期刊
NATURE METHODS
卷 13, 期 3, 页码 233-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3727
关键词
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资金
- European Union Seventh Framework Programme (FP7-KBBE-7-single-stage) under Promys [613745]
- Novo Nordisk Foundation through the Novo Nordisk Foundation Center for Biosustainability
- Lundbeck Foundation [R77-2010-6772, R140-2013-13496] Funding Source: researchfish
- NNF Center for Biosustainability [Bacterial Cell Factory Optimization, iLoop, Bacterial Synthetic Biology] Funding Source: researchfish
- Novo Nordisk Fonden [NNF13SA0009311, NNF13SA0006019, NNF14OC0011335, NNF10CC1016517] Funding Source: researchfish
We comprehensively assessed the contribution of the Shine-Dalgarno sequence to protein expression and used the data to develop EMOEMOEMOPECEC (Empirical Model and Oligos for Protein Expression Changes; http://emopec.biosustain.dtu.dk). EMOEMOEMOPECEC is a free tool that makes it possible to modulate the expression level of any Escherichia coli gene by changing only a few bases. Measured protein levels for 91% of our designed sequences were within twofold of the desired target level.
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