期刊
NANO LETTERS
卷 16, 期 3, 页码 1746-1753出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.5b04676
关键词
RNA nanotechnology; RNA nanoparticles; RNA interference; RNA-DNA hybrid reassociation
类别
资金
- Frederick National Laboratory for Cancer Research, National Institutes of Health [HHSN261200800001E]
- UNC Charlotte
- Intramural Research Program of National Institutes of Health, Center for Cancer Research
Current work reports the use of single-stranded RNA toeholds of different lengths to promote the reassociation of various RNA-DNA hybrids, which results in activation of multiple split functionalities inside human cells. The process of reassociation is analyzed and followed with a novel computational multistrand secondary structure prediction algorithm and various experiments. All of our previously designed RNA/DNA nanoparticles employed single stranded DNA toeholds to initiate reassociation. The use of RNA toeholds is advantageous because of the simpler design rules, the shorter toeholds, and the smaller size of the resulting nanoparticles (by up to 120 nucleotides per particle) compared to the same hybrid nanoparticles with single-stranded DNA toeholds. Moreover, the cotranscriptional assemblies result in higher yields for hybrid nanopartides with ssRNA toeholds.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据