Expanding the chemical toolbox for the synthesis of large and uniquely modified proteins

Critical Evaluation and Rate Constants of Chemoselective Ligation Reactions for Stoichiometric Conjugations in Water

(2015) Fumito Saito et al.   ACS Chemical Biology

Oxidative Deselenization of Selenocysteine: Applications for Programmed Ligation at Serine

(2015) Lara R. Malins et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Chemical Synthesis of O-Glycosylated Human Interleukin-2 by the Reverse Polarity Protection Strategy

(2015) Yuya Asahina et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Convergent Versus Sequential Protein Synthesis: The Case of Ubiquitinated and Glycosylated H2B

(2015) Mallikanti Seenaiah et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Synthetic Amino Acids for Applications in Peptide Ligation–Desulfurization Chemistry

(2015) Lara R. Malins et al.   AUSTRALIAN JOURNAL OF CHEMISTRY

Acceleration of thiol additive-free native chemical ligation by intramolecular S → S acyl transfer

(2015) J. Schmalisch et al.   CHEMICAL COMMUNICATIONS

Chemical Synthesis of Phosphorylated Ubiquitin and Diubiquitin Exposes Positional Sensitivities of E1-E2 Enzymes and Deubiquitinases

(2015) Somasekhar Bondalapati et al.   CHEMISTRY-A EUROPEAN JOURNAL

Chemical Protein Synthesis Using a Second-Generation N-Acylurea Linker for the Preparation of Peptide-Thioester Precursors

(2015) Juan B. Blanco-Canosa et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Rapid Additive-Free Selenocystine–Selenoester Peptide Ligation

(2015) Nicholas J. Mitchell et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

An oxazetidine amino acid for chemical protein synthesis by rapid, serine-forming ligations

(2015) Ivano Pusterla et al.   Nature Chemistry

Chemical tagging and customizing of cellular chromatin states using ultrafast trans-splicing inteins

(2015) Yael David et al.   Nature Chemistry

Insights into the deselenization of selenocysteine into alanine and serine

(2015) Shahar Dery et al.   Chemical Science

Delivery of mirror image polypeptides into cells

(2015) Amy E. Rabideau et al.   Chemical Science

Peptideo-Aminoanilides as Crypto-Thioesters for Protein Chemical Synthesis

(2014) Jia-Xing Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Traceless Purification and Desulfurization of Tau Protein Ligation Products

(2014) Oliver Reimann et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Traceless Preparation of C-Terminal α-Ketoacids for Chemical Protein Synthesis by α-Ketoacid-Hydroxylamine Ligation: Synthesis of SUMO2/3

(2014) Thomas G. Wucherpfennig et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Rapid Flow-Based Peptide Synthesis

(2014) Mark D. Simon et al.   CHEMBIOCHEM

Solid phase chemical ligation employing a rink amide linker for the synthesis of histone H2B protein

(2014) Muhammad Jbara et al.   CHEMICAL COMMUNICATIONS

Nonenzymatic Polyubiquitination of Expressed Proteins

(2014) Hosahalli P. Hemantha et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Rapid Ligations with Equimolar Reactants in Water with the Potassium Acyltrifluoroborate (KAT) Amide Formation

(2014) Hidetoshi Noda et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Trifluoroethanethiol: An Additive for Efficient One-Pot Peptide Ligation−Desulfurization Chemistry

(2014) Robert E. Thompson et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Expedient Total Synthesis of Small to Medium-Sized Membrane Proteins via Fmoc Chemistry

(2014) Ji-Shen Zheng et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Accelerated chromatin biochemistry using DNA-barcoded nucleosome libraries

(2014) Uyen T T Nguyen et al.   NATURE METHODS

Synthesis and folding of a mirror-image enzyme reveals ambidextrous chaperone activity

(2014) M. T. Weinstock et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Chemical synthesis of the ATAD2 bromodomain

(2014) G. S. Creech et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A novel PEG-based solid support enables the synthesis of >50 amino-acid peptide thioesters and the total synthesis of a functional SUMO-1 peptide conjugate

(2014) Emmanuelle Boll et al.   Chemical Science

Development of New Thioester Equivalents for Protein Chemical Synthesis

(2013) Ji-Shen Zheng et al.   ACCOUNTS OF CHEMICAL RESEARCH

Chemical Synthesis of Biologically Active Monoglycosylated GM2-Activator Protein Analogue UsingN-Sulfanylethylanilide Peptide

(2013) Kohei Sato et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

The Winding Pathway to Erythropoietin Along the Chemistry-Biology Frontier: A Success At Last

(2013) Rebecca M. Wilson et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Convergent Chemical Synthesis of Histone H2B Protein for the Site-Specific Ubiquitination at Lys34

(2013) Peter Siman et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

One-pot total chemical synthesis of human α-synuclein

(2013) Bruno Fauvet et al.   CHEMICAL COMMUNICATIONS

Protein chemical synthesis by serine and threonine ligation

(2013) Y. Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Erythropoietin Derived by Chemical Synthesis

(2013) P. Wang et al.   SCIENCE

Highly efficient solid phase synthesis of large polypeptides by iterative ligations of bis(2-sulfanylethyl)amido (SEA) peptide segments

(2013) Laurent Raibaut et al.   Chemical Science

Highly efficient one-pot ligation and desulfurization

(2013) Tal Moyal et al.   Chemical Science

Systematic Identification of Proteins Binding to Chromatin-Embedded Ubiquitylated H2B Reveals Recruitment of SWI/SNF to Regulate Transcription

(2013) Efrat Shema-Yaacoby et al.   Cell Reports

Chemistry and Biology of the Ubiquitin Signal

(2012) Liat Spasser et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Chemistry as an Expanding Resource in Protein Science: Fully Synthetic and Fully Active Human Parathyroid Hormone-Related Protein (1-141)

(2012) Jianfeng Li et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Reconstitution of the Catalytic Core of F-ATPase (αβ)3γ fromEscherichia coliUsing Chemically Synthesized Subunit γ

(2012) Frank Wintermann et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Chemical Synthesis of an Erythropoietin Glycoform Containing a Complex-type Disialyloligosaccharide

(2012) Masumi Murakami et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

At Last: Erythropoietin as a Single Glycoform

(2012) Ping Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Total Synthesis of Homogeneous Antifreeze Glycopeptides and Glycoproteins

(2012) Brendan L. Wilkinson et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Convergent Chemical Synthesis of Proteins by Ligation of Peptide Hydrazides

(2012) Ge-Min Fang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Towards the Simplification of Protein Synthesis: Iterative Solid-Supported Ligations with Concomitant Purifications

(2012) Vincent Aucagne et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Racemic Protein Crystallography

(2012) Todd O. Yeates et al.   Annual Review of Biophysics

Sequential native peptide ligation strategies for total chemical protein synthesis

(2012) Laurent Raibaut et al.   CHEMICAL SOCIETY REVIEWS

Targeting Deubiquitinases Enabled by Chemical Synthesis of Proteins

(2012) Shimrit Ohayon et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Polymerization Behavior of a Bifunctional Ubiquitin Monomer as a Function of the Nucleophile Site and Folding Conditions

(2012) Tal Moyal et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Chemical Synthesis of Homogeneous Human Glycosyl-interferon-β That Exhibits Potent Antitumor Activity in Vivo

(2012) Izumi Sakamoto et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Ionization state of the catalytic dyad Asp25/25′ in the HIV-1 protease: NMR studies of site-specifically 13C labelled HIV-1 protease prepared by total chemical synthesis

(2012) Vladimir Yu. Torbeev et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Chemical and semisynthesis of posttranslationally modified proteins

(2012) Peter Siman et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Chemical synthesis and X-ray structure of a heterochiral {D-protein antagonist plus vascular endothelial growth factor} protein complex by racemic crystallography

(2012) K. Mandal et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Total Chemical Synthesis of a 304 Amino Acid K48-Linked Tetraubiquitin Protein

(2011) K. S. Ajish Kumar et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Chemical Synthesis of Ubiquitinated Peptides with Varying Lengths and Types of Ubiquitin Chains to Explore the Activity of Deubiquitinases

(2011) Sudhir N. Bavikar et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Total Chemical Synthesis of an Integral Membrane Enzyme: Diacylglycerol Kinase fromEscherichia coli

(2011) Sunanda Lahiri et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Convergent Chemical Synthesis of [Lysine24, 38, 83] Human Erythropoietin

(2011) Suhuai Liu et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Protein Chemical Synthesis by Ligation of Peptide Hydrazides

(2011) Ge-Min Fang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Preformed Selenoesters Enable Rapid Native Chemical Ligation at Intractable Sites

(2011) Thomas Durek et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Reversible Protection Strategy To Improve Fmoc-SPPS of Peptide Thioesters by the N-Acylurea Approach

(2011) Santosh K. Mahto et al.   CHEMBIOCHEM

Chemical Synthesis and Expression of the HIV-1 Rev Protein

(2011) Peter Siman et al.   CHEMBIOCHEM

Native Chemical Ligation Combined with Desulfurization and Deselenization: A General Strategy for Chemical Protein Synthesis

(2011) Philip E. Dawson   ISRAEL JOURNAL OF CHEMISTRY

Rethinking amide bond synthesis

(2011) Vijaya R. Pattabiraman et al.   NATURE

Synthesis of Thiazolidine Thioester Peptides and Acceleration of Native Chemical Ligation

(2011) Julien Dheur et al.   ORGANIC LETTERS

Protein conformational dynamics in the mechanism of HIV-1 protease catalysis

(2011) V. Y. Torbeev et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Traceless Ligation of Cysteine Peptides Using Selective Deselenization

(2010) Norman Metanis et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Towards biomolecular assembly employing extended native chemical ligation in combination with thioester synthesis using an N→S acyl shift

(2010) Tom Ackrill et al.   BIOPOLYMERS

Peptide and protein thioester synthesis via N→S acyl transfer

(2010) Jaskiranjit Kang et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

N-Methylcysteine-mediated total chemical synthesis of ubiquitin thioester

(2010) Lesly A. Erlich et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Bis(2-sulfanylethyl)amino Native Peptide Ligation

(2010) Nathalie Ollivier et al.   ORGANIC LETTERS

PeptidylN,N-Bis(2-mercaptoethyl)-amides as Thioester Precursors for Native Chemical Ligation†

(2010) Wen Hou et al.   ORGANIC LETTERS

Highly Efficient and Chemoselective Peptide Ubiquitylation

(2009) K. S. Ajish Kumar et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

N→SAcyl-Transfer-Mediated Synthesis of Peptide Thioesters Using Anilide Derivatives

(2009) Shugo Tsuda et al.   ORGANIC LETTERS

Chemoselective Ligation and Modification Strategies for Peptides and Proteins

(2008) Christian P. R. Hackenberger et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

An Efficient Fmoc-SPPS Approach for the Generation of Thioester Peptide Precursors for Use in Native Chemical Ligation

(2008) Juan B. Blanco-Canosa et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION