Development of a general protoplast-mediated regeneration protocol for Brassica: cabbage and cauliflower as examples

Development of glucoraphanin-rich broccoli (Brassica oleracea var. italica) by CRISPR/Cas9-mediated DNA-free BolMYB28 editing

(2022) Young-Cheon Kim et al.   Plant Biotechnology Reports

Efficient in vitro regeneration of the endangered species Artemisia arborescens L. through direct organogenesis and impact on secondary metabolites production

(2022) Leila Riahi et al.   Horticulture Environment and Biotechnology

Exogenously Applied Polyamines Reduce Reactive Oxygen Species, Enhancing Cell Division and the Shoot Regeneration from Brassica oleracea L. var. capitata Protoplasts

(2021) Agnieszka Kiełkowska et al.   Agronomy-Basel

Efficient Protoplast Regeneration Protocol and CRISPR/Cas9-Mediated Editing of Glucosinolate Transporter (GTR) Genes in Rapeseed (Brassica napus L.)

(2021) Xueyuan Li et al.   Frontiers in Plant Science

Optimization of Protoplast Isolation from Leaf Mesophylls of Chinese Cabbage (Brassica rapa ssp. pekinensis) and Subsequent Transfection with a Binary Vector

(2021) Ganeshan Sivanandhan et al.   Plants-Basel

Establishment of a PEG-mediated protoplast transformation system based on DNA and CRISPR/Cas9 ribonucleoprotein complexes for banana

(2020) Shaoping Wu et al.   BMC PLANT BIOLOGY

Production of nematode free plantlets in Polianthes tuberosa using in vitro culture techniques

(2020) Kanchan B. M. Singh et al.   Horticulture Environment and Biotechnology

Protoplast isolation and culture from Kalanchoë species: optimization of plant growth regulator concentration for efficient callus production

(2019) Jin Cui et al.   PLANT CELL TISSUE AND ORGAN CULTURE

An Efficient and Economical Protocol for Isolating, Purifying and PEG-Mediated Transient Gene Expression of Chinese Kale Hypocotyl Protoplasts

(2019) Sun et al.   Plants-Basel

Application of protoplast technology to CRISPR/Cas9 mutagenesis: from single-cell mutation detection to mutant plant regeneration

(2018) Choun-Sea Lin et al.   PLANT BIOTECHNOLOGY JOURNAL

An efficient mesophyll protoplast isolation, purification and PEG-mediated transient gene expression for subcellular localization in Chinese kale

(2018) Bo Sun et al.   SCIENTIA HORTICULTURAE

A Simple Procedure for Mesophyll Protoplast Culture and Plant Regeneration in Brassica oleracea L. and Brassica napus L.

(2018) N.D. Kaur et al.   Czech Journal of Genetics and Plant Breeding

Peptide Growth Factor Phytosulfokine-α Stimulates Cell Divisions and Enhances Regeneration from B. oleracea var. capitata L. Protoplast Culture

(2018) Agnieszka Kiełkowska et al.   JOURNAL OF PLANT GROWTH REGULATION

Site-directed mutagenesis in Petunia × hybrida protoplast system using direct delivery of purified recombinant Cas9 ribonucleoproteins

(2016) Saminathan Subburaj et al.   PLANT CELL REPORTS

Plant regeneration from protoplasts of Gentiana straminea Maxim

(2016) Guomin Shi et al.   Open Life Sciences

Biotechnological applications in in vitro plant regeneration studies of broccoli (Brassica oleracea L. var. italica), an important vegetable crop

(2015) Pankaj Kumar et al.   BIOTECHNOLOGY LETTERS

Development of a rapid, low-cost protoplast transfection system for switchgrass (Panicum virgatum L.)

(2015) Kellie P. Burris et al.   PLANT CELL REPORTS

High frequency organogenesis in hypocotyl, cotyledon, leaf and petiole explants of broccoli (Brassica oleracea L. var. italica), an important vegetable crop

(2015) Pankaj Kumar et al.   PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS

In vitro regeneration of eight cultivars of Brassica oleracea var. capitata

(2014) Aneta Gerszberg et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

Targeted genome modification of crop plants using a CRISPR-Cas system

(2013) Qiwei Shan et al.   NATURE BIOTECHNOLOGY

Protoplast isolation and plant regeneration of different doubled haploid lines of cauliflower (Brassica oleracea var. botrytis)

(2011) Xiaoguang Sheng et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Regeneration from leaf explants and protoplasts of Brassica oleracea var. botrytis (cauliflower)

(2008) Veera R.N. Chikkala et al.   SCIENTIA HORTICULTURAE