Article
Chemistry, Physical
Sreejith Raveendran, Anindito Sen, Toru Maekawa, D. Sakthi Kumar
Summary: The study explores the deleterious effects of therapeutic gold nanocages on the subcellular organelles of MCF7 cells, demonstrating significant localization of the nanocarriers within specific organelles leading to their destruction within 96 hours.
Article
Biology
Ryuji Yanase, Flavia Moreira-Leite, Edward Rea, Lauren Wilburn, Jovana Sadlova, Barbora Vojtkova, Katerina Pruzinova, Atsushi Taniguchi, Shigenori Nonaka, Petr Volf, Jack D. Sunter
Summary: Attachment to a substrate is a common strategy among eukaryotic parasites for maintaining position in a specific ecological niche. In this study, the researchers used advanced electron microscopy techniques to investigate the adhesion of Leishmania parasites to the stomodeal valve in the sand fly vector. They found distinct structural elements in the attachment plaque and identified five stages of haptomonad-like cell differentiation. The study also demonstrated the importance of calcium for Leishmania adhesion in vitro. These findings provide important insights into the structural and regulatory aspects of Leishmania adhesion and contribute to a better understanding of its life cycle.
Article
Plant Sciences
Jiansheng Guo, Guan Wang, Li Xie, Xinqiu Wang, Lingchong Feng, Wangbiao Guo, Xiaorong Tao, Bruno M. M. Humbel, Zhongkai Zhang, Jian Hong
Summary: This study utilized FIB-SEM technology to investigate the ultrastructural details of tomato spotted wilt virus (TSWV)-infected plant cells for the first time. It revealed several characteristic structures related to viral infection, such as the endoplasmic reticulum membrane network, lipid-like bodies, and connecting structures between cells.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Plant Sciences
Jan Knoblauch, Rainer Waadt, Asaph B. Cousins, Hans-Henning Kunz
Summary: Researchers have established three microscopy protocols to determine the in situ volume of chloroplasts using chlorophyll fluorescence, embedded CFP markers, and calculations from serial block-face scanning electron microscopy. The obtained data were validated by comparing them with mutant lines. The study also found that the size of guard cell plastids is not governed by the same division machinery as mesophyll plastids.
Article
Multidisciplinary Sciences
Jin Tang, Yaodong Wu, Lingyao Kong, Weiwei Wang, Yutao Chen, Yihao Wang, Y. Soh, Yimin Xiong, Mingliang Tian, Haifeng Du
Summary: This study used differential phase contrast scanning transmission electron microscopy to investigate nanoscale magnetic objects in Kagome ferromagnet Fe3Sn2 nanostructures, revealing that these magnetic objects can be attributed to the integral magnetizations of two types of complex 3D magnetic bubbles with depth-modulated spin twisting. While magnetic configurations obtained using high-resolution TEM are generally considered as two-dimensional, the results suggest the importance of the integral magnetizations of underestimated 3D magnetic structures in 2D TEM magnetic characterizations.
NATIONAL SCIENCE REVIEW
(2021)
Article
Multidisciplinary Sciences
Xinye Chen, Kaleigh M. Ryan, Deon Hines, Long Pan, Ke Du, Shiyou Xu
Summary: Visualization of dentin occlusion and quantitative analysis of the depth of occlusion using focused ion beam-scanning electron microscopy tomography provide a novel approach for evaluating oral care products and optimizing dental therapy.
SCIENTIFIC REPORTS
(2023)
Article
Clinical Neurology
Marta Montero-Crespo, Marta Dominguez-Alvaro, Lidia Alonso-Nanclares, Javier DeFelipe, Lidia Blazquez-Llorca
Summary: Alzheimer's disease is a common form of dementia characterized by cognitive impairment, with extracellular protein deposits and abnormal protein accumulation in neurons. Synaptic alterations were found in different stages of the disease, with more severe changes observed in late-stage cases.
Article
Clinical Neurology
Marta Montero-Crespo, Marta Dominguez-Alvaro, Lidia Alonso-Nanclares, Javier DeFelipe, Lidia Blazquez-Llorca
Summary: Alzheimer's disease is a common form of dementia characterized by persistent and progressive impairment of cognitive functions. Early-stage cases show normal synaptic morphology, but late-stage cases experience decreased synaptic density and morphological alterations.
Article
Chemistry, Multidisciplinary
Vinit Sheth, Xuxin Chen, Evan M. Mettenbrink, Wen Yang, Meredith A. Jones, Ons M'Saad, Abigail G. Thomas, Rylee S. Newport, Emmy Francek, Lin Wang, Alex N. Frickenstein, Nathan D. Donahue, Alyssa Holden, Nathan F. Mjema, Dixy E. Green, Paul L. DeAngelis, Joerg Bewersdorf, Stefan Wilhelm
Summary: Super-resolution microscopy was used to visualize the distribution of nanoparticles inside mammalian cells. By exploiting the light scattering properties of nanoparticles, label-free imaging of intracellular nanoparticles was achieved. This technology can be widely used to study the intracellular fate of nanoparticles and inform the development of safer and more effective nanomedicines.
Review
Biochemical Research Methods
Tariq Sami Haddad, Peter Friedl, Navid Farahani, Darren Treanor, Inti Zlobec, Iris Nagtegaal
Summary: The authors discuss three-dimensional imaging pipelines for analyzing archival patient specimens, emphasizing the importance of utilizing these techniques. Advancements in new technologies have improved our ability to visualize tissues with subcellular resolution. Additionally, archival tissues are an overlooked resource that can provide valuable information with the appropriate methods.
Article
Chemistry, Multidisciplinary
Daniele Pirone, Martina Mugnano, Pasquale Memmolo, Francesco Merola, Giuseppe Cesare Lama, Rachele Castaldo, Lisa Miccio, Vittorio Bianco, Simonetta Grilli, Pietro Ferraro
Summary: This study demonstrates a new method for achieving three-dimensional imaging of nanoparticle distribution in flow cytometry, providing new quantitative descriptions for studying NP-cell interactions.
Article
Neurosciences
Yoshio Yamamoto, Kuniaki Sasaki, Misaki Komuro, Takuya Yokoyama, Sayed Sharif Abdali, Nobuaki Nakamuta
Summary: In the rat laryngeal mucosa, laminar nerve endings are distributed and activated by pressure changes in the laryngeal cavity. These nerve endings are suggested to play a role in efferent regulation and have secretory vesicles in the axoplasm. This study analyzed the laminar nerve endings in rats using 3D reconstruction and immunohistochemistry, revealing their interaction with Schwann cells and fibroblasts, as well as their attachment to epithelial cells. It was also found that these nerve endings release glutamate to maintain continuous discharge during the stretching of the laryngeal mucosa.
JOURNAL OF COMPARATIVE NEUROLOGY
(2023)
Article
Dermatology
D. Mirakovski, K. Damevska, V Simeonovski, S. Nikolovska, B. Boev, A. Petrov, T. Sijakova Ivanova, A. Zendelska, M. Hadzi-Nikolova, I Boev, G. Dimov, R. Darlenski, J. Kazandjieva, S. Damevska, M. Situm
Summary: This study investigated the particles adhered to human skin exposed to urban air pollution using SEM-EDX analysis. Various types of particles were found on the skin, including chlorides, organic particles, silicates, carbonates, and metal-rich particles. The results provide new insights into the link between air pollution and skin damage.
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2022)
Article
Spectroscopy
Haiyang Xian, Yiping Yang, Shan Li, Jianxi Zhu, Rui Li, Jieqi Xing, Jiaxin Xi, Hongmei Yang, Xiaoju Lin, Xiao Wu, Hongping He
Summary: Electron tomography (ET) is an effective technique used in structural biology and physical sciences to provide 3D structural information with atomic-scale resolution. This study verifies the applicability of ET in earth and planetary science research and demonstrates its capability to observe the 3D morphology of mineral grains and chemical components distribution in earth and planetary materials.
ATOMIC SPECTROSCOPY
(2022)
Article
Materials Science, Multidisciplinary
Kei-ichiro Uemura, Yoshihiro Miyazono, Tasuku Hiroshige, Keisuke Ohta, Kosuke Ueda, Kiyoaki Nishihara, Makoto Nakiri, Shingo Hirashima, Tsukasa Igawa, Kei-ichiro Nakamura
Summary: This study aimed to clarify the three-dimensional ultrastructure of head-side mice spermatozoa mitochondria. Conventional TEM and FIB/SEM tomography were used to analyze the five mitochondria on the spermatozoa head. The study revealed that the head-most mitochondria have a unique morphology.
MICROSCOPY AND MICROANALYSIS
(2023)