Article
Biotechnology & Applied Microbiology
Pedro Silva Couto, Dale J. J. Stibbs, Marco C. C. Rotondi, Yasuhiro Takeuchi, Qasim A. A. Rafiq
Summary: This study demonstrates a scalable manufacturing process using a microcarrier-bioreactor system for the expansion of gene-modified human mesenchymal stromal cells (hMSCs). The transduced and expanded cells met the criteria established by the International Society for Cell and Gene Therapy (ISCT). This work provides a foundation for the scalable manufacturing of gene-modified hMSCs.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Natalie Vallant, Bynvant Sandhu, Karim Hamaoui, Maria Prendecki, Charles Pusey, Vassilios Papalois
Summary: This study compared the paracrine effects of MSC supernatants from different age and genotype sources on macrophages, revealing distinct patterns of inflammatory marker expression. These findings are crucial for preclinical and clinical studies utilizing MSCs for inflammation and allo-response mitigation in transplant patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Chuqiang Huang, Qiancheng Zhou, Dingshuo Duan, Luo Yu, Wei Zhang, Zhouzhou Wang, Jin Liu, Bowen Peng, Pengfei An, Jing Zhang, Liping Li, Jiaguo Yu, Ying Yu
Summary: This study successfully prepared the Fe-NiSOH catalyst with rapid self-reconstruction properties via a simple oxidation strategy, which can be used for efficient and stable water/seawater oxidation reactions and demonstrated good stability at commercially required current densities.
ENERGY & ENVIRONMENTAL SCIENCE
(2022)
Article
Cell Biology
Noreen Mumtaz, Amel Dudakovic, Asha Nair, Marijke Koedam, Johannes P. T. M. van Leeuwen, Marion P. G. Koopmans, Barry Rockx, Andre J. J. van Wijnen, Bram C. J. van der Eerden
Summary: Arboviruses like Zika virus affect bone development by targeting osteoblasts and altering bone remodeling. In this study, the researchers investigated the effects of Zika virus infection on mesenchymal stromal cells (MSCs) at different stages of osteogenesis. They found that late-stage infected MSCs showed elevated osteogenic markers, calcium content, and altered gene expression compared to early-stage infected MSCs.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Cell & Tissue Engineering
Yujun Sun, Chunyu Xue, Haoyu Wu, Changchuan Li, Shixun Li, Jiankai Luo, Taihe Liu, Yue Ding
Summary: Articular cartilage injury is a common problem in different conditions, but current treatments are not able to fully regenerate damaged cartilage. Mesenchymal stromal cells (MSCs) have potential for cartilage regeneration, but they face challenges such as hypertrophic differentiation and reduced chondrogenic ability in an inflammatory environment. Genetic engineering strategies have been proposed to enhance stem cell-based therapy, and this review summarizes recent research on this topic. The efficacy and safety of different genetic modification strategies, including viral and nonviral delivery transduction, are discussed, as well as the challenges and prospects for clinical translation of genetically modified MSCs.
STEM CELLS AND DEVELOPMENT
(2023)
Article
Chemistry, Physical
Da Xu, Chun Sun, Jiachen Han, Hu Zhang, Jiaqi Tao, Tong Wei, Chao Fan, Zihui Zhang, Wengang Bi
Summary: Compared to 3D perovskites, single-layer 2D perovskites exhibit high stability, high exciton binding energy, and structural flexibility. However, their emission peaks are limited to the blue-green region due to the strong quantum confined effect. This study demonstrates a method to achieve stable red-emitting perovskites through doping ions and surface passivation, providing new synthetic strategies for high-performance perovskite/lanthanide composites.
JOURNAL OF ALLOYS AND COMPOUNDS
(2022)
Article
Chemistry, Multidisciplinary
Chen Lu, Eunkyung Cho, Zhiyuan Cui, Yuhang Gao, Wenjuan Cao, Jean-Luc Bredas, Veaceslav Coropceanu, Feng Li
Summary: In contrast to closed-shell luminescent molecules, organic luminescent radicals have both spin doublet electronic states, resulting in spin-allowed radiative transitions. The efficiency and stability of donor-acceptor (D-A center dot) type luminescent radicals, which have a donor group covalently attached to an electron-withdrawing radical core, remain challenging to define. Experimental and theoretical results suggest that the luminescence efficiency and stability of the radicals are determined by the degree of conjugation and the number of imine nitrogen atoms in the substituents, with higher conjugation and lower number of imine nitrogen atoms leading to higher efficiency and stability.
ADVANCED MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Myeon-Sik Yang, Min-Jung Park, Junhyeong Lee, Byungkwan Oh, Kyung Won Kang, Yeonhwa Kim, Sang-Myeong Lee, Je-Oh Lim, Tae-Yang Jung, Jong-Hwan Park, Seok-Chan Park, Yun-Sook Lim, Soon B. Hwang, Kwang-Soo Lyoo, Dong-il Kim, Bumseok Kim
Summary: In this study, the researchers demonstrated that intratracheal administration of an appropriate amount of AAV2/8 can effectively target lung tissue. The AAV-mediated gene delivery induced the expression of the desired protein in lung parenchymal cells, and this technique was used to develop a SARS-CoV-2 susceptible mouse model. The study also showed the involvement of type I interferon signaling in the protection against SARS-CoV-2.
Article
Energy & Fuels
Yanqi Ren, Bowen Li, Ying Zhu, Zhihao Zhang, Qi Luo, Hongxin Weng, Xin Tan, Peng Xiang, Xinyu Tan
Summary: Perovskite solar cells have become the standard representative of the third generation of photovoltaic power generation due to their low energy consumption and low cost. This study proposes the use of potassium pentaminodifluorobenzoate (KAFA) to improve the performance of perovskite solar cells by enhancing light transmittance and passivating interface defects. The KAFA-treated device achieves a champion power conversion efficiency of 22.46% and shows excellent stability after continuous illumination for 120 hours.
Article
Virology
Lei He, Jiaying Zhong, Guichang Li, Zhengfang Lin, Peijing Zhao, Chuhua Yang, Hairong Wang, Yuhao Zhang, Xiaoyun Yang, Zhongfang Wang
Summary: This study established an efficient and stable system using Newcastle disease virus (NDV) as a vector to generate vaccines against SARS-CoV-2 in animals. The results showed that intramuscular immunization with rNDV-S induced the highest level of binding and neutralizing antibodies, as well as a strong S-specific T-cell response in mice. Intranasal immunization with rNDV-S1 mainly elicited a robust T-cell response. Overall, the NDV-vectored vaccine candidates were able to induce profound humoral and cellular immunity.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Chemistry, Physical
Jihai Duan, Xianxin Fang, Chaojie Li, Jianing Qu, Lexin Guo, Yuanqiang Zou, Mengqi Xiang, Weiwen Wang
Summary: Photocatalytic microreactors have advantages such as large specific surface area, rapid mass transfer, continuous flow operation capability, and low safety hazards, which promote their application in water purification. A monolithic microreactor has been proposed for depositing Ag/AgCl photocatalysts on the surface of the polydopamine-modified melamine sponge backbone. Experimental results showed that the monolithic microreactor can completely degrade methylene blue under visible light irradiation within 10 minutes and degrade 250 L m-2 of methylene blue per hour. The monolithic microreactor also demonstrated long-term reusability through a simple in situ chemical recovery method.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Cell & Tissue Engineering
Yukiya Takayama, Kosuke Kusamori, Yuri Katsurada, Shu Obana, Shoko Itakura, Makiya Nishikawa
Summary: The researchers successfully suppressed the lung entrapment of intravenously administered MSCs and improved their homing to the injured liver by modifying the surface of MSCs with polyethylene glycol (PEG). This modification method has potential clinical applications.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Nanoscience & Nanotechnology
Liying Zhang, Tianle Guo, Boyuan Liu, Du Du, Shendong Xu, Haiying Zheng, Liangzheng Zhu, Xu Pan, Guozhen Liu
Summary: This study presents a solution to the unsatisfactory crystallization of CsPbI3 perovskite films, which is a challenge in the development of high-quality perovskite films. The use of pyrrolidine hydroiodide (PI) with hydrogen bonding improves the crystallization conditions and prompts the transition to CsPbI3 perovskite phase. Devices based on PI-CsPbI3 perovskite films demonstrate high efficiency and stability, providing inspiration for the convenient fabrication of high-quality perovskite films in industry.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Nanoscience & Nanotechnology
Renjie Chen, Biyu Long, Song Wang, Yuning Liu, Jueyao Bai, Sumei Huang, Huili Li, Xiaohong Chen
Summary: Surface modification engineering plays a crucial role in enhancing the crystallinity and passivating the interface and grain boundary of perovskite solar cells (PSCs), resulting in an improvement in power conversion efficiency (PCE) and stability. The introduction of n-type semiconductor bathocuproine (BCP) at the perovskite/transport layer interfaces showed significant enhancements in PCEs due to improved crystallinity and superior interface contact properties. Dual-sided BCP modification at both interfaces further improved crystallinity and contact properties, leading to higher PCEs and enhanced stability of the PSCs.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Engineering, Environmental
Mukaila A. Ibrahim, Tawfik A. Saleh
Summary: The newly designed AC-D/PVP composite material shows potential value in efficiently inhibiting shale hydration in drilling operations, maintaining stability at high temperatures, and effectively inhibiting shale swelling.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Letter
Hematology
Alice Pievani, Samantha Donsante, Chiara Tomasoni, Alessandro Corsi, Francesco Dazzi, Andrea Biondi, Mara Riminucci, Marta Serafini
Editorial Material
Surgery
Alberto Sanchez-Fueyo, Francesco Dazzi
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Review
Virology
Dirk Lindemann, Sylvia Huetter, Guochao Wei, Martin Loechelt
Summary: Foamy viruses (FVs) within the family of Retroviridae exhibit unique features in their molecular biology, such as differences in Gag, Env, and Pol proteins compared to other retroviruses, leading to their classification into a separate subfamily. The specialized Pol encapsidation strategy and unique interactions between Gag, Pol, and Env proteins complicate FV assembly and release mechanisms. Understanding these differences may provide insights into virus envelopment evolution and characteristics within the Retrovirales.
Article
Virology
Zeli Zhang, Mario Perkovic, Qinyong Gu, Kannan Balakrishnan, Anucha Sangwiman, Dieter Haeussinger, Dirk Lindemann, Carsten Muenk
Summary: This study demonstrated that A3B strongly inhibits SIVmac and HIV-2 infectivity, which is counteracted by their Vif proteins. Additionally, the accessory protein Bet of PFV also significantly antagonizes the anti-SIVmac activity of A3B, but unlike Vif, it does not induce the degradation of A3B.
Review
Cell Biology
Luise Mueller, Antje Tunger, Manja Wobus, Malte von Bonin, Russell Towers, Martin Bornhaeuser, Francesco Dazzi, Rebekka Wehner, Marc Schmitz
Summary: Mesenchymal stromal cells (MSCs) have the ability to regulate and inhibit the function of immune cells, playing a crucial role in the pathogenesis of inflammatory disorders and showing promise in the treatment of conditions such as acute graft-versus-host disease, graft rejection, and autoimmune diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Charlotte Steenblock, Stefanie Richter, Ilona Berger, Marko Barovic, Janine Schmid, Undine Schubert, Natalia Jarzebska, Anne von Maessenhausen, Andreas Linkermann, Annette Schuermann, Jessica Pablik, Thomas Dienemann, Katja Evert, Roman N. Rodionov, Natalia Y. Semenova, Vsevolod A. Zinserling, Raul R. Gainetdinov, Gustavo Baretton, Dirk Lindemann, Michele Solimena, Barbara Ludwig, Stefan R. Bornstein
Summary: This study comprehensively analyzed pancreatic tissues from COVID-19 patients and found detectable SARS-CoV-2 viral infiltration in beta-cells, potentially contributing to metabolic dysregulation. Even in the absence of overt new-onset diabetes, viral infection of pancreatic beta-cells may lead to varying degrees of metabolic abnormalities.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Vipul Sheth, Victoria Potter, Hugues de Lavallade, Shreyans Gandhi, Austin Kulasekararaj, Pramila Krishnamurthy, Varun Mehra, Francesco Dazzi, Ghulam Mufti, Antonio Pagliuca, Donal Mclornan, Kavita Raj
Summary: The study found that pre-emptive DLI is an effective strategy in lowering the risk of relapse in patients with hematological malignancies. Patients who received DLI treatment had significantly improved overall survival, while those who did not receive treatment had poor outcomes.
BLOOD CANCER JOURNAL
(2021)
Article
Immunology
Johannes Schetelig, Falk Heidenreich, Henning Baldauf, Sarah Trost, Bose Falk, Christian Hossbach, Ruben Real, Axel Roers, Dirk Lindemann, Alexander Dalpke, Martin Kolditz, Katja de With, Martin Bornhaeuser, Ezio E. Bonifacio, Elke Ruecker-Braun, Vinzenz Lange, Jan Markert, Ralf Barth, Jan A. Hofmann, Juergen Sauter, Stefanie N. Bernas, Alexander H. Schmidt
Summary: HLA genotype does not play a major role in determining the severity of COVID-19, as indicated by the findings of this study. The spike glycoprotein alone may allow for robust T-cell responses regardless of the HLA genotype.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Marco Romano, Raul Elgueta, Daniel McCluskey, Ana Maria Ortega-Prieto, Emilie Stolarczyk, Francesco Dazzi, Baltasar Lucendo-Villarin, Jose Meseguer-Ripolles, James Williams, Giorgia Fanelli, David C. Hay, Fiona M. Watt, Giovanna Lombardi
Summary: Regenerative medicine aims to replace damaged tissues by stimulating tissue repair or transplanting cells. Pluripotent stem cells have the potential for unlimited cell production, making them valuable for regenerative medicine. However, immune responses pose a challenge to success. This study investigates the immunological properties of human pluripotent stem cell-derived hepatocyte-like cells and shows their potential for in vitro immunoregulation.
Article
Biophysics
Alice Pievani, Valentina Granata, Giacomo Desantis, Laura Antolini, Sara Ornaghi, Antonio Galleu, Andrea Biondi, Bernhard Gentner, Francesco Dazzi, Marta Serafini
Summary: Monocytes are effective at improving engraftment in HSPC transplantation, particularly in cases of limited cell doses such as CB transplantation and HSC gene therapy. Co-culturing monocytes increases survival and stemness of CB-CD34(+) cells. Soluble factors and direct-cell contact interactions play critical roles in the HSC-monocyte crosstalk.
BONE MARROW TRANSPLANTATION
(2022)
Article
Biochemistry & Molecular Biology
Jennifer Donau, Huan Luo, Iiris Virta, Annett Skupin, Margarita Pushina, Jana Loeffler, Frauke Haertel, Anupam Das, Thomas Kurth, Michael Gerlach, Dirk Lindemann, Peter S. Reinach, Stefan Mergler, Monika Valtink
Summary: TRPV4 overexpression altered the morphology of HCEC and reduced the dosage of GSK101 required to activate its channel activity.
Article
Biotechnology & Applied Microbiology
Sandhya Moise, Luigi Dolcetti, Francesco Dazzi, Paul Roach, Lee Buttery, Sheila MacNeil, Nick Medcalf
Summary: This study evaluated the immunosuppressive effect of alginate-encapsulated MSCs on proliferating mouse splenocytes. The results showed that MSCs significantly suppressed splenocyte proliferation via both direct and indirect mechanisms. Additionally, the study demonstrated for the first time that MSCs can sustain immunosuppressive activity when physically isolated from splenocytes.
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2022)
Review
Immunology
Chiara Giacomini, Cecilia Graneli, Ryan Hicks, Francesco Dazzi
Summary: Mesenchymal stromal cells (MSCs) have shown good safety but mixed efficacy in the treatment of various clinical conditions. The heterogeneity of cell preparations, lack of mechanistic understanding, and poorly defined criteria for patient stratification have hindered informative clinical trials. However, studies have shown that apoptotic MSCs can be phagocytosed by anti-inflammatory cells, suggesting that the activation state of cytotoxic T or NK cells can be used as a biomarker for predicting clinical responses to MSC treatment. MSCs can orchestrate inflammatory responses through different mechanisms, including cell-contact and inflammatory cytokine-mediated licensing.
CELLULAR & MOLECULAR IMMUNOLOGY
(2023)
Meeting Abstract
Surgery
Caterina Lonati, Carlotta Lista, Michele Battistin, Alessandra Bertacco, Daniele Dondossola, Margherita Carbonaro, Umberto Cillo, Francesco Dazzi
TRANSPLANT INTERNATIONAL
(2021)
Meeting Abstract
Oncology
James W. Opzoomer, Joanne E. Anstee, Isaac Dean, Emily J. Hill, Ihssane Bouybayoune, Jonathan Caron, Tamara Muliaditan, Peter Gordon, Rosamond Nuamah, Sarah E. Pinder, Tony Ng, Francesco Dazzi, Shahram Kordasti, David R. Withers, Toby Lawrence, James N. Arnold