期刊
VETERINARNI MEDICINA
卷 68, 期 9, 页码 359-367出版社
CZECH ACADEMY AGRICULTURAL SCIENCES
DOI: 10.17221/42/2023-VETMED
关键词
dairy sheep; mastitis; PCR; Streptococcus strains
This study investigated the species, antimicrobial resistance, and virulence genes of Streptococcus in sheep milk with subclinical mastitis. The results showed that S. uberis was the most common identified species, with the highest resistance to cloxacillin and the highest sensitivity to florfenicol. Different combinations of virulence genes were detected in the isolates.
Streptococcus (S.) species are important pathogens that cause mastitis in sheep. The study aimed to examine Streptococcus species in sheep milk with subclinical mastitis, assessing their prevalence, antimicrobial resistance, and virulence genes. A total of 200 milk samples were collected from sheep farms in tzmir's five districts. Out of 32 (28.6%) Streptococcus isolates identified by phenotypic methods, 25 were genotypically identified as S. uberis, 5 as S. agalactiae, and 2 as S. dysgalactiae. Disk diffusion was used to determine the antimicrobial resistance of the isolates. PCR was employed to identify antimicrobial resistance and virulence genes in the isolates. The highest resistance was found for cloxacillin (100%), and the highest sensitivity was found for florfenicol (84%). The most common resistance gene combination was tetM+tetS (3/32) for S. uberis in 9.4%. A total of five virulence genes were detected. GapC+sua (56.2%) constituted the most common gene pattern. The highest virulence gene gapC was detected in 78.1% (25/32) of the isolates. The cylE gene was not detected (0%) in the isolates. Streptococcus species may play a role in mastitis in sheep, emphasising the need for meticulous hygienic milking practices.
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