Article
Plant Sciences
Keith E. Duncan, Kirk J. Czymmek, Ni Jiang, August C. Thies, Christopher N. Topp
Summary: This study presents technical advances in using lab-based X-ray microscopy for high-resolution 3D imaging of plant samples at multiple scales. Serial imaging and improved sample preparation methods allow for the generation of sub-micron 3D volumes co-registered with lower magnification scans, providing explicit contextual reference. This method bridges the imaging gap between light and electron microscopy and can be applied to various economically and scientifically important plant systems.
Article
Biochemical Research Methods
Lidan Fridman, Dvir Yelin
Summary: By using spectrally encoded flow cytometry (SEFC), we can image the deformations of red blood cells in human capillaries, reflecting their true physiological conditions at these important locations.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Chemistry, Analytical
Mario Sanhueza, Rosario del P. Castillo, M. F. Melendrez, Carlos von Plessing, Joanna Tereszczuk, German Osorio, Carlos Pena-Farfal, Marcos Fernandez, Jose Y. Neira
Summary: A novel hyperspectral imaging technique combining confocal laser scanning microscopy and multivariate analysis is proposed for direct identification and quantification of active pharmaceutical ingredients in synthetic tablets. The method is non-destructive, rapid, and highly sensitive, with successful results achieved through multivariate curve resolution-alternating least squares and partial least squares regression models for analysis of pharmaceutical mixtures. This study evaluates the performance of CLSM in quantifying APIs and demonstrates the potential application of hyperspectral imaging for qualitative and quantitative analysis of solid samples using chemometrics.
MICROCHEMICAL JOURNAL
(2021)
Article
Optics
Yongjin Sung, Weizhong Wang
Summary: We present the implementation of hyperspectral confocal microscopy in the short-wave infrared (SWIR) range using a wavelength-scanning laser combined with laser scanning confocal microscopy. The SWIR confocal microscopy enables high-resolution inspection of an IC chip, and the combination of hyperspectral imaging and chemometric analysis allows for identification of functional circuit block groups in the acquired image. The developed instrument has the potential for inline inspection and non-invasive failure analysis of IC chips.
Article
Veterinary Sciences
Ana Luisa Montoya-Navarrete, Alma Lilian Guerrero-Barrera, Teodulo Quezada-Tristan, Arturo G. Valdivia-Flores, Maria J. Cano-Rabano
Summary: Evaluation of red blood cell morphology through microscopy and scanning electron microscopy is important for predicting, evaluating, and monitoring organ functioning. This study assessed the normal red blood cell morphology in different age groups of dogs and investigated the effects of age, sex, and body size on erythrocyte morphometry. The findings provide valuable information for future research and disease diagnosis that require evaluation of red blood cell morphology.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemical Research Methods
Shicheng Hao, Ying Xiong, Sisi Guo, Jing Gao, Xiaotong Chen, Ruoyu Zhang, Lihui Liu, Jianfeng Wang
Summary: This study reports the development and grading performance validation of a low-cost algorithms-based hyperspectral imaging (aHSI) system for radiodermatitis assessment. The system utilizes Monte Carlo simulations to derive hyper-spectra and physiological parameters of the skin, and achieves high classification accuracy using a one-dimensional convolutional neural network (CNN).
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Food Science & Technology
Liliana Perez-Lavalle, Antonio Valero, Manuel Cejudo-Gomez, Elena Carrasco
Summary: Fresh strawberries are popular and consumed frequently, but outbreaks caused by food-borne pathogens have been associated with their consumption. Salmonella enterica subsp. enterica serovar Thompson can form biofilms on the surface of strawberries during storage, which allows the bacteria to survive and spread. This is crucial for food safety.
Article
Optics
Jakob G. P. Lingg, Thomas S. Bischof, Bernardo A. Arus, Emily D. Cosco, Ellen M. Sletten, Christopher J. Rowlands, Oliver T. Bruns, Andriy Chmyrov
Summary: The development of fluorophores with photoemission beyond 1000 nm provides the opportunity to develop novel fluorescence microscopes sensitive to those wavelengths. Imaging at wavelengths beyond the visible spectrum enables imaging depths of hundreds of microns in intact tissue, making this attractive for volumetric imaging applications.
LASER & PHOTONICS REVIEWS
(2023)
Article
Anatomy & Morphology
Heitor Hussni Casarin, Vicente Silva Mattos, Jarbas Caiado de Castro Neto, Michelle Alexandra Chinelatti
Summary: The study evaluated the chemical and morphological changes of femtosecond laser-irradiated enamel using Raman spectroscopy, CLSM, and SEM. Results showed a significant decrease in carbonate content and carbonate/phosphate ratio in enamel after laser irradiation, without altering its structure and morphology.
MICROSCOPY RESEARCH AND TECHNIQUE
(2021)
Article
Engineering, Mechanical
M. Gaier, H-T Lin, Z. N. Farhat, K. P. Plucknett
Summary: This study demonstrates the feasibility of using a heat treatable binder phase for TiC-based cermets, showing a significant increase in hardness and scratch resistance with heat treatment. The research provides insights into the effects of heat treatments on stainless steels in metal ceramic composites.
Article
Biology
George Vaisey, Priyam Banerjee, Alison J. North, Christoph A. Haselwandter, Roderick MacKinnon
Summary: Piezo1 is a stretch-activated Ca2+ channel in red blood cells that regulates cellular volume. Using super-resolution and electron microscopy, we investigated the organization of Piezo1 in red blood cells. We found that Piezo1 exhibits a non-uniform distribution on the cell surface, with a bias towards the biconcave "dimple". The diffusion trajectories of Piezo1 molecules also indicate a preference for the dimple, which can be explained by curvature coupling between the Piezo dome and the cell membrane. Furthermore, Piezo1 does not form clusters with itself or colocalize with other cellular components. These findings suggest that Piezo1 acts as a force-through-membrane sensor for curvature and lateral tension in red blood cells.
Article
Chemistry, Multidisciplinary
Kevin M. Roccapriore, Qiang Zou, Lizhi Zhang, Rui Xue, Jiaqiang Yan, Maxim Ziatdinov, Mingming Fu, David G. Mandrus, Mina Yoon, Bobby G. Sumpter, Zheng Gai, Sergei Kalinin
Summary: The study uses a combination of machine learning and first-principles calculations to reveal the electronic structure changes in adatom arrays on the Co3Sn2S2 cleaved surface. By classifying the atoms based on their structure, it further reveals the unexpected inhomogeneity of electronic structures among atoms in similar configurations, suggesting the presence of multiple types of atoms on the surface.
Article
Biophysics
Xiaocui Zhao, Renkang Zhu, Max Anikovskiy, Qingxi Wu, Zhifeng Ding
Summary: This study utilized scanning electrochemical microscopy (SECM) to quantitatively determine the extracellular H2O2 released from single COS-7 cells with high spatial resolution. The SECM mode allowed for efficient recording of probe approach curves (PACs) and visualization of cell topography simultaneously. The H2O2 concentration at the membrane surface of intact COS-7 cells was determined to be 0.020 mM using experimental PACs and a simulated PAC with a known H2O2 release value. Additionally, confocal microscopy was used to demonstrate the intracellular H2O2 profile, showing that H2O2 generation is centered at the endoplasmic reticula.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Microbiology
Sabina Matveev, Victoria Reingold, Eden Yossef, Noa Levy, Chandrasekhar Kottakota, Guy Mechrez, Alex Protasov, Eduard Belausov, Nitsan Birnbaum, Michael Davidovitz, Dana Ment
Summary: Direct contact between EPF conidia and the host is crucial for infection, and EPF can infect cryptic insects through direct treatment and transmission from infested surfaces. The study investigated the transmission mechanism of conidia from treated surfaces to host eggs and larvae in the case of the red palm weevil (RPW). Results showed that conidial powder treatment significantly reduced hatching rate and larval survival, while conidial suspension treatment had a lower impact on hatching rate. Females transferred conidia to laying holes in both treatments, resulting in reduced egg hatching and larval mortality. Dry conidia showed stronger effects, possibly due to better adhesion to the female weevil. This dissemination mechanism can be explored as a prevention strategy in date plantations in future studies.
Review
Chemistry, Multidisciplinary
Colin J. R. Sheppard
Summary: Various optical methods of super-resolution microscopy, including confocal microscopy, structured illumination, nonlinear microscopy, and image scanning microscopy, are reviewed for their ability to observe delicate biological samples without strong invasive interaction. The discussion also covers the concepts of resolution and the basic principles of superresolution.
APPLIED SCIENCES-BASEL
(2021)