期刊
NATURE METHODS
卷 13, 期 9, 页码 759-762出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3955
关键词
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资金
- Broad Institute
- Burroughs Welcome Fund
- Center for Microbiome Informatics and Therapeutics at MIT
- National Human Genome Research Institute [U54HG003067]
- Center for Environmental Health Sciences at MIT
- Fijian Ministry of Health
We have developed hydrogel-based virtual microfluidics as a simple and robust alternative to complex engineered microfluidic systems for the compartmentalization of nucleic acid amplification reactions. We applied in-gel digital multiple displacement amplification (dMDA) to purified DNA templates, cultured bacterial cells and human microbiome samples in the virtual microfluidics system, and demonstrated whole-genome sequencing of single-cell MDA products with excellent coverage uniformity and markedly reduced chimerism compared with products of liquid MDA reactions.
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