatment of ccRCC.
Article
Nutrition & Dietetics
Chiara Ricci, Fernando Rizzello, Maria Chiara Valerii, Enzo Spisni, Paolo Gionchetti, Silvia Turroni, Marco Candela, Federica D'Amico, Renato Spigarelli, Irene Bellocchio, Giovanni Marasco, Giovanni Barbara
Summary: Geraniol, an acyclic monoterpene alcohol, has demonstrated anti-inflammatory and antimicrobial properties and positive effects on gut microbiota in IBS patients. In this study, IBS patients treated with a low-absorbable geraniol food supplement (LAGS) showed a significant reduction in IBS symptoms severity score compared to placebo. Geraniol administration also resulted in changes in gut microbiota composition, with a decrease in Oscillospira and an increase in Faecalibacterium. The findings suggest that LAGS is effective in treating overall IBS symptoms and improving gut microbiota profile, particularly in the IBS mixed subtype.
Article
Biochemistry & Molecular Biology
Barbara Pavan, Anna Bianchi, Giada Botti, Luca Ferraro, Maria Chiara Valerii, Enzo Spisni, Alessandro Dalpiaz
Summary: Eugenol, cinnamaldehyde, and D-limonene, the main components of natural essential oils, have antioxidant and anti-inflammatory properties and can have beneficial effects on the intestine, heart, and nervous system. New analytical procedures were developed to study their pharmacokinetics and ability to penetrate the central nervous system in rats. Eugenol showed the highest permeability in the cerebrospinal fluid (CSF), while cinnamaldehyde could only reach the CSF through intravenous administration and limonene had no ability to penetrate the CSF. Eugenol also increased cell viability and induced dopamine release, suggesting potential beneficial effects against Parkinson's disease after oral administration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Francesca Truzzi, Anne Whittaker, Eros D'Amen, Maria Chiara Valerii, Veronika Abduazizova, Enzo Spisni, Giovanni Dinelli
Summary: Increases in non-communicable and auto-immune diseases have led to research on natural products and the interrelationship between autophagy and inflammation. The study investigates the effects of wheat-germ spermidine and clove eugenol combination supplement on inflammation and autophagy. Results show that the supplement reduces ROS levels and midkine expression, stimulates autophagy, and improves intestinal health.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Tien Hsu, Hieu-Huy Nguyen-Tran, Maria Trojanowska
JOURNAL OF BIOMEDICAL SCIENCE
(2019)