期刊
NATURE METHODS
卷 13, 期 8, 页码 673-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.3894
关键词
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资金
- NIH [AI085583, GM083898, NS046579, NS081687]
- Human Frontier Science Program
- Canadian Institutes of Health Research [MOP12942, MOP286161]
- McKnight foundation
Although neuronal activity can be modulated using a variety of techniques, there are currently few methods for controlling neuronal connectivity. We introduce a tool (GFE3) that mediates the fast, specific and reversible elimination of inhibitory synaptic inputs onto genetically determined neurons. GFE3 is a fusion between an E3 ligase, which mediates the ubiquitination and rapid degradation of proteins, and a recombinant, antibody-like protein (FingR) that binds to gephyrin. Expression of GFE3 leads to a strong and specific reduction of gephyrin in culture or in vivo and to a substantial decrease in phasic inhibition onto cells that express GFE3. By temporarily expressing GFE3 we showed that inhibitory synapses regrow following ablation. Thus, we have created a simple, reversible method for modulating inhibitory synaptic input onto genetically determined cells.
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