期刊
MOLECULAR MEDICINE REPORTS
卷 13, 期 5, 页码 4085-4094出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2016.5024
关键词
renal cell carcinoma; microRNA-30a-5p; polypeptide N-acetylgalactosaminyltransferase 7; tumor suppressor
资金
- National Natural Science Foundation of China [81101922]
- Guangdong Natural Science Foundation [2015A030313889]
- Science and Technology Development Fund Project of Shenzhen [JCYJ20120616144352139, JCYJ20130402114702124, JCYJ20140415162542975, JCYJ20150403091443329]
- Fund of Guangdong Key Medical Subject
Renal cell carcinoma (RCC) is the most common type of malignant tumor of the adult kidney and has a poor prognosis. MicroRNAs (miRs) are important in a wide range of biological and pathological processes, including cell differentiation, migration, growth, proliferation, apoptosis and metabolism. The present study aimed to determine the role exerted by miR-30a-5p in the tumorigenesis of RCC. The expression levels of miR-30a-5p in RCC tissues and RCC-derived cells were demonstrated to be significantly downregulated by real-time quantitative polymerase chain reaction (RT-qPCR). Wound scratch assay, cell proliferation assay and flow cytometric analysis revealed that the abilities of migration and proliferation of the RCC-derived cells were suppressed, whereas cell apoptosis was promoted, when miR-30a-5p was overexpressed in these cells. N-acetylgalactos aminyltransferase 7 (GALNT7) was predicted to be one target gene of miR-30a-5p by bioinformatics analysis. Luciferase reporter assay, RT-qPCR and western blotting were performed to confirm that GALNT7 is the direct conserved target of miR-30a-5p. These results suggested that miR-30a-5p has a tumor-suppressive role in the tumorigenesis of RCC.
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