期刊
NANOSCALE
卷 8, 期 33, 页码 15189-15195出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6nr05186c
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- CRUK
- BBSRC [BB/E001777/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [B17092, BB/E001777/1] Funding Source: researchfish
- Cancer Research UK [18604, 11722] Funding Source: researchfish
k-Turns are widespread RNA architectural elements that mediate tertiary interactions. We describe a double-kink-turn motif comprising two inverted k-turns that forms a tight horse-shoe structure that can assemble into a variety of shapes by coaxial association of helical ends. Using X-ray crystallography we show that these assemble with two (dumbell), three (triangle) and four units (square), with or without bound protein, within the crystal lattice. In addition, exchange of a single basepair can almost double the pore radius or shape of a molecular assembly. On the basis of this analysis we synthesized a 114 nt self-complementary RNA containing six k-turns. The crystal structure of this species shows that it forms a quasi-cyclic triangular object. These are randomly disposed about the three-fold axis in the crystal lattice, generating a circular RNA of quasi D-3 symmetry with a shape reminiscent of that of a cyclohexane molecule in its chair conformation. This work demonstrates that the k-turn is a powerful building block in the construction of nano-scale molecular objects, and illustrates why k-turns are widely used in natural RNA molecules to organize long-range architecture and mediate tertiary contacts.
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