Article
Biochemistry & Molecular Biology
Anzhela Pavlova, Victoria Yu Savitskaya, Nina G. Dolinnaya, Mayya Monakhova, Anastasia Litvinova, Elena A. Kubareva, Maria Zvereva
Summary: By studying the promoter region of the human TERT oncogene, it was found that G-quadruplexes play a key role in the immortality of cancer cells and that G>A point substitutions can cause destabilization in the G4 structure.
Article
Biochemistry & Molecular Biology
Anzhela V. Pavlova, Nina G. Dolinnaya, Maria I. Zvereva, Elena A. Kubareva, Mayya V. Monakhova
Summary: G-quadruplexes (G4s), the most widely studied alternative DNA structures, play important roles in regulating cellular processes and DNA repair machinery. This study investigated the effect of G4 on the prokaryotic DNA mismatch repair (MMR) pathway from two bacterial sources with different repair mechanisms. The results showed that G4 formation can interfere with DNA cleavage, a basic function of MMR.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jean Baptiste Charbonnier
Summary: An elegant cryo-EM and biophysical study reveals the conformational changes of the E. coli MutS mismatch repair factor. This study provides a nice follow-up to a recent report from the same group, which characterized how MutS scans DNA without initiating repair on correctly base-paired DNA and recruits MutL upon encountering a mismatch.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2022)
Review
Cell Biology
Guy-Franck Richard
Summary: This review discusses the role of mismatch repair in detecting and fixing DNA strand slippage during microsatellite replication, as well as key molecular differences between normal microsatellites and those biased towards expansions. Experimental evidence suggests that the mismatch repair machinery is involved in the process of microsatellite expansions.
Article
Biochemistry & Molecular Biology
Viktoriia Yu. Savitskaya, Vadim V. Strekalovskikh, Viktoriia G. Snyga, Mayya V. Monakhova, Alexander M. Arutyunyan, Nina G. Dolinnaya, Elena A. Kubareva
Summary: The human pathogen Neisseria gonorrhoeae uses homologous recombination for antigenic variation and avoiding immune response. The surface protein pilin (PilE) is regulated by N. gonorrhoeae mismatch repair (MMR) and a G-quadruplex (G4) structure located upstream of the pilE promoter. The study found that ngMutL preferentially binds to pilE G4, which inhibits ngMutL-induced DNA nicking and modulates cleavage positions, suggesting that pilE G4 may regulate the efficiency of pilin antigenic variation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Paulo G. Hofstatter, Daniel J. G. Lahr
Summary: Repairing DNA damage is crucial for cell function, with the mismatch repair system (MMR) playing a key role. The MMR system in eukaryotes is more complex than in bacteria or Archaea, originating from archaea and potentially acquiring other MMR systems from organelles.
JOURNAL OF MOLECULAR EVOLUTION
(2021)
Review
Microbiology
Yue Yuan On, Martin Welch
Summary: The study explores the differences in DNA mismatch repair mechanism between the opportunistic human pathogen Pseudomonas aeruginosa and well-characterized organisms such as Escherichia coli, highlighting the biological implications of these distinctions.
Article
Biochemistry & Molecular Biology
Christopher D. Putnam, Richard D. Kolodner
Summary: MutL family proteins have a conserved structure and mediate DNA mismatch repair. The function of the endonuclease active site and the role of the linker motif in regulating the activity are not well understood. This study proposes that the inhibitory cysteine in the FERC sequence sequesters the active site and suggests a functional interaction between the conserved linker motif and the FERC sequence.
Article
Genetics & Heredity
Viraj Muthye, Cameron D. Mackereth, James B. Stewart, Dennis Lavrov
Summary: Through studying the mt-genomes of octocorals, it was found that they contain the mtMutS gene involved in DNA repair. This gene is unique to the organellar genome of octocorals. The presence of mt-mutS in some octocoral lineages leads to accelerated mt-sequence evolution.
Article
Multidisciplinary Sciences
Ashutosh S. Phadte, Mayuri Bhatia, Hope Ebert, Haaris Abdullah, Essam Abed Elrazaq, Konstantin E. Komolov, Anna Pluciennik
Summary: This study reveals a strand-directed, extrusion-provoked nuclease function of FAN1 that can cleave DNA near triplet repeat extrahelical extrusions and prevent repeat expansion. The antagonistic effects between MMR proteins and FAN1 in disease onset and progression could be explained by their opposing roles in triplet repeat expansion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Mayya Monakhova, Elena A. Kubareva, Kirill K. Kolesnikov, Viktor A. Anashkin, Egor M. Kosaretskiy, Maria Zvereva, Elena A. Romanova, Peter Friedhoff, Tatiana S. Oretskaya, Timofei S. Zatsepin
Summary: Covalent protein capture by reactive DNA derivatives allows for investigation of structural features in DNA-protein recognition. The use of acrylamide-modified DNA duplexes provides excellent selectivity for cysteine trapping, making them valuable tools for studying DNA-protein interactions.
Review
Cell Biology
Gianno Pannafino, Eric Alani
Summary: The MutL family of DNA mismatch repair proteins plays a crucial role in maintaining genomic integrity and in meiotic division, with different MLH subunits exhibiting variations in cellular functions and phenotypes.
Article
Biochemistry & Molecular Biology
Peter J. Chen, Jeffrey A. Hussmann, Jun Yan, Friederike Knipping, Purnima Ravisankar, Pin-Fang Chen, Cidi Chen, James W. Nelson, Gregory A. Newby, Mustafa Sahin, Mark J. Osborn, Jonathan S. Weissman, Britt Adamson, David R. Liu
Summary: The study found that DNA mismatch repair (MMR) hinders prime editing and promotes undesired byproducts, but the efficiency of substitution, small insertion, and small deletion edits can be enhanced by developing PE4 and PE5 prime editing systems. Strategic silent mutations near the intended edit and optimization of prime editor protein also contribute to improved editing outcomes.
Review
Biochemistry & Molecular Biology
Viktoriia Yu Savitskaya, Mayya Monakhova, Iuliia Iakushkina, Irina I. Borovikova, Elena A. Kubareva
Summary: This article discusses the DNA repair systems of Neisseria gonorrhoeae and their importance in pathogenicity, with a focus on the mismatch repair system and the role of MutS and MutL proteins in the regulation of pilin antigenic variation.
BIOCHEMISTRY-MOSCOW
(2022)
Article
Genetics & Heredity
Milagros Ines Ibanez Busseti, Lucia Malvina Margara, Sofia Daiana Castell, Marisa Mariel Fernandez, Emilio Luis Malchiodi, Guillermo Gabriel Montich, Virginia Miguel, Carlos Enrique Argarana, Mariela Roxana Monti
Summary: This study found that MutS interacts with mismatches in DNA, leading to ADP-ATP exchange and conformational changes, initiating mismatch repair. The study also discovered that MutS can associate with primed DNA replication intermediates, and revealed a novel mechanism of MutS-DNA binding.
Article
Multidisciplinary Sciences
Jiaquan Liu, Jeungphill Hanne, Brooke M. Britton, Matthew Shoffner, Aaron E. Albers, Jared Bennett, Rachel Zatezalo, Robyn Barfield, David Rabuka, Jong-Bong Lee, Richard Fishel
SCIENTIFIC REPORTS
(2015)
Article
Multidisciplinary Sciences
Yongmoon Jeon, Daehyung Kim, Juana V. Martin-Lopez, Ryanggeun Lee, Jungsic Oh, Jeungphill Hanne, Richard Fishel, Jong-Bong Lee
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Multidisciplinary Sciences
Kyung Lock Kim, Daehyung Kim, Seongsil Lee, Su-Jeong Kim, Jung Eun Noh, Joung-Hun Kim, Young Chan Chae, Jong-Bong Lee, Sung Ho Ryu
NATURE COMMUNICATIONS
(2016)
Review
Biochemistry & Molecular Biology
Daehyung Kim, Richard Fishel, Jong-Bong Lee
JOURNAL OF MOLECULAR BIOLOGY
(2018)
Article
Chemistry, Multidisciplinary
Won-Ki Cho, Slobodan Jergic, Daehyung Kim, Nicholas E. Dixon, Jong-Bong Lee
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2014)
Article
Genetics & Heredity
Jong-Bong Lee, Won-Ki Cho, Jonghyun Park, Yongmoon Jeon, Daehyung Kim, Seung Hwan Lee, Richard Fishel
Article
Biochemistry & Molecular Biology
Jeungphill Hanne, Brooke M. Britton, Jonghyun Park, Jiaquan Liu, Juana Martin-Lopez, Nathan Jones, Matthew Schoffner, Piotr Klajner, Ralf Bundschuh, Jong-Bong Lee, Richard Fishel
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Biochemical Research Methods
Minhyeok Chang, Mijin Kwon, Sooran Kim, Na-Oh Yunn, Daehyung Kim, Sung Ho Ryu, Jong-Bong Lee
JOURNAL OF BIOMEDICAL OPTICS
(2014)
Article
Biochemistry & Molecular Biology
Won-Ki Cho, Cherlhyun Jeong, Daehyung Kim, Minhyeok Chang, Kyung-Mi Song, Jeungphill Hanne, Changill Ban, Richard Fishel, Jong-Bong Lee
Article
Biochemistry & Molecular Biology
Daehyung Kim, Fahad Rashid, Yeonmo Cho, Manal S. Zaher, Il Hwan Cho, Samir M. Hamdan, Cherlhyun Jeong, Jong-Bong Lee
NUCLEIC ACIDS RESEARCH
(2019)
Article
Multidisciplinary Sciences
Jiaquan Liu, Ryanggeun Lee, Brooke M. Britton, James A. London, Keunsang Yang, Jeungphill Hanne, Jong-Bong Lee, Richard Fishel
NATURE COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Brooke M. Britton, James A. London, Juana Martin-Lopez, Nathan D. Jones, Jiaquan Liu, Jong -Bong Lee, Richard Fishel
Summary: MutS homologs (MSHs) are highly conserved core components of DNA mismatch repair and can form stable sliding clamps on mismatched DNA. By studying the stability, lifetime, and kinetics of MSH sliding clamps in bacteria and humans, a high-throughput Forster resonance energy transfer system has been developed to specifically detect the formation of MSH sliding clamps and can be used for chemical inhibitor screens.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Brooke M. Britton, Remy A. Yovanno, Sara F. Costa, Joshua McCausland, Albert Y. Lau, Jie Xiao, Zach Hensel
Summary: In this study, the structure and dynamics of the E. coli divisome core complex were investigated using various techniques including structure prediction, molecular dynamics simulation, single-molecule imaging, and mutagenesis. The interactions and roles of proteins FtsW, FtsI, FtsQ, FtsL, FtsB, and FtsN in the complex were explored. The findings provide insights into the mechanism of cell wall synthesis activation by FtsN through modulating interactions between FtsI and FtsW.
NATURE COMMUNICATIONS
(2023)