期刊
MOLECULAR CELL
卷 64, 期 1, 页码 134-147出版社
CELL PRESS
DOI: 10.1016/j.molcel.2016.09.007
关键词
-
资金
- Wellcome Trust [100955]
- Medical Research Council (UK)
- Wellcome Trust [100955/Z/13/Z] Funding Source: Wellcome Trust
- MRC [MC_U120074328] Funding Source: UKRI
- Medical Research Council [MC_U120074328] Funding Source: researchfish
- Wellcome Trust [100955/Z/13/Z] Funding Source: researchfish
Sister chromatid intertwines (SCIs), or catenanes, are topological links between replicated chromatids that interfere with chromosome segregation. The formation of SCIs is thought to be a consequence of fork swiveling during DNA replication, and their removal is thought to occur because of the intrinsic feature of type II topoisomerases (Top2) to simplify DNA topology. Here, we report that SCIs are also formed independently of DNA replication during G(2)/M by Top2-dependent concatenation of cohesed chromatids due to their physical proximity. We demonstrate that, in contrast to G(2)/M, Top2 removes SCIs from cohesed chromatids at the anaphase onset. Importantly, SCI removal in anaphase requires condensin and coincides with the hyperactivation of condensin DNA supercoiling activity. This is consistent with the long-standing proposal that condensin provides a bias in Top2 function toward decatenation. A comprehensive model for the formation and resolution of toxic SCI entanglements on eukaryotic genomes is proposed.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据