期刊
CHEMICAL INDUSTRY & CHEMICAL ENGINEERING QUARTERLY
卷 21, 期 1, 页码 201-210出版社
ASSOC CHEMICAL ENG
DOI: 10.2298/CICEQ140228031H
关键词
DTT; DCFH-DA; PFN; BPEA-nit; ascorbic acid; oxidative potential
Several cell-free assays are currently used to quantify and detect the reactive oxygen species (ROS). All of them have certain limitations, do not provide direct comparison of results and, to. date, none of these assays have been acknowledged as the most suitable acellular assay and none has yet been adopted for investigation of potential PM toxicity. These assays include DTT, ascorbic acid, DCFH-DA and PFN assays which have been used in measurements of the particles generated from various combustion sources such as diesel engine, wood smoke (or biomass burning) and cigarette smoke, as well as for outdoor measurements. All the probes use different units for expressing redox properties of PM. Also, their reactivity is being triggered by different types of ROS. This limits the direct comparison of the results that are reporting the toxicity of the same aerosol type measured with various probes. This study is evaluating and comparing the various assays in order to develop deeper understanding of their capabilities, selectivity as well as improve understanding of the underlying chemical mechanisms.
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