Article
Agriculture, Dairy & Animal Science
Wenjun Yan, Rongbin Qiu, Fuyan Wang, Xue Fu, Hao Li, Pengfei Cui, Yaru Zhai, Chun Li, Lan Zhang, Kui Gu, Lei Zuo, Changwei Lei, Hongning Wang, Xin Yang
Summary: Avian infectious bronchitis (IB), caused by avian infectious bronchitis virus (IBV), is a highly contagious disease that poses a serious threat to the poultry industry worldwide. A novel recombinant virus, CK/CH/SCMY/160315, derived from different live attenuated vaccine strains, has led to outbreaks in vaccinated chicken populations in China with various symptoms such as listlessness and sneezing. Pathogenicity analysis confirmed the high infectivity and harmfulness of this new strain.
Article
Veterinary Sciences
Chenyan Wang, Bo Hou
Summary: Despite vaccine use, novel strains and variants of infectious bronchitis virus (IBV) have continuously emerged, causing economic losses to the poultry industry worldwide. This study characterized the IBV isolate CK/CH/GX/202109 from yellow broilers in Guangxi, China. Recombination and 21 mutations were found in the isolate's 1ab gene. Pathological assessment showed high mortality and multiple organ tropisms in infected chickens, emphasizing the need for continued surveillance to control this infection.
VETERINARY RESEARCH
(2023)
Article
Veterinary Sciences
Qi Wu, Zhixian Lin, Jinsen Wu, Kun Qian, Hongxia Shao, Jianqiang Ye, Aijian Qin
Summary: The study developed a peptide enzyme-linked immunosorbent assay (pELISA) as a possible alternative to the neutralization test for evaluating the immune response to IBV vaccine. The pELISA could indirectly evaluate neutralizing antibody titers against different types of IBV in all tested sera, with a coefficient of 0.83 for neutralizing antibody titers. The pELISA has the potential to detect antibodies against different types of IBV in all tested sera and surveillance the infection of IBV.
BMC VETERINARY RESEARCH
(2021)
Article
Veterinary Sciences
Hyun-Jin Kim, Hyuk-Chae Lee, Andrew Y. Y. Cho, Yun-Jeong Choi, Heesu Lee, Dong-Hun Lee, Chang-Seon Song
Summary: This study isolated two novel recombinant infectious bronchitis viruses (IBVs) from chicken farms in Korea. Comparative sequence analysis revealed that these viruses were produced by genetic reassortment between different genotypes of IBV. These findings suggest that the antigenic profiles of IBVs can be altered through natural recombination, highlighting the importance of enhanced surveillance and research for effective countermeasures.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Immunology
Huiling Gong, Ruiqi Ni, Rongbin Qiu, Fuyan Wang, Wenjun Yan, Kailu Wang, Hao Li, Xue Fu, Liang Chen, Changwei Lei, Hong-ning Wang, Xin Yang
Summary: A recombinant strain of avian infectious bronchitis virus (IBV), CK/CH/MY/2020, was isolated from southwest China. This strain belongs to the GI-28 genotype and is a recombination product of three live attenuated vaccine strains (H120, 4/91, and LDT3-A). Pathogenicity analysis showed that CK/CH/MY/2020 is more virulent than the attenuated vaccine strains.
MICROBIAL PATHOGENESIS
(2022)
Article
Virology
Sarah Keep, Phoebe Stevenson-Leggett, Giulia Dowgier, Katalin Foldes, Isobel Webb, Albert Fones, Kieran Littolff, Holly Everest, Paul Britton, Erica Bickerton
Summary: Infectious bronchitis virus is a significant concern for the global poultry industry. Live-attenuated vaccines are generated by serial passage of a virulent isolate in embryonated eggs until attenuation is achieved. However, the exact mechanisms of attenuation are unknown and there is a risk of reversion to virulence.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
So-Youn Youn, Ji-Youn Lee, You-Chan Bae, Yong-Kuk Kwon, Hye-Ryoung Kim
Summary: In this study, genetic and pathogenic characterization of recombinant IBVs isolated from chickens in South Korea between 2003 and 2019 was undertaken. Recombinant IBVs may be more likely to cause trachea infections than the reference strain GI-19, while showing lower pathogenicity in the kidney. The pathogenicity of the recombinant IBVs also varied according to the genotype of the major parent strain.
Article
Biochemistry & Molecular Biology
Kui Gu, Zengxu Song, Peng Ma, Ziwei Liao, Ming Yang, Changyu Zhou, Chao Li, Yu Zhao, Hao Li, Xin Yang, Changwei Lei, Hongning Wang
Summary: A rapid, simple, sensitive, and specific competitive ELISA (cELISA) was developed in this study for the detection of Avian coronavirus-infectious bronchitis virus (AvCoV-IBV) antibodies. The developed cELISA showed high consistency with commercial ELISA, fast operation, and high sensitivity, making it potential for disease monitoring and control.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Martijn A. Langereis, Irina C. Albulescu, Judith Stammen-Vogelzangs, Morindy Lambregts, Ken Stachura, Suzan Miller, Angela M. Bosco-Lauth, Airn E. Hartwig, Stephanie M. Porter, Michelle Allen, Mark Mogler, Frank J. M. van Kuppeveld, Berend-Jan Bosch, Paul Vermeij, Ad de Groof, Richard A. Bowen, Randy Davis, Zach Xu, Ian Tarpey
Summary: A vaccine based on Venezuelan equine encephalitis virus was developed to induce neutralizing antibody responses in guinea pigs and cats, preventing the spread of SARS-CoV-2 among felines. Vaccinated cats did not transmit the virus to other cats, indicating that the vaccine effectively prevented infection and transmission.
Article
Microbiology
Yi Jiang, Mei Xue, Mengjun Tang, Di Zhang, Yan Yu, Sheng Zhou
Summary: The research focused on the evolution of infectious bronchitis virus (IBV) which has restricted cell and tissue tropism. After serial passaging, a Vero cell-adapted strain named HV80 was obtained, which showed extended cell tropism. Whole genome sequencing revealed 19 amino acid point mutations in the viral genome, including 9 mutations in the S gene's second furin cleavage site, which may be associated with the extended tropism of HV80.
VETERINARY MICROBIOLOGY
(2023)
Article
Virology
Mengjiao Huang, Yuan Liu, Chuangchao Zou, Yazhuo Tan, Zhenling Han, Chunyi Xue, Yongchang Cao
Summary: This study identified a novel recombinant strain of infectious bronchitis virus (IBV) in China, named GD17/04, which showed a unique combination of genetic sequences from different IBV lineages. This new strain, GD17/04, caused high morbidity and mortality rates in chickens, and its S1 gene composition provides a basis for further research on the interaction mechanism between IBV and avian hosts.
Article
Agriculture, Dairy & Animal Science
Wenjun Yan, Qingcheng Yang, Siyu Huang, Song Liu, Kailu Wang, Yizhi Tang, Cangwei Lei, Hongning Wang, Xin Yang
Summary: In this study, an IBV strain called AH-2020 was isolated from chickens vaccinated with H120 and 4/91 in Anhui, China. Genetic analysis showed that AH-2020 shares low similarities with the vaccine strains H120, LDT3-A, and 4/91. The study also revealed that AH-2020 infection caused clinical symptoms in chickens and damaged various tissues.
Article
Agriculture, Dairy & Animal Science
Mengjiao Huang, Xuewei Zheng, Yunjing Zhang, Ruohan Wang, Xiaona Wei
Summary: This study investigated the impact of virulent or avirulent IBV infection on the avian host by analyzing the protein expression in chicken kidneys. The results showed distinct expression profiles between different IBV strains, with the IFN-I signaling pathway emerging as a critical response to IBV infection.
Article
Immunology
Yu Zhang, Yuan Yuan, Li-Hua Zhang, Dan Zhu, Lu Wang, Lan-Ping Wei, Wen-Sheng Fan, Chang-Run Zhao, Yan-Jing Su, Jian-Qi Liao, Lu Yong, Tian-Chao Wei, Ping Wei, Mei-Lan Mo
Summary: This study evaluated virus-like particles (VLPs) carrying S, M, and E proteins from a dominant IBV strain in China. The results demonstrated that SME-VLPs induced strong humoral, cellular, and mucosal immune responses, offering effective protection against IBV.
Article
Microbiology
Mengjiao Huang, Yuan Liu, Yongbo Xia, Jingjing Wang, Xuewei Zheng, Yongchang Cao
Summary: This study reveals that infectious bronchitis virus (IBV) suppresses the innate immune response in avian hosts by interfering with the type I interferon signaling pathway through its nucleocapsid (N) protein.
VETERINARY MICROBIOLOGY
(2023)