Article
Chemistry, Multidisciplinary
Xiangzhao Ai, Dan Wang, Anna Honko, Yaou Duan, Igor Gavrish, Ronnie H. Fang, Anthony Griffiths, Weiwei Gao, Liangfang Zhang
Summary: Increasing surface heparin density on cellular nanosponges can enhance their inhibition against SARS-CoV-2, providing a facile approach to boost antiviral efficacy by mimicking host cells and utilizing glycan engineering strategies.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Microbiology
Nicolas Malausse, Sylvie van der Werf, Nadia Naffakh, Sandie Munier
Summary: Co-infections between influenza A virus (IAV) and influenza B virus (IBV) were studied by infecting A549 cells with recombinant IAV-GFP and IBV-mCherry. Surprisingly, IBV replication was enhanced when co-infected with IAV, particularly when IAV was added 1 hour before IBV. This enhancing effect was dependent on transcription/replication of the IAV genome.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Hanyuan Zhang, Alanna M. Klose, Benjamin L. Miller
Summary: The study utilized glycan-based receptor analogue microarrays on the AIR platform to achieve label-free, multiplex detection and discrimination of human and avian influenza viruses. The method can be expanded to systematically evaluate influenza virus adaptation in humans, facilitating global influenza surveillance and serving as a new tool enabling quick reactions in the face of a pandemic outbreak for health organizations, governments, research institutes, and laboratories.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Joseph D. Trimarco, Sarah L. Nelson, Ryan R. Chaparian, Alexandra Wells, Nathan B. Murray, Parastoo Azadi, Carolyn B. Coyne, Nicholas S. Heaton
Summary: The study identifies the glycosyltransferase B3GAT1 as a host protein that restricts a broad spectrum of influenza and other respiratory viruses in vitro and in mice when ectopically overexpressed.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Mariana Leguia, Alejandra Garcia-Glaessner, Breno Munoz-Saavedra, Diana Juarez, Patricia Barrera, Carlos Calvo-Mac, Javier Jara, Walter Silva, Karl Ploog, Lady Amaro, Paulo Colchao-Claux, Christine K. Johnson, Marcela M. Uhart, Martha I. Nelson, Jesus Lescano
Summary: Highly pathogenic avian influenza A/H5N1 viruses have invaded the Americas and pose a threat to wildlife, poultry, and humans. The HPAI/H5N1 viruses in Peru have genetic reassortment between Eurasian and American lineages, emphasizing the need for surveillance and control measures.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Corey Momont, Ha V. V. Dang, Fabrizia Zatta, Kevin Hauser, Caihong Wang, Julia di Iulio, Andrea Minola, Nadine Czudnochowski, Anna De Marco, Kaitlin Branch, David Donermeyer, Siddhant Vyas, Alex Chen, Elena Ferri, Barbara Guarino, Abigail E. Powell, Roberto Spreafico, Samantha S. Yim, Dale R. Balce, Istvan Bartha, Marcel Meury, Tristan I. Croll, David M. Belnap, Michael A. Schmid, William Timothy Schaiff, Jessica L. Miller, Elisabetta Cameroni, Amalio Telenti, Herbert W. Virgin, Laura E. Rosen, Lisa A. Purcell, Antonio Lanzavecchia, Gyorgy Snell, Davide Corti, Matteo Samuele Pizzuto
Summary: The study describes a monoclonal antibody, FNI9, targeting neuraminidase, which can effectively inhibit the enzymatic activity of all group 1 and group 2 influenza A viruses, as well as certain influenza B viruses. FNI9 also shows broad neutralizing activity against seasonal influenza viruses and can synergize with antibodies targeting the hemagglutinin stem. The potent prophylactic activity of FNI9 against lethal influenza A and B virus infections in mice supports its development for the prevention of influenza illness.
Article
Immunology
Miao He, Zhihui Ren, Mohsan Ullah Goraya, Yinhong Lin, Jinghan Ye, Rui Li, Jianping Dai
Summary: Apigetrin, an active compound derived from Ixeris sonchifolia Hance, inhibits influenza A virus (IAV) infection by regulating TLR4 and autophagy signaling pathways. It reduces oxidative stress and cytokine storm induced by IAV.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Review
Immunology
Rory D. de Vries, Katja Hoschler, Guus F. Rimmelzwaan
Summary: In this review, the results of an assay to detect influenza virus-specific antibodies that mediate ADCC were summarized. The study found that HA-specific ADCC mediating antibodies can be detected in serum samples from individuals vaccinated or infected with influenza viruses. The presence of cross-reactive antibodies and their immunological functionalities were also investigated. The findings suggest that virus-specific antibodies induced by infection or vaccination have additional immunological functionalities that contribute to protective immunity.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Soheil Rastgou Talemi, Marie Bartenschlager, Bianca Schmid, Alessia Ruggieri, Ralf Bartenschlager, Thomas Hoefer
Summary: This study uncovers the rate-limiting steps in dengue virus replication through single-cell analysis and computational inference, demonstrating the impact of antiviral drugs and vaccines on viral spread.
Article
Cell Biology
Belinda L. Spillings, Christopher J. Day, Albert Garcia-Minambres, Anupriya Aggarwal, Nicholas D. Condon, Thomas Haselhorst, Damian F. J. Purcell, Stuart G. Turville, Jennifer L. Stow, Michael P. Jennings, Johnson Mak
Summary: This study reveals that HIV is captured near the lymphocyte surface in a virion-glycan-dependent manner. Removing certain glycans impairs virus-cell binding and interfering with glycan interactions reduces HIV infectivity. These findings provide new insights into the interaction between HIV and host cells.
Article
Immunology
Sugandha Raj, Jake Astill, Nadiyah Alqazlan, Nitish Boodhoo, Douglas C. Hodgins, Eva Nagy, Samira Mubareka, Khalil Karimi, Shayan Sharif
Summary: This study investigates the transmission of H9N2 avian influenza virus through direct contact between infected and recipient chickens. The results suggest that aerosol route of infection is more effective in establishing infection and leads to higher virus shedding and antibody response compared to direct inoculation.
Article
Immunology
Lan Cao, Ying Lu, Chaojun Xie, Yiyun Chen, Lijun Liang, Tengfei Zhou, Ziyi Zeng, Chen Wen, Biao Di, Baisheng Li, Kuibiao Li, Zhoubin Zhang
Summary: Recent research suggests that influenza C virus (ICV) may have a greater clinical impact than previously believed, but limited knowledge exists compared to influenza A and B viruses due to poor surveillance and inability to propagate. This study reports the first case of ICV infection in mainland China, identified during an influenza A(H3N2) outbreak. Phylogenetic analysis revealed that this ICV was triple reassortant, and serological evidence indicated potential family-clustering infection. Heightened surveillance of ICV prevalence and variation in China is crucial, especially during the COVID-19 pandemic.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Pharmacology & Pharmacy
Yuling Tang, Ruijing Su, Qingyue Gu, Yanxin Hu, Hanchun Yang
Summary: Influenza A virus infection leads to significant morbidity and mortality worldwide, and secondary bacterial infection worsens the severity and fatality of the initial viral infection. The role of mast cells in viral and bacterial co-infection is not well understood.
ANTIVIRAL RESEARCH
(2023)
Article
Virology
Minhui Guan, Alicia K. Olivier, Xiaotong Lu, William Epperson, Xiaojian Zhang, Lei Zhong, Kaitlyn Waters, Nataly Mamaliger, Lei Li, Feng Wen, Yizhi J. Tao, Thomas J. DeLiberto, Xiu-Feng Wan
Summary: In addition to causing outbreaks in domestic poultry, subtype H7 avian influenza A viruses (IAVs) can cause sporadic spillover infections in lower mammals and humans. This study demonstrates that SLe(X) expression varies among wild dabbling ducks. While SLe(X) does facilitate infection of H7 viruses, other types of SA2,3Gal glycan receptors also play a role in shaping the tissue/host tropisms of H7 IAVs in aquatic wild birds.
JOURNAL OF VIROLOGY
(2022)
Article
Immunology
Nguyen Cong Khanh, Ashley L. Fowlkes, Ngu Duy Nghia, Tran Nhu Duong, Ngo Huy Tu, Tran Anh Tu, Jeffrey W. McFarland, Thoa Thi Minh Nguyen, Nga Thu Ha, Philip L. Gould, Pham Ngoc Thanh, Nguyen Thi Huyen Trang, Vien Quang Mai, Phuc Nguyen Thi, Satoko Otsu, Eduardo Azziz-Baumgartner, Dang Duc Anh, A. Danielle Iuliano
Summary: Influenza burden estimates based on hospitalization data in Vietnam show higher rates among children under 5 years old and adults over 65 years old, highlighting the importance of prevention and control measures, such as vaccination, in these high-risk populations.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Chemistry, Medicinal
Sultan N. Baytas, Sony S. Varghese, Weihua Jin, Yanlei Yu, Peng He, Marc Douaisi, Fuming Zhang, Paul Brodfuehrer, Ke Xia, Jonathan S. Dordick, Robert J. Linhardt
Summary: Bovine intestinal heparins differ structurally and in anticoagulant activity from porcine intestinal heparins, but can be enhanced by treatment with 3-O-sulfotransferase. Remodeled bovine intestinal heparins can be used to prepare low molecular weight heparins with similar activity and structure to Enoxaparin. Additionally, heparin-derived Enoxaparin from bovine intestinal sources may have low levels of heparin induced thrombocytopenia.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Review
Pharmacology & Pharmacy
Yejin Kim, Eun Ji Park, Tae Wan Kim, Dong Hee Na
Summary: The review discusses the principles of in vitro release testing methods for biopolymeric particulate systems, including recent research articles and their characteristics such as IVIVC, accelerated release testing methods, and the stability of encapsulated drugs.
Article
Chemistry, Multidisciplinary
Hye Gyeong Yang, Eun-Ju Yang, Eun Ji Park, Young Jin Lee, Maryam Sadat Safavi, Kyung-Sik Song, Dong Hee Na
Summary: This study demonstrates the use of bovine serum albumin nanoparticles (BSA-NPs) to encapsulate beta-carotene (BC) and improve its aqueous solubility. The BSA-NPs exhibited good stability and controlled release, as well as high antioxidant activity. These findings suggest that BSA-NPs could be a promising nanocarrier for the delivery of BC.
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Medicinal
Soo Hyun Kim, Han Ju Yoo, Eun Ji Park, Dong Hee Na
Summary: Nano differential scanning fluorimetry (nanoDSF) is a powerful tool for thermal stability screening and optimal buffer selection in antibody formulations. In this study, the thermal stability of immunoglobulin G (IgG) was investigated in different buffers. The results showed that the sodium acetate buffer at pH 4.6 was suitable for IgG formulation, as it provided the best stability against aggregation. In contrast, the sodium citrate buffer had higher aggregation and viscosity than the sodium acetate buffer at the same pH. Protein-protein interaction studies suggested that the difference in aggregation between acetate and citrate buffers could be explained by attractive intermolecular interactions in citrate buffer and repulsive interactions in acetate buffer.
Article
Chemistry, Multidisciplinary
Dohyun Kim, Daehyun Shin, Jain Koo, Jungseop Kim, Seon Jeong Na, Joon Seok Bang, Dong Hee Na, Jaehwi Lee
Summary: This study investigated the effects of contact time, disk shape, and polymer molecular weight on the mucoadhesive properties of disks made with hydrophilic polymers. The results showed that the mucoadhesive force and work of adhesion values increased with longer contact time, thicker disks, and larger surface areas.
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Adrianne L. L. Gelbach, Fuming Zhang, Seok-Joon Kwon, John T. T. Bates, Andrew P. P. Farmer, Jonathan S. S. Dordick, Chunyu Wang, Robert J. J. Linhardt
Summary: Heparan sulfate (HS) acts as a co-receptor of angiotensin-converting enzyme 2 (ACE2) by interacting with the spike glycoprotein (SGP) of severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), facilitating the entry of the virus into host cells. This study analyzed the interactions between SARS-CoV-2 SGP receptor binding domain (RBD) and heparin using kinetic and structural analysis. The findings suggest that heparin plays a crucial role in the binding of SGP RBDs, with higher affinity observed for the Delta and Omicron variants compared to the wild type. The interactions between SGP RBDs and heparin are pH sensitive, with acidic pH enhancing binding and basic pH reducing binding, particularly for the Omicron variant.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Pharmacology & Pharmacy
Soo Hyun Kim, Han Ju Yoo, Eun Ji Park, Wonhwa Lee, Dong Hee Na
Summary: The study demonstrated that in sodium acetate buffer, the thermal stability and aggregation propensity of IgG increase as buffer concentration decreases. The best stability and lower viscosity were observed in 10 mM sodium acetate buffer.
JOURNAL OF PHARMACEUTICAL INVESTIGATION
(2022)
Article
Chemistry, Multidisciplinary
Hong Taek Kim, Miri Yoo, Eun-Ju Yang, Kyung-Sik Song, Eun Ji Park, Dong Hee Na
Summary: The study aimed to explore the effect of pH on the complexation of quercetin and polyamidoamaine (PAMAM) dendrimers, which possess antioxidant activity in water solutions. Quercetin's therapeutic use has been hindered by its poor water solubility and bioavailability. Complexation of quercetin with generation 4 PAMAM (G4) at different pH values (4-9) revealed pH 6 as the optimal condition in terms of solubility and stability. The water solubility of quercetin in 0.1 M phosphate buffer at pH 6.0 with 1% G4 exceeded 1 mg/ml. Water-soluble G4-quercetin complex (G4-Q) exhibited comparable antioxidant and neuroprotective activities to free quercetin dissolved in methanol. In conclusion, this study highlights the optimized complexation of quercetin with PAMAM dendrimers at pH 6, resulting in a complex with good water solubility, strong antioxidant activity, and neuroprotective activity.
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Jae Woon Son, Jong Mun Son, Ki Ho Hur, Wonhwa Lee, Im-Sook Song, Dong Hee Na
Summary: The purpose of this study was to develop fibrinogen formulation using isothermal chemical denaturation (ICD) technique. 10 formulations of fibrinogen were initially prepared and screened via automated, high-throughput ICD measurements. The optimal formulation of fibrinogen was determined through osmolality measurements and accelerated stability study.
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
(2023)
Review
Chemistry, Medicinal
Jae Cheon Kim, Eun Ji Park, Dong Hee Na
Summary: Recently, two oral-administered peptide pharmaceuticals, semaglutide and octreotide, have been developed and are considered as a breakthrough in peptide and protein drug delivery system development. These two oral peptide products have achieved significant success, but the poor permeability of peptides through the intestinal epithelium remains a major obstacle. Therefore, gastrointestinal permeation enhancers are crucial for the development of oral peptide products.
Review
Pharmacology & Pharmacy
Dohyun Kim, Lan Jin, Eun Ji Park, Dong Hee Na
Summary: Chemical permeation enhancers (CPEs) are commonly used to improve intestinal permeability, but they have unspecified targets and undesired toxicity. In contrast, peptide permeation enhancers (PPEs) modulate specific tight-junction proteins with high efficacy and reversibly increase intestinal permeability, indicating their potential as safe ingredients.
JOURNAL OF PHARMACEUTICAL INVESTIGATION
(2023)
Article
Pharmacology & Pharmacy
Tae Jin Lee, Dohyun Kim, Jae Cheon Kim, Si Won Ro, Dong Hee Na
Summary: The developed dexrabeprazole enteric-coated tablet showed superior stability and similar dissolution as the commercial rabeprazole product with dose-proportional pharmacokinetics.
JOURNAL OF PHARMACEUTICAL INVESTIGATION
(2023)
Article
Biotechnology & Applied Microbiology
Mamta Gupta, Matthew Wong, Kamran Jawed, Kamil Gedeon, Hannah Barrett, Marcelo Bassalo, Clifford Morrison, Danish Eqbal, Syed Shams Yazdani, Ryan T. Gill, Jiaqi Huang, Marc Douaisi, Jonathan Dordick, Georges Belfort, Mattheos A. G. Koffas
Summary: This study successfully produced isobutanol in E. coli by combining in vivo and in vitro metabolic engineering. The in vivo production of alpha-ketoisovalerate (KIV) was achieved through CRISPR-mediated integration and CRISPRi technology. Subsequent in vitro conversion to isobutanol was carried out using engineered enzymes. This two-step approach resulted in high yields and productivities, making it a promising method for producing important metabolites.
METABOLIC ENGINEERING COMMUNICATIONS
(2022)
Article
Pharmacology & Pharmacy
Dohyun Kim, Jain Koo, Eun-Ju Yang, Kyusun Shim, Yee Yee Tin, Ziming Lin, Kyung Taek Oh, Dong Hee Na
Summary: The study aimed to develop a tablet formulation of mesoporous silica particles (MSPs) that can effectively trap and enhance the dissolution of celecoxib. Optimal conditions for drug loading into MSPs were investigated and the crystallinity of the drug was evaluated. The anti-inflammatory activity and dissolution of the MSP tablet were compared with commercial celecoxib. The results showed that the MSP formulation successfully increased drug solubility and exhibited comparable activity to celecoxib.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Biology
Paul S. S. Kwon, Shirley Xu, Hanseul Oh, Seok-Joon Kwon, Andre L. Rodrigues, Maisha Feroz, Keith Fraser, Peng He, Fuming Zhang, Jung Joo Hong, Robert J. J. Linhardt, Jonathan S. S. Dordick
Summary: A polysulfated synthetic drug called suramin binds to the ACE2 receptor and heparan sulfate binding sites on SARS-CoV-2 receptor binding domains (RBDs), with preferential binding for the Omicron RBD and inhibition of infection by the Omicron variant in vitro. These findings suggest that suramin and other polysulfated molecules could be potential therapeutic and prophylactic options against COVID-19.
COMMUNICATIONS BIOLOGY
(2023)