Article
Engineering, Biomedical
Ziying Zhang, Liang Ma, Hui Yuan, Ziman Chen, Yongqin Lv
Summary: A new recognition method for rapid detection of B-type natriuretic peptide (BNP) using molecularly imprinted nanoparticles (nanoMIP) encapsulated with carbon dots is explored. The nanoMIP exhibits high affinity and selectivity toward BNP with a detection limit of 0.208 pg mL(-1). Real serum samples from 160 individuals are analyzed, and the method shows high accuracy and reliability compared to the routine electrochemiluminescence detection method.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Polymer Science
Cesar Caceres, Ewa Moczko, Itsaso Basozabal, Antonio Guerreiro, Sergey Piletsky
Summary: Molecularly imprinted polymer nanoparticles (nanoMIPs) are promising synthetic receptors due to their stability, inexpensive synthesis, and easy implementation. One of the most challenging targets for synthetic receptors are proteins, but an automated solid-phase synthesizer can help optimize the synthesis protocol for nanoMIPs with specificity for proteins. The validation of the protocol includes regenerating a solid-phase, measuring affinity and specificity of nanoparticles, and testing different protocols to improve the yield of high affinity nanoparticles.
Article
Chemistry, Analytical
Dounia Elfadil, Sara Palmieri, Filippo Silveri, Flavio Della Pelle, Manuel Sergi, Michele Del Carlo, Aziz Amine, Dario Compagnone
Summary: This study proposes a fast molecularly imprinted polymer method for the synthesis of maleic hydrazide (MH) and combines it with electrochemical determination for the analysis of MH residues in food. The method exhibits good sensitivity, reusability, and fouling resistance, and can be used for the determination of MH in model solutions and food samples.
MICROCHEMICAL JOURNAL
(2022)
Article
Polymer Science
Stanislav S. Piletsky, Alvaro Garcia Cruz, Elena Piletska, Sergey A. Piletsky, Eric O. Aboagye, Alan C. Spivey
Summary: Current techniques for the solid phase synthesis of molecularly imprinted polymer (MIP) nanoparticles often use amino silanes for template immobilization prior to polymerization. However, a study found that these amino silanes have problematic side reactions that negatively impact the purity and affinity of the polymers. In this case study, iodo silanes were proposed as a superior alternative and were used to successfully synthesize MIPs targeting epitopes of the cancer biomarker EGFR, showing higher purity, specificity, and nanomolar affinity for the target peptide.
Article
Chemistry, Analytical
Elif Esen, Bilgen Osman, M. Nalan Demir
Summary: MLT-imprinted microbeads were prepared for SPE of MLT from edible plants. The MIP showed high adsorption capacity and selectivity for MLT, leading to efficient extraction from various plant samples for HPLC analysis. The SPE method using MLT-PEMATrp microbeads was highly effective and had a low LOD.
MICROCHEMICAL JOURNAL
(2021)
Article
Polymer Science
Sarah H. Megahed, Mohammad Abdel-Halim, Amr Hefnawy, Heba Handoussa, Boris Mizaikoff, Nesrine A. El Gohary
Summary: This study used molecularly imprinted polymers (MIPs) for selective extraction of quinic acid (QA) from coffee bean extract. Computational modeling and equilibrium rebinding studies were performed to optimize the preparation of MIP C, which showed better binding performance than other MIPs tested. The applied molecularly imprinted solid-phase extraction (MISPE) using MIP C was able to selectively extract QA from aqueous coffee extract, with reduced interference from other components.
Article
Materials Science, Biomaterials
Long Jiang, Tao Jing, Xiantao Shen
Summary: Molecularly imprinted wax (MIW) spheres were successfully prepared using octadecanoic acid as the functional monomer and wax as the matrix. The MIW spheres displayed remarkable molecular recognition ability and high selectivity towards the template. The as-synthesized MIW could be dissolved in n-hexane or melted by heating for subsequent fluorescence and mass spectrum analysis without the target elution process.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Review
Polymer Science
Sazlinda Kamaruzaman, Najihah Mohammad Nasir, Siti Munirah Mohd Faudzi, Noorfatimah Yahaya, Nor Suhaila Mohamad Hanapi, Wan Nazihah Wan Ibrahim
Summary: MIPs, synthetic polymers with predetermined selectivity, are ideal for separation processes and have been widely studied in natural product applications.
Review
Polymer Science
Erinn K. Reville, Elizabeth H. Sylvester, Sarah J. Benware, Shreeya S. Negi, Erik B. Berda
Summary: Molecularly imprinted polymers (MIPs) combine the complexity of receptor proteins with the tunability of synthetic research, and have potential in the recognition of target molecules. The aim of MIP research is to achieve similar recognition accuracy as receptor proteins while tailoring functional properties for advanced applications.
Article
Chemistry, Analytical
Abderrahman Lamaoui, Jose Maria Palacios-Santander, Aziz Amine, Laura Cubillana-Aguilera
Summary: A fast and simple strategy based on the microwave technique for the preparation of magnetic molecularly imprinted polymers (MMIPs) is proposed for the selective determination of sulfamethoxazole (SMX). The study involved computational design, synthesis, characterization, and application of MMIPs using different monomers, with experimental results confirming the computational approach. Both MMIPs showed good selectivity and sensitivity for SMX detection, with MMIP-MAA demonstrating higher recovery values in real sample (tap water) analysis compared to MMIP-AM.
Article
Nanoscience & Nanotechnology
Alessandra Maria Bossi, Alessio Bucciarelli, Devid Maniglio
Summary: Nanosized biomimetics prepared by molecular imprinting are showing potential as plastic antibodies in medicine for cell imaging and targeted therapies. Utilizing natural polymer silk fibroin to synthesize biocompatible MIP SF-NPs demonstrates high affinity recognition sites and biocompatibility.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Analytical
Xinyu Wang, Yan Feng, Haiyan Chen, Yan Qi, Jianrong Yang, Shuang Cong, Yongxin She, Xiaolin Cao
Summary: The study prepared dummy template molecularly imprinted polymers using precipitation polymerization method and characterized the obtained polymers. The polymers showed high adsorption capacity, efficiency and specific recognition ability towards N-nitrosamines. A solid phase extraction process based on the polymers coupled with high performance liquid chromatography-tandem mass spectrometry was established and demonstrated good linearity, low limits of detection and quantification, as well as satisfactory recoveries. The method provided a convenient way to enrich and detect N-nitrosamines in complex food matrix.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Yadiris Garcia, Myleidi Vera, Veronica A. Jimenez, Luis F. Barraza, Joao Aguilar, Susana Sanchez, Eduardo D. Pereira
Summary: This study developed a simple and optimized Molecularly Imprinted Nanoparticle-Based Assay (MINA) for the detection of microcystin-leucine-arginine (MC-LR) in water. Molecularly imprinted nanoparticles (MINs) were prepared by solid-phase polymerization and immobilized onto a polystyrene microplate. The MINs showed strong binding affinity for MC-LR and exhibited a limit of detection of 2.49 x 10(-4) nmol L-1.
Article
Chemistry, Multidisciplinary
Hong Chen, Fangsheng Wu, Yibing Xu, Yuan Liu, Lun Song, Xiujuan Chen, Qun He, Wei Liu, Qiaoying Han, Zihua Zhang, Yun Zou, Wenbin Liu
Summary: In this study, molecularly imprinted polymers (MIPs) were successfully synthesized for the extraction and determination of 4-methyldimethcathinone (4-MDMC). The results showed that MIPs exhibited excellent adsorption capacity for the target compound, with imprinting efficiency strongly dependent on temperature and pH, and demonstrated good reusability and regeneration capabilities. This material shows great promise for the rapid extraction and separation of synthetic cathinones dissolved in liquid for criminal sciences applications.
Review
Chemistry, Analytical
Matheus Siqueira Silva, Ana P. M. Tavares, Henrique Dipe de Faria, Maria Goreti Ferreira Sales, Eduardo Costa Figueiredo
Summary: This review discusses the rational design and applications of molecularly imprinted polymers (MIPs) in solid phase extraction of disease biomarkers from biological samples, as well as the remaining challenges and future trends in the application of Molecular Imprinting Technology in sample preparation for clinical diagnosis.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Multidisciplinary
Kapil D. Patel, Hae-Won Kim, Jonathan C. Knowles, Alessandro Poma
ADVANCED FUNCTIONAL MATERIALS
(2020)
Article
Chemistry, Multidisciplinary
Federico Fenaroli, James D. Robertson, Edoardo Scarpa, Virginia M. Gouveia, Claudia Di Guglielmo, Cesare De Pace, Philip M. Elks, Alessandro Poma, Dimitrios Evangelopoulos, Julio Ortiz Canseco, Tomasz K. Prajsnar, Helen M. Marriott, David H. Dockrell, Simon J. Foster, Timothy D. McHugh, Stephen A. Renshaw, Josep Samitier Marti, Giuseppe Battaglia, Loris Rizzello
Article
Biophysics
Arnab Guha, Omar Sheej Ahmad, Antonio Guerreiro, Kal Karim, Niklas Sandstrom, Victor P. Ostanin, Wouter van der Wijngaart, Sergey A. Piletsky, Sourav K. Ghosh
BIOSENSORS & BIOELECTRONICS
(2020)
Article
Multidisciplinary Sciences
Meng Liu, Azzurra Apriceno, Miguel Sipin, Edoardo Scarpa, Laura Rodriguez-Arco, Alessandro Poma, Gabriele Marchello, Giuseppe Battaglia, Stefano Angioletti-Uberti
NATURE COMMUNICATIONS
(2020)
Article
Polymer Science
Cesar Caceres, Ewa Moczko, Itsaso Basozabal, Antonio Guerreiro, Sergey Piletsky
Summary: Molecularly imprinted polymer nanoparticles (nanoMIPs) are promising synthetic receptors due to their stability, inexpensive synthesis, and easy implementation. One of the most challenging targets for synthetic receptors are proteins, but an automated solid-phase synthesizer can help optimize the synthesis protocol for nanoMIPs with specificity for proteins. The validation of the protocol includes regenerating a solid-phase, measuring affinity and specificity of nanoparticles, and testing different protocols to improve the yield of high affinity nanoparticles.
Article
Biophysics
LiangLiang Liu, Fabiana Grillo, Francesco Canfarotta, Michael Whitcombe, Stephen P. Morgan, Sergey Piletsky, Ricardo Correia, ChenYang He, Andrew Norris, Serhiy Korposh
Summary: This study introduces a nanoMIPs-functionalized LPG sensor for highly selective detection of carboxyl-fentanyl in blood, providing a reliable tool for detecting butyrylfentanyl intoxication.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Biochemistry & Molecular Biology
Valeria Mercadante, Edoardo Scarpa, Valeria De Matteis, Loris Rizzello, Alessandro Poma
Summary: Nanotechnology and nanoparticles play a crucial role in modern research, especially in healthcare therapeutic applications. Polymeric nanoparticles show great promise for treating oral diseases, but careful optimization in production and thorough biological considerations and clinical evaluations are necessary for successful clinical application.
Review
Materials Science, Multidisciplinary
Oliver Jamieson, Francesco Mecozzi, Robert D. Crapnell, William Battell, Alexander Hudson, Katarina Novakovic, Ashwin Sachdeva, Francesco Canfarotta, Carmelo Herdes, Craig E. Banks, Helena Snyder, Marloes Peeters
Summary: Antibiotic resistance is a global threat that needs urgent attention. Molecularly imprinted polymers (MIPs) are emerging as a viable alternative to antibiotics due to their low-cost, superior thermal and chemical stability, and simplified manufacturing process. There is a clear need for fast, selective, cost-effective methods to detect antibiotic residues in complex matrices.
PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE
(2021)
Article
Nanoscience & Nanotechnology
Jake McClements, Patrick Marcel Seumo Tchekwagep, Ana Luiza Vilela Strapazon, Francesco Canfarotta, Alan Thomson, Joanna Czulak, Rhiannon E. Johnson, Katarina Novakovic, Patricia Losada-Perez, Azfar Zaman, Ioakim Spyridopoulos, Robert D. Crapnell, Craig E. Banks, Marloes Peeters
Summary: The study introduces a novel functionalized interface utilizing nanoMIPs attached to SPEs for thermal detection of cTnI, enabling early diagnosis of myocardial infarction with potential commercial applications. The developed sensor platform demonstrates high sensitivity, versatility, reproducibility, and cost-effectiveness, highlighting its promising role in sustainable healthcare.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Review
Biochemistry & Molecular Biology
Rui Liu, Alessandro Poma
Summary: Despite significant efforts in the past decades, challenges such as severe side effects and poor bioavailability continue to hinder the clinical translation of drug molecules. As a result, researchers are turning their attention to drug delivery vehicles, with molecularly imprinted polymers (MIPs) showing promise due to their selective recognition, enhanced drug loading, sustained release, and robustness in harsh conditions. This review highlights the design and development of strategies for MIPs used as drug delivery vehicles, focusing on different drug delivery mechanisms, such as rate-programmed, stimuli-responsive, and active targeting, published over the past five years.
Article
Chemistry, Analytical
Alberto Gomez-Caballero, Ainhoa Elejaga-Jimeno, Gontzal Garcia del Cano, Nora Unceta, Antonio Guerreiro, Miquel Saumell-Esnaola, Joan Salles, M. Aranzazu Goicolea, Ramon J. Barrio
Summary: This study describes the production of artificial anti-CB1 antibodies in nanoparticle format using a solid-phase imprinting approach. By selecting a linear C-terminus sequence of the receptor as a template, the artificial antibody showed affinity and selectivity towards the target protein through dot blot and Western blot experiments. The developed molecularly imprinted materials pave the way for novel imprinted nanomaterials for other proteins within the superfamily of G-protein-coupled receptors (GPCR).
Review
Chemistry, Multidisciplinary
Konstantin G. Shevchenko, Irina S. Garkushina, Francesco Canfarotta, Sergey A. Piletsky, Nickolai A. Barlev
Summary: Molecularly imprinted polymers (MIPs) are synthetic polymeric structures that selectively recognize target molecules. They can exceed the affinity and selectivity of natural antibodies and can be fabricated against any target molecule. MIPs can be used for controlled release of therapeutic payloads, making them a potential novel class of therapeutics in medicine.
Review
Polymer Science
Jiahe Han, Alessandro Poma
Summary: Infective endocarditis is a heart disease caused by bacterial infection of heart valves. Antibiotics are the main treatment, but their efficacy is limited by bacterial drug resistance and biofilm formation. To overcome these issues, anti-biofilm treatments that enhance antibiotic action are urgently needed.
Review
Pharmacology & Pharmacy
Xiaohan Ma, Jonathan C. Knowles, Alessandro Poma
Summary: Molecular imprinting technology has made significant advancements in the design and production of MIP nanoparticles (MIP NPs) as antibody substitutes. However, its overall sustainability and biodegradability have been questioned. This review aims to examine the production and purification strategies of MIP NPs from a sustainability and waste management perspective.
Article
Chemistry, Multidisciplinary
Rachel L. Norman, Rajinder Singh, Frederick W. Muskett, Emma L. Parrott, Alessandro Rufini, James Langridge, Franscois Runau, Ashley Dennison, Jacqui A. Shaw, Elena Piletska, Francesco Canfarotta, Leong L. Ng, Sergey Piletsky, Donald J. L. Jones
Summary: This study utilized nano molecularly imprinted polymers (nanoMIPs) to capture the critical oncogene KRAS, allowing for the determination of mutations and discovery of epitopes using LC-MS technology. The approach demonstrated the potential for precise genotyping of cancer cell lines and patients through the use of nanoMIPs, indicating a promising translation from research to clinical utility.