期刊
NANO LETTERS
卷 16, 期 1, 页码 624-628出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.5b04354
关键词
DNA strand displacement; G-quadruplex; localized amplification circuits (LAC); DNA origami
类别
资金
- Conseil regional d'Aquitaine
- ANR Vibbnano [ANR-10-NANO-04-03]
- Oligoswitch [ANR-12-IS07-001-01]
- CNRS
- Inserm
DNA circuits tethered to nanoplatforms can perform cascade reactions for signal amplification. One DNA single strand activates a strand-displacement cascade generating numerous outputs, and therefore amplifying the signal. These localized circuits present, however, an important limitation: the spontaneous activation of the cascade reaction. Current methods to stabilize these circuits employ combination of protective DNA strands, which need to be removed to activate the device. This protection-deprotection process generates an important amount of unwanted side reactions. This is indeed an important limitation for the large potential application of these amplification circuits. In the present work, G-quadruplex DNA structures were used to stabilize localized DNA circuits. This new protocol generates nanoplatforms that no longer requires protective-deprotective systems and is therefore completely neutral to the sample. In addition, cations such as Pb2+ or Ca2+ can be also employed to activate the device enlarging the potential applications from biosensors devices to metal detector sensors.
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