Article
Cell Biology
Claire E. Senner, Ziqi Dong, Malwina Prater, Miguel R. Branco, Erica D. Watson
Summary: One-carbon metabolism plays a crucial role in fetal development, but its molecular function is complex and unclear. This study reveals genome-wide epigenetic instability in Mtrr ( gt/gt ) placentas and identifies abnormal DNA methylation and expression of key genes, suggesting a role for histone modifications in epigenetic inheritance.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Oncology
James McDonald, Noor Diab, Elisa Arthofer, Melissa Hadley, Tomas Kanholm, Uzma Rentia, Stephanie Gomez, Angela Yu, Erin E. Grundy, Olivia Cox, Michael J. Topper, Xiaoyun Xing, Pamela L. Strissel, Reiner Strick, Ting Wang, Stephen B. Baylin, Katherine B. Chiappinelli
Summary: The study shows that epigenetic therapies can enhance immune response and recruit immune cells to fight ovarian carcinoma by targeting the epigenome, with a role for p53 in this process. Different types of epigenetic therapy result in upregulation of different repetitive elements, and cell lines with TP53 mutations exhibit weaker response.
Review
Biochemistry & Molecular Biology
Spencer A. Haws, Zoltan Simandi, R. Jordan Barnett, Jennifer E. Phillips-Cremins
Summary: This review discusses the higher-order folding mechanisms of the genome, highlights the links between repetitive sequences and chromatin folding, primarily focusing on mammalian model systems.
Article
Biochemistry & Molecular Biology
Carolyn F. McCabe, Jennifer L. LaBarre, Steven E. Domino, Marjorie C. Treadwell, Ana Baylin, Charles F. Burant, Dana C. Dolinoy, Vasantha Padmanabhan, Jaclyn M. Goodrich
Summary: Maternal prenatal status plays a crucial role in offspring health and disease, and alterations in DNA methylation may be a mechanism through which suboptimal prenatal conditions confer disease risk later in life. This study investigated the association between maternal one-carbon metabolites and DNA methylation patterns in newborns. The findings highlight the importance of maternal diet and cellular methylation potential in shaping offspring health.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Nutrition & Dietetics
Fubin Liu, Huijun Zhou, Yu Peng, Yating Qiao, Peng Wang, Changyu Si, Xixuan Wang, Jianxiao Gong, Kexin Chen, Fangfang Song
Summary: This study conducted a case-control study in China and found a negative association between plasma one-carbon metabolism-related micronutrients and breast cancer risk, mediated by DNA methylation. The selected differential methylation probes were found to mediate the associations between micronutrients and breast cancer risk.
Article
Cardiac & Cardiovascular Systems
Wenwen Li, Li Quan, Kun Peng, Yanru Wang, Xianhua Wang, Quan Chen, Heping Cheng, Qi Ma
Summary: A metabolic switch from fatty acids beta-oxidation (FAO) to glycolysis is a prominent feature of heart failure. Succinate dehydrogenase (SDH) is found to be essential in maintaining the balance of FAO/glycolysis in myocardium. SDH deficiency leads to dilated cardiomyopathy and heart failure, accompanied by a decrease in FAO and an increase in glycolysis. Enforcing FAO through a high-fat diet can reverse these changes and improve heart failure.
BASIC RESEARCH IN CARDIOLOGY
(2023)
Article
Physiology
Soren Impey, Carl Pelz, Lara-Kirstie Riparip, Amanuel Tafessu, Fatema Fareh, Damian G. G. Zuloaga, Tessa Marzulla, Blair Stewart, Susanna Rosi, Mitchell S. S. Turker, Jacob Raber
Summary: The response of the brain to space radiation is a concern for astronauts, and this study found that Si-28 ion irradiation can impair cognitive performance and potentially affect DNA methylation in the hippocampus.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Muath Bishawi, Franklin H. Lee, Dennis M. Abraham, Carolyn Glass, Stephanie J. Blocker, Daniel J. Cox, Zachary D. Brown, Howard A. Rockman, Lan Mao, Tony C. Slaba, Mark W. Dewhirst, George A. Truskey, Dawn E. Bowles
Summary: The complex and inaccessible space radiation environment poses a risk to astronaut cardiovascular health. Exposure to GCR(5-ion) leads to impairment in cardiac function in mice and has implications for health and mortality.
Article
Biochemistry & Molecular Biology
Mengyu Zhang, Weikang Sun, Xiaoxin You, Dongge Xu, Lingling Wang, Jingping Yang, Erguang Li, Susu He
Summary: This study analyzed publicly available datasets and found that EBV-associated gastric cancer (EBVaGC) has a repression of L1 transcription compared with EBV-negative gastric cancer (EBVnGC). Retrotransposition-associated young and full-length L1s (FL-L1s) were found to be the most repressed L1s in EBVaGC. Increased deposition of H3K9me3 on FL-L1s was observed, potentially attributed to increased TASOR expression and the interaction between viral DNA and the TASOR enhancer. This study uncovers a regulation mechanism of L1 expression by chromatin topology remodeling associated with viral-host genome interaction in EBVaGC.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Tong-tong He, Hong-wei Xiao, Maierhaba Wusiman, Dinuerguli Yishake, Ai-ping Fang, Yan Luo, Xiao-zhan Liu, Zhao-yan Liu, Hui-lian Zhu
Summary: The research suggests that a high level of methionine intake may improve survival in patients with newly diagnosed HCC, but no significant association was observed between other micronutrients involved in one-carbon metabolism and HCC survival.
Article
Multidisciplinary Sciences
Sarah Sternbach, Nicole West, Naveen K. Singhal, Robert Clements, Soumitra Basu, Ajai Tripathi, Ranjan Dutta, Ernest J. Freeman, Jennifer McDonough
Summary: The presence of BHMT enzyme in oligodendrocytes was confirmed, and betaine was shown to modulate histone and DNA methyltransferase activity through BHMT in these cells. The availability of methyl donors can impact epigenetic changes and maturation in oligodendrocytes. This study highlights the importance of the BHMT-betaine methylation pathway in regulating epigenetic mechanisms in neurodegenerative diseases.
Article
Plant Sciences
Jaehoon Lee, Seunga Lee, Kyunghyuk Park, Sang-Yoon Shin, Jennifer M. Frost, Ping-Hung Hsieh, Chanseok Shin, Robert L. Fischer, Tzung-Fu Hsieh, Yeonhee Choi
Summary: Through profiling DNA methylation at five stages of Arabidopsis embryogenesis, we found that the gradual increase in mCHH coincides with the expansion of small RNA expression and the spreading of mCHH to nearby sites. We also identified distinct methylation dynamics in different groups of mCHH targets, which are associated with the length, location, and cytosine frequency of transposons.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Suza Mohammad Nur, Mohammed Razeeth Shait Mohammed, Mazin A. Zamzami, Hani Choudhry, Aamir Ahmad, Bushra Ateeq, Irfan A. Rather, Mohammad Imran Khan
Summary: In this study, it was found that tumor cells undergo metabolic reprogramming, including methylation regulation and changes in 1C metabolism, after detachment from the extracellular matrix, promoting cancer cell survival and metastasis.
Article
Environmental Sciences
Yawen Xu, Li Wang, Jun Zhu, Ping Jiang, Zhan Zhang, Lei Li, Qian Wu
Summary: The study found that both Cr (III) and Cr (VI) can interfere with one carbon metabolism, affecting DNA methylation and histone methylation to regulate the expression of neuro-related genes, leading to neurotoxicity.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biochemistry & Molecular Biology
Xingyu Liao, Kang Hu, Adil Salhi, You Zou, Jianxin Wang, Xin Gao
Summary: Repeats are common in the genomes of bacteria, plants and animals, playing crucial roles in evolution, inheritance and genomic stability. Comprehensive identification and classification of repeats can contribute to disease diagnosis, plant improvement and drug development.
NUCLEIC ACIDS RESEARCH
(2022)
Letter
Oncology
Isabelle R. Miousse, Julia Tobacyk, Charles M. Quick, Azemat Jamshidi-Parsian, Charles M. Skinner, Rajshekhar Kore, Stepan B. Melnyk, Kristy R. Kutanzi, Fen Xia, Robert J. Griffin, Igor Koturbash
Article
Nutrition & Dietetics
Katherine F. Wallis, Stepan B. Melnyk, Isabelle R. Miousse
Article
Environmental Sciences
Katherine F. Wallis, Lauren C. Morehead, Jordan T. Bird, Stephanie D. Byrum, Isabelle R. Miousse
Summary: Depletion of methionine and replacement with homocysteine was associated with apoptosis, while depletion of cysteine was associated with ferroptosis. Both methionine and cysteine depletion led to activation of the NRF2 antioxidant pathway, but only cysteine depletion resulted in increased lipid peroxidation. Glutamine depletion showed comparable gene expression patterns and a decrease in glutathione levels, ultimately leading to apoptosis. In this experiment, a strong ATF4-driven ferroptotic gene signature was insufficient to induce ferroptosis without a significant decrease in glutathione levels.
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
(2021)
Article
Biochemistry & Molecular Biology
Regina L. Binz, Ratan Sadhukhan, Isabelle R. Miousse, Sarita Garg, Igor Koturbash, Daohong Zhou, Martin Hauer-Jensen, Rupak Pathak
Summary: Studies have shown that methionine deficiency inhibits tumor growth, but it can also impact healthy immune cells and increase chromosomal aberrations. In mouse experiments, maintaining a methionine-deficient diet led to more chromosomal aberrations in bone marrow cells. Caution is needed when considering implementing a methionine-deficient diet alongside conventional cancer therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Se-Ran Jun, Marjan Boerma, Zulema Udaondo, Sasha Richardson, Karla D. Thrall, Isabelle R. Miousse, John Seng, Rupak Pathak, Martin Hauer-Jensen
Summary: The study found different discovery times in metabolomics pattern after radiation-induced GI injury between male and female adult Rhesus monkeys, leading to restricted detailed analyses only in males. Radiation resulted in increased fatty acid oxidation and circulating levels of corticosteroids, as well as changes in amino acid metabolism, with the most significant changes observed at days 9 and 10 post-irradiation.
Article
Biochemistry & Molecular Biology
Sarita Garg, Tarun K. Garg, Stephen Y. Wise, Oluseyi O. Fatanmi, Isabelle R. Miousse, Alena V. Savenka, Alexei G. Basnakian, Vijay K. Singh, Martin Hauer-Jensen
Summary: This study evaluated the effects of the vitamin E analog gamma-tocotrienol (GT3) on GI recovery in nonhuman primates (NHPs) after total-body irradiation. The results showed that GT3 promoted the survival and proliferation of intestinal stem cells, reduced cell apoptosis, and had a radioprotective function. Therefore, GT3 has the potential to be used as a prophylactic medical countermeasure for radiation-induced GI injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nutrition & Dietetics
Martin J. J. Ronis, Horacio Gomez-Acevedo, Kartik Shankar, Leah Hennings, Neha Sharma, Michael L. Blackburn, Isabelle Miousse, Harry Dawson, Celine Chen, Kelly E. Mercer, Thomas M. Badger
Summary: This study found that soy infant formula does not have estrogenic effects or significantly alter male reproductive development in newborn male piglets, compared to sow milk and milk formula.
Article
Multidisciplinary Sciences
Veronica Miro Pina, Julio Nava-Trejo, Andras Tobias, Etienne Nzabarushimana, Adrian Gonzalez-Casanova, Ines Gonzalez-Casanova
Summary: Preventive and modeling approaches for COVID-19 pandemic often neglect the heterogeneity in population contact structure and individual connectivity, which significantly affect the effectiveness of interventions.
Article
Gastroenterology & Hepatology
Etienne Nzabarushimana, Haixu Tang
Summary: CDI is a GI infection that can be reversed through modulation of the gut microbiota. This study evaluated the diagnostic capabilities of the fecal microbiome on CDI, showing that the species/function composition of the gut microbiome has a robust diagnostic prediction of the disease. The impact of antibiotic therapy on CDI prediction was also assessed, with positive outcomes observed following successful FMT.
Article
Biochemistry & Molecular Biology
Sarita Garg, Tarun K. Garg, Isabelle R. Miousse, Stephen Y. Wise, Oluseyi O. Fatanmi, Alena Savenka, Alexei G. Basnakian, Vijay K. Singh, Martin Hauer-Jensen
Summary: Exposure to high doses of radiation can cause gastrointestinal injury, and there are currently no effective therapies available for mitigating this damage. Gamma-tocotrienol (GT3) is being investigated as a potential radioprotector, but its effectiveness in accelerating gastrointestinal recovery has not been well-studied. This study examined the effects of GT3 in nonhuman primates exposed to partial-body irradiation and found that it had some protective effects in reducing intestinal injury and promoting cell proliferation, although its impact on citrulline levels was minimal.
Article
Biochemistry & Molecular Biology
Tarun K. Garg, Sarita Garg, Isabelle R. Miousse, Stephen Y. Wise, Alana D. Carpenter, Oluseyi O. Fatanmi, Frits van Rhee, Vijay K. Singh, Martin Hauer-Jensen
Summary: Radiation exposure causes acute damage to hematopoietic and immune cells. A study on nonhuman primates has shown that GT3 can accelerate hematopoietic recovery and aid in the recovery of circulating neutrophils and platelets, suggesting its potential as a medical countermeasure for radiation-induced injury to the hematopoietic system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nutrition & Dietetics
Kirsten Clement, Mitchell R. R. McGill, Isabelle R. R. Miousse, Sean G. G. Young, Stepan Melnyk, Igor Koturbash
Summary: This study investigated the effects of MSM on DNA methylation in human HepaRG cells. The results showed that MSM can serve as a donor for methyl groups and induce significant changes in DNA, without affecting global DNA methylation levels or redistribution of DNA methylation patterns.
JOURNAL OF DIETARY SUPPLEMENTS
(2023)
Article
Oncology
Lauren C. Morehead, Sarita Garg, Katherine F. Wallis, Camila C. Simoes, Eric R. Siegel, Alan J. Tackett, Isabelle R. Miousse
Summary: The study found that restricting methionine in the diet increased the expression of MHC-I and PD-L1 in colorectal cancer cells, as well as the expression of STING and interferon. This led to a better response to immune checkpoint inhibitors in a mouse model of colorectal cancer.
Meeting Abstract
Oncology
Lauren C. Morehead, Alan J. Tackett, Isabelle R. Miousse
Meeting Abstract
Environmental Sciences
I. R. Miousse
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
(2020)
Article
Biotechnology & Applied Microbiology
C. F. M. Menck, R. S. Galhardo, A. Quinet
Summary: Studies have shown that xeroderma pigmentosum variant (XP-V) patients have mutations in the POLH gene, resulting in a high frequency of skin tumors. However, it is paradoxical that the translesion synthesis DNA polymerase eta (Pol η) in these patients can actually suppress mutations, and the mechanism behind this is still unclear. Recent evidence suggests that cyclobutane pyrimidine dimers (CPDs) play an instructional role for Pol η, enabling accurate replication of these lesions, and the mutagenic effects induced by UV radiation are caused by the deamination of C-containing CPDs. This process leads to C>T transitions, which are the most common mutations in skin cancers. The delayed replication in XP-V cells amplifies the deamination of C in CPDs and increases the burden of C>T mutations through the activity of backup TLS polymerases.
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
(2024)