Mammalian Genomic Manipulation with Orthogonal Bxb1 DNA Recombinase Sites for the Functional Characterization of Protein Variants

The structural basis for HIV-1 Vif antagonism of human APOBEC3G

(2023) Yen-Li Li et al.   NATURE

Engineering of NEMO as calcium indicators with large dynamics and high sensitivity

(2023) Jia Li et al.   NATURE METHODS

Expanded ACE2 dependencies of diverse SARS-like coronavirus receptor binding domains

(2022) Sarah M. Roelle et al.   PLOS BIOLOGY

Sensitive genetically encoded sensors for population and subcellular imaging of cAMP in vivo

(2022) Crystian I. Massengill et al.   NATURE METHODS

Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases

(2022) Matthew T. N. Yarnall et al.   NATURE BIOTECHNOLOGY

A Sort-Seq Approach to the Development of Single Fluorescent Protein Biosensors

(2021) John N. Koberstein et al.   ACS Chemical Biology

Streamlined Human Cell-Based Recombinase-Mediated Cassette Exchange Platform Enables Multigene Expression for the Production of Therapeutic Proteins

(2021) Seunghyeon Shin et al.   ACS Synthetic Biology

Mutants of human ACE2 differentially promote SARS-CoV and SARS-CoV-2 spike mediated infection

(2021) Nidhi Shukla et al.   PLoS Pathogens

Extracellular vesicles carry SARS‐CoV‐2 spike protein and serve as decoys for neutralizing antibodies

(2021) Zach Troyer et al.   Journal of Extracellular Vesicles

Apoptolidin family glycomacrolides target leukemia through inhibition of ATP synthase

(2021) Benjamin J. Reisman et al.   Nature Chemical Biology

A highly responsive pyruvate sensor reveals pathway-regulatory role of the mitochondrial pyruvate carrier MPC

(2020) Robinson Arce-Molina et al.   eLife

Comparison of Integrases Identifies Bxb1-GA Mutant as the Most Efficient Site-Specific Integrase System in Mammalian Cells

(2019) Barbara Jusiak et al.   ACS Synthetic Biology

High-performance calcium sensors for imaging activity in neuronal populations and microcompartments

(2019) Hod Dana et al.   NATURE METHODS

An improved platform for functional assessment of large protein libraries in mammalian cells

(2019) Kenneth A Matreyek et al.   NUCLEIC ACIDS RESEARCH

A muscular hypotonia-associated STIM1 mutant at R429 induces abnormalities in intracellular Ca2+ movement and extracellular Ca2+ entry in skeletal muscle

(2019) Jun Hee Choi et al.   Scientific Reports

Multiplex assessment of protein variant abundance by massively parallel sequencing

(2018) Kenneth A. Matreyek et al.   NATURE GENETICS

A multi-landing pad DNA integration platform for mammalian cell engineering

(2018) Leonid Gaidukov et al.   NUCLEIC ACIDS RESEARCH

A platform for functional assessment of large variant libraries in mammalian cells

(2017) Kenneth A. Matreyek et al.   NUCLEIC ACIDS RESEARCH

In Cellulo Mapping of Subcellular Localized Bilirubin

(2016) Jong-Seok Park et al.   ACS Chemical Biology

A method for specifically targeting two independent genomic integration sites for co-expression of genes in CHO cells

(2016) Bahar Baser et al.   METHODS

Crossover-site sequence and DNA torsional stress control strand interchanges by the Bxb1 site-specific serine recombinase

(2016) Ross A. Keenholtz et al.   NUCLEIC ACIDS RESEARCH

GNAS Spectrum of Disorders

(2015) Serap Turan et al.   Current Osteoporosis Reports

STIM1- and Orai1-mediated Ca2+oscillation orchestrates invadopodium formation and melanoma invasion

(2014) Jianwei Sun et al.   JOURNAL OF CELL BIOLOGY

Structural basis for hijacking CBF-β and CUL5 E3 ligase complex by HIV-1 Vif

(2014) Yingying Guo et al.   NATURE

A platform for rapid prototyping of synthetic gene networks in mammalian cells

(2014) Xavier Duportet et al.   NUCLEIC ACIDS RESEARCH

Small molecules that inhibit Vif-induced degradation of APOBEC3G

(2014) Masashi Matsui et al.   Virology Journal

Natural Polymorphisms in Human APOBEC3H and HIV-1 Vif Combine in Primary T Lymphocytes to Affect Viral G-to-A Mutation Levels and Infectivity

(2014) Eric W. Refsland et al.   PLoS Genetics

PEAR: a fast and accurate Illumina Paired-End reAd mergeR

(2013) J. Zhang et al.   BIOINFORMATICS

Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome

(2013) Zhengyao Xu et al.   BMC BIOTECHNOLOGY

Dispersed and Conserved Hydrophobic Residues of HIV-1 Vif Are Essential for CBF  Recruitment and A3G Suppression

(2013) X. Zhou et al.   JOURNAL OF VIROLOGY

DICE, an efficient system for iterative genomic editing in human pluripotent stem cells

(2013) Fangfang Zhu et al.   NUCLEIC ACIDS RESEARCH

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Recombinase-mediated cassette exchange (RMCE) — A rapidly-expanding toolbox for targeted genomic modifications

(2012) Soeren Turan et al.   GENE

Recombinase-Mediated Cassette Exchange (RMCE): Traditional Concepts and Current Challenges

(2011) Soeren Turan et al.   JOURNAL OF MOLECULAR BIOLOGY

The Activity Spectrum of Vif from Multiple HIV-1 Subtypes against APOBEC3G, APOBEC3F, and APOBEC3H

(2011) M. Binka et al.   JOURNAL OF VIROLOGY

Small Molecular Compounds Inhibit HIV-1 Replication through Specifically Stabilizing APOBEC3G

(2010) Shan Cen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Multiplexing RMCE: Versatile Extensions of the Flp-Recombinase-Mediated Cassette-Exchange Technology

(2010) Soeren Turan et al.   JOURNAL OF MOLECULAR BIOLOGY

Identification of 81LGxGxxIxW89 and 171EDRW174 Domains from Human Immunodeficiency Virus Type 1 Vif That Regulate APOBEC3G and APOBEC3F Neutralizing Activity

(2010) Y. Dang et al.   JOURNAL OF VIROLOGY

A Patch of Positively Charged Amino Acids Surrounding the Human Immunodeficiency Virus Type 1 Vif SLVx4Yx9Y Motif Influences Its Interaction with APOBEC3G

(2009) G. Chen et al.   JOURNAL OF VIROLOGY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Antiretroelement Activity of APOBEC3H Was Lost Twice in Recent Human Evolution

(2008) Molly OhAinle et al.   Cell Host & Microbe

Genetically Encoded Calcium Indicators

(2008) Marco Mank et al.   CHEMICAL REVIEWS