期刊
NANO LETTERS
卷 16, 期 4, 页码 2633-2638出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.6b00273
关键词
Super-resolution microscopy; traction force microscopy; mechanobiology; actin cytoskeleton
类别
资金
- Wolfson Imaging Centre
- Medical Research Council [MC_UU_12010, G0902418, MC_UU_12025]
- MRC/BBSRC/EPSRC [MR/K01577X/1]
- Wellcome Trust [104924/14/Z/14]
- Wolfson Foundation
- MRC [G0902418, MR/K01577X/1, MC_UU_12010/9, MC_UU_12010/4] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [1230489] Funding Source: researchfish
- Medical Research Council [MC_UU_12010/9, G0902418, MR/K01577X/1, MC_UU_12010/4] Funding Source: researchfish
Measuring small forces is a major challenge in cell biology. Here we improve the spatial resolution and accuracy of force reconstruction of the well-established technique of traction force microscopy (TFM) using STED microscopy. The increased spatial resolution of STED-TFM (STFM) allows a greater than 5-fold higher sampling of the forces generated by the cell than conventional TFM, accessing the nano instead of the micron scale. This improvement is highlighted by computer simulations and an activating RBL cell model system.
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