Article
Chemistry, Multidisciplinary
Haibo Chen, Xuewei Dong, Luping Ou, Chiyun Ma, Bing Yuan, Kai Yang
Summary: In this study, the interaction between irradiated nanoparticles (NPs) and lipid bilayer membranes was investigated using molecular dynamics simulations. The results showed that the wrapping of membranes around NPs is thermally regulated and dependent on NP properties and lipid species. These findings offer valuable insights for understanding cellular internalization of NPs and developing strategies to modulate NP endocytosis.
Article
Physics, Fluids & Plasmas
Ke Xiao, Rui Ma, Chen-Xu Wu
Summary: The study investigates the theoretical model and phenomenon of active entry of self-propelled pathogens into host cells, revealing a wrapping transition triggered by active force. The results may offer guidance for research on the cellular entry of active particles driven by activity.
Article
Physics, Fluids & Plasmas
Yudie Zhang, Long Li, Jizeng Wang
Summary: The study explores the wrapping process of elastic vesicle NPs by a finite lipid membrane driven by receptor-ligand bonds. It finds that ligand density affects the morphology evolution in the wrapping state and optimal ligand density can achieve maximum wrapping degree. Experimental observations are consistent with model predictions.
Article
Engineering, Biomedical
Libing Fu, Bingyang Shi, Shihui Wen, Marco Morsch, Guoying Wang, Zhiguang Zhou, Chao Mi, Mohammad Sadraeian, Gungun Lin, Yiqing Lu, Dayong Jin, Roger Chung
Summary: This study successfully synthesized bright UCNPs with different aspect ratios using a seed-mediated approach and systematically evaluated the effects of physical aspect ratios and PEGylation on cellular uptake. The findings demonstrated that PEGylated UCNPs can enhance their stability and cellular uptake capacity, and an optimal aspect ratio for UCNP uptake was identified. These results provide crucial insights for optimizing UCNPs as drug carriers.
ACTA BIOMATERIALIA
(2022)
Article
Physics, Fluids & Plasmas
Ke Xiao, Rui Ma, Chen-Xu Wu
Summary: By using the Onsager variational principle, this study obtained a general wrapping equation for nonspherical self-propelled nanoparticles. Two analytical critical conditions were theoretically found, indicating a continuous full uptake for prolate particles and a snapthrough full uptake for oblate particles. Numerical calculations in phase diagrams accurately captured the critical boundaries of full uptake in terms of active force, aspect ratio, adhesion energy density, and membrane tension. The study found that enhancing activity, reducing viscosity, increasing adhesion energy density, and decreasing membrane tension can significantly improve the wrapping efficiency of the self-propelled nonspherical nanoparticles. These results provide a panoramic view of the uptake dynamics and offer guidance for designing effective active nanoparticle-based drug delivery vehicles.
Article
Chemistry, Multidisciplinary
Akkaranunt Supakijsilp, Jing He, Xubo Lin, Jian Ye
Summary: This study investigates the interaction between nanoparticles and the pulmonary surfactant monolayer, focusing on the effects of nanoparticle elasticity on translocation across the monolayer. Molecular dynamics simulations reveal that increasing stiffness enhances the penetrability of nanoparticles, while soft nanoparticles remain trapped within the monolayer. Active cellular uptake pathways exhibit longer penetration times, greater penetration distances, and more contacts compared to passive pathways, due to ligand-receptor interactions.
Article
Chemistry, Applied
Yemei Sun, Linlin Shang, Xiaoshuang Xia, Di Meng, Yun Ren, Jiaqi Zhang, Man Yao, Xinghua Zhou, Yun Wang
Summary: Chitosan treatment affected the transcriptome and physiological pathways of Penicillium expansum, with up-regulation of the gene encoding clathrin adaptor protein mu-subunit (PeCAM) involved in clathrin-dependent endocytosis. Deletion of PeCAM resulted in morphological changes in fungal cells and reduced sensitivity to chitosan, indicating that chitosan uptake is mediated by clathrin-dependent endocytosis and plays a crucial role in its antifungal activity.
CARBOHYDRATE POLYMERS
(2021)
Article
Biochemical Research Methods
Suman Pal, Ina F. de la Fuente, Shraddha S. Sawant, Jenna N. Cannata, Wu He, Jessica L. Rouge
Summary: In this study, the mechanisms and effects of serum on the uptake of DNA-functionalized micelles by cells were investigated. It was found that scavenger receptor-mediated, caveolae-dependent endocytosis is the major uptake pathway. Additionally, energy-independent pathways and clathrin-mediated endocytosis were involved in the uptake of particles degraded by enzymes. This study provides insights into the cytosolic delivery and therapeutic activity of DNA-functionalized nanomaterials, and highlights the stability of nucleic acids delivered by the micellular NAN platform in the presence of serum.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Patrick Knoll, Giuseppe Francesco Racaniello, Valentino Laquintana, Florina Veider, Ahmad Saleh, Anna Seybold, Nunzio Denora, Andreas Bernkop-Schnuerch
Summary: The aim of this study was to evaluate the uptake mechanism of thiolated nanostructured lipid carriers (NLCs). NLCs with different modifications were evaluated for size, surface morphology, stability, cytotoxicity, adhesion to the cell surface, internalization, and their influence on paracellular permeability. The results showed that NLCs with shorter PEG chains and thiolation exhibited higher cellular uptake. The cellular uptake of all NLCs was mainly clathrin-mediated endocytosis, and thiolated NLCs also showed caveolae-dependent and clathrin-and caveolae-independent uptake.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Agata Noelia Traverso, David Jose Fragale, Diego Luis Viale, Octavio Garate, Pablo Torres, Gaston Valverde, Alejandro Berra, Ana Vanesa Torbidoni, Juan Sebastian Yakisich, Mariano Grasselli, Martin Radrizzani
Summary: In this study, a two-step procedure for preparing biohybrid nanoparticles with a hydrophobic quantum dot core and a multilayer coating of human serum albumin is reported. These nanoparticles, decorated with proteins like human serum albumin or human transferrin, retained their fluorescent properties and showed no corona effect in the presence of serum. They were selectively taken up by lung cancer and neuroblastoma cells but not by non-cancerous cells or differentiated neurons. Moreover, digitoxin-loaded transferrin-decorated nanoparticles specifically reduced the number of lung cancer cells. Finally, the in vivo uptake of these biohybrid nanoparticles by retinal cells in mice demonstrated their excellent targeting and traceability.
Article
Nanoscience & Nanotechnology
Ankan Kumar Sarkar, Santanu Shaw, Himali Arora, Pankaj Seth, Nikhil R. R. Jana
Summary: Subcellular targeting of drugs can be enhanced by designing nanocarriers for direct cell membrane translocation, bypassing endosomal/lysosomal trafficking. This approach allows rapid and extensive delivery of drugs to the cell nucleus within a few minutes, without undergoing vesicular coating and lysosomal trafficking. This nonendocytic uptake method provides a potential strategy for nanocarrier-based subcellular targeting of drugs for more effective therapy.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Se-Na Kim, Chun Gwon Park, Chang Hee Min, Seung Ho Lee, Yun Young Lee, Na Kyeong Lee, Young Bin Choy
Summary: The shape of Fe-based MOF nanoparticles affects cellular uptake, with octahedral nanoparticles showing faster uptake and rod-shaped nanoparticles showing sustained uptake. This finding provides important information for the application of MOF nanoparticles as intracellular drug delivery carriers.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Zhiwei Liao, Hui Liu, Liang Ma, Jie Lei, Bide Tong, Gaocai Li, Wencan Ke, Kun Wang, Xiaobo Feng, Wenbin Hua, Shuai Li, Cao Yang
Summary: EVs deliver antioxidant proteins to protect against pyroptosis of nucleus pulposus cells; Cavin-2-modified engineering EVs exhibit an improved uptake rate in TNF-alpha-treated NPCs, ameliorating cell death and retarding IDD progression.
Article
Chemistry, Multidisciplinary
Mauro Sousa De Almeida, Patricia Taladriz-Blanco, Barbara Drasler, Sandor Balog, Phattadon Yajan, Alke Petri-Fink, Barbara Rothen-Rutishauser
Summary: The uptake of nanoparticles by cells can trigger gene expression regulation, specifically related to inflammation, chemotaxis, and cell adhesion. Understanding these cellular processes is important for the approval of new nanomedicines.
Article
Engineering, Geological
Langping Li, Hengxing Lan, Alexander Strom, Renato Macciotta
Summary: Aspect ratio is insufficient in characterizing the geometry of landslides. This paper introduces the concept of landslide longitudinal shape as a complement to the aspect ratio. The longitudinal shape is determined by the relative width change and fluctuation, and can provide additional information about landslides.