Article
Biochemistry & Molecular Biology
Chia-Ru Chung, Hsin-Yao Wang, Po-Han Chou, Li-Ching Wu, Jang-Jih Lu, Jorng-Tzong Horng, Tzong-Yi Lee
Summary: This study used an ensemble of multiple preprocessing methods to extract critical information from complicated mass spectrometry spectral data for identifying microorganisms and predicting antibiotic resistance. The ensemble method outperformed individual methods, achieving the highest accuracy on independent testing datasets. Important peaks related to antibiotic resistance could be detected, providing valuable information for further investigation of the resistance mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Automation & Control Systems
Alejandro Guerrero-Lopez, Carlos Sevilla-Salcedo, Ana Candela, Marta Hernandez-Garcia, Emilia Cercenado, Pablo M. Olmos, Rafael Canton, Patricia Munoz, Vanessa Gomez-Verdejo, Rosa del Campo, Belen Rodriguez-Sanchez
Summary: A multi-view heterogeneous Bayesian model (KSSHIBA) is proposed for predicting antibiotic resistance using MALDI-TOF MS data and epidemiological differences. This model provides interpretable results and eliminates the need for preprocessing steps by using raw data, reducing the time required for resistance mechanism analysis in microbiological laboratories.
ENGINEERING APPLICATIONS OF ARTIFICIAL INTELLIGENCE
(2023)
Article
Immunology
Achiraya Siriphap, Thawatchai Kitti, Akachai Khuekankaew, Chalermchai Boonlao, Chonthida Thephinlap, Chutamas Thepmalee, Nittiya Suwannasom, Krissana Khoothiam
Summary: This study investigated the prevalence of ESBL-positive E. coli and K. pneumoniae isolates from hospitalized patients in Chiangrai Prachanukroh hospital, Thailand. The study found a high prevalence and emerging antibiotic resistance of ESBL-positive strains, indicating the need for carbapenems and aminoglycosides as the first-line drugs.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Elvira R. Shaidullina, Andrey V. Romanov, Elena Y. Skleenova, Eugene A. Sheck, Marina V. Sukhorukova, Roman S. Kozlov, Mikhail V. Edelstein
Summary: MALDI-TOF mass spectrometry has been widely used for detection of carbapenemases in clinical microbiology. This study developed a sensitive and cost-effective MALDI-TOF MS-based assay for detecting CPE using commercial antibiotic susceptibility-testing disks as a substrate.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Michel Baez, Ivette Espinosa, Alexandra Collaud, Iliana Miranda, Damarys de las Nieves Montano, Angel L. Feria, Rosa Elena Hernandez-Fillor, Dasiel Obregon, Pastor Alfonso, Vincent Perreten
Summary: This study found an increasing number of third-generation cephalosporin-resistant Escherichia coli (3GC-R-Ec) in poultry from seven farms in the Cuban province of Mayabeque. The isolates were found to contain multiple resistance genes, belong to various sequence types and phylogroups, revealing a genetically diverse population of multiresistant ESBL-producing E. coli in Cuban poultry farms.
Article
Agriculture, Dairy & Animal Science
Elena Gonzalez-Fandos, Alba Martinez-Laorden, Ana Abad-Fau, Eloisa Sevilla, Rosa Bolea, Maria Jesus Serrano, Olga Mitjana, Cristina Bonastre, Alicia Laborda, Maria Victoria Falceto, Rafael Pagan
Summary: This study highlights the potential selection of resistant bacteria in pig feces when oxytetracycline or enrofloxacin is used in food-producing animals. It emphasizes the importance of preventing fecal contamination of carcasses during slaughter.
Article
Multidisciplinary Sciences
Chonticha Romyasamit, Phoomjai Sornsenee, Siriphorn Chimplee, Sitanun Yuwalaksanakun, Dechawat Wongprot, Phanvasri Saengsuwan
Summary: The study found ESBL-producing E. coli and K. pneumoniae in raw vegetables, showing high resistance to beta-lactam antibiotics but good sensitivity to beta-lactam/carbapenem antibiotics. The genes carried by these bacteria may be associated with food chains and humans, suggesting that raw vegetables could serve as an important route for the transmission of ESBL-resistant bacteria to humans.
Article
Microbiology
Seda Ozdikmenli Tepeli, Yasemin Numanoglu Cevik, Melike Nur Tosun, Gizem Taylan Yalcin, Burcu Kaya, Dilvin Ipek, Zekiye Bakkaloglu, Husniye Simsek, Nukhet Nilufer Zorba
Summary: This study investigated the carbapenem resistance and biofilm formation properties of Enterobacterales isolates obtained from raw milk. It found that a small percentage of the isolates showed carbapenem resistance and that the majority of the isolates were capable of biofilm formation.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Daria Janiszewska, Malgorzata Szultka-Mlynska, Pawel Pomastowski, Boguslaw Buszewski
Summary: The quick and accurate identification of microorganisms and the study of antibiotic resistance are crucial in various fields. MALDI-TOF MS is a rapidly growing identification method that allows for a broad spectrum of data, providing insights into the characteristics of microorganisms and their resistance mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Yuriko Matsumura, Kazuko Ikegaya
Summary: The worldwide increase in multidrug-resistant microorganisms that produce extended-spectrum beta-lactamases (ESBLs) and carbapenemases is a serious public health concern. Recently, matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as a rapid method for detecting antibiotic-resistant bacteria. This study aimed to establish a method to detect ESBL-producing Escherichia coli using MALDI-TOF MS by monitoring the hydrolyzation of cefotaxime (CTX). The results showed that this method can rapidly and accurately detect ESBL-producing E. coli with high sensitivity.
Article
Infectious Diseases
Anat Shnaiderman-Torban, Shiri Navon-Venezia, Hadar Baron, Wiessam Abu-Ahmad, Haya Arielly, Gal Zizelski Valenci, Israel Nissan, Yossi Paitan, Amir Steinman
Summary: This study investigated the gut colonization of extended spectrum beta-lactamase-producing Enterobacterales (ESBL-PE) in healthy community dogs in Israel. The results indicate that 6.2% of the dogs carried ESBL-PE strains, which possessed diverse resistomes. Coprophagia was identified as a risk factor for ESBL-PE gut colonization.
Article
Infectious Diseases
Antonio Mandujano, Diana Veronica Cortes-Espinosa, Jose Vasquez-Villanueva, Paulina Guel, Gildardo Rivera, Karina Juarez-Rendon, Wendy Lizeth Cruz-Pulido, Guadalupe Aguilera-Arreola, Abraham Guerrero, Virgilio Bocanegra-Garcia, Ana Veronica Martinez-Vazquez
Summary: This study investigated the antimicrobial resistance profile of ESBL-producing E. coli among food-producing animals in Tamaulipas, Mexico. A total of 200 fecal samples were collected and 5.0% of the strains were confirmed as ESBL producers. High percentage of antimicrobial resistance was observed against gentamicin, tetracycline, and streptomycin. The study suggests that food-producing animals may serve as reservoirs and contribute to the spread of ESBL-producing bacteria.
Article
Infectious Diseases
Eva Torres-Sangiao, Brais Lamas Rodriguez, Maria Cea Pajaro, Raquel Carracedo Montero, Noelia Parajo Pazos, Carlos Garcia-Riestra
Summary: Urinary tract infections (UTIs) are common infectious diseases and antibiotic treatment can lead to resistance. This study proposes a method that combines flow cytometry, matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS), and the VITEK 2 system to shorten the diagnosis time of UTIs. The method showed shorter diagnostic time without significant loss of sensitivity.
Article
Chemistry, Analytical
Tianyi Li, Qinghua Zou, Binghua Zhang, Di Xiao
Summary: MALDI-TOF MS technology can accurately identify Escherichia coli and Shigella spp., which is crucial for disease control and prevention. By identifying characteristic peaks of decarboxylation products, we were able to distinguish E. coli from Shigella spp. This technology is advantageous for its high throughput, high quality, and ease of operation, and is of significant value for the diagnosis of E. coli and Shigella-related diseases.
ANALYTICA CHIMICA ACTA
(2023)
Article
Infectious Diseases
Candace Guzman-Cole, Fabian Santiago, Sona Garsevanyan, Suzanne Sindi, Miriam Barlow
Summary: This study revealed that local selective pressures have a greater influence on the population structure of resistance genes in bacterial populations than migration, indicating the potential for antibiotic resistance to be controlled at a regional level.