Article
Immunology
Qiuyu Wu, Xiangjie Chen, Caixia Qiao, Xinhua Cao, Qian Du, Yukang Yuan, Yibo Zuo, Ying Miao, Zhijin Zheng, Tingting Zhang, Lichao Zang, Xinyu Yang, Weifeng Shi, Zhijun Xie, Yang Xu, Depei Wu, Chengping Wen, Hui Zheng
Summary: Methotrexate (MTX) is commonly used to treat rheumatoid arthritis, acute leukemia, and psoriasis. However, the exact mechanism of myelosuppression caused by MTX is still unknown. This study explores the impact of MTX on the Notch signaling pathway and finds that MTX strongly suppresses this pathway by stabilizing Numb proteins and reducing Notch1 protein levels. Interestingly, the monomeric drug Triptolide can alleviate the inhibitory effect of MTX on the Notch signaling pathway.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Oncology
Linmei Zheng, Jie Song, Rong Tang, Xiaoju Chen, Li Wang, Dongcai Wu, Hui Cen, Lei Shi
Summary: miR-524-5p expression levels are lower in patients with preeclampsia, and it regulates the proliferation and invasion of HTR-8/SVneo cells by targeting NUMB and affecting the Notch signaling pathway.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Entomology
Do-Hwan Lim, Min-Seok Choi, Ji Won Jeon, Young Sik Lee
Summary: The miR-252-5p-Rab6 regulatory axis controls the Notch signaling pathway in Drosophila wing development. The loss of miR-252 increases wing size, while its overexpression leads to patterning defects in adult wings. The overexpression-induced wing phenotypes are caused by aberrant Notch signaling and intracellular accumulation of full-length Notch receptor, possibly due to defects in intracellular trafficking and autophagy-mediated degradation.
Article
Cell Biology
Lianjie Miao, Yangyang Lu, Anika Nusrat, Hala Y. Abdelnasser, Sayantap Datta, Bin Zhou, Robert J. Schwartz, Mingfu Wu
Summary: This study found that NFPs regulate progenitor cell differentiation, cardiomyocyte proliferation, and trabecular morphogenesis partially through Notch1 during cardiac development. Deletion of Notch1 or RBPJk in the MDKO did not fully rescue structural defects, but partially rescued defects in cardiac progenitor cell differentiation, cardiomyocyte proliferation, and trabecular morphogenesis.
Article
Cell Biology
Thomas J. Krzystek, Joseph A. White, Rasika Rathnayake, Layne Thurston, Hayley Hoffmar-Glennon, Yichen Li, Shermali Gunawardena
Summary: This study reveals a novel role for HTT in the retrograde movement of a Rab7-LAMP1-containing signaling late endosome and identifies adaptors Hip1 and Rilpl as important factors in this process.
Article
Biochemistry & Molecular Biology
Shu-Ching Huang, Long V. Vu, Faye H. Yu, Dan T. Nguyen, Edward J. Benz
Summary: The unequal distribution of Numb plays a critical role in cell diversification during asymmetric cell division, which is regulated by spindle orientation. 4.1R functions as a member of the NuMA-LGN-dynein/dynactin complex and interacts with NuMA to regulate spindle orientation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biology
Francisca Bertin, Jorge Jara-Wilde, Benedikt Auer, Andres Koehler-Solis, Carolina Gonzalez-Silva, Ulrich Thomas, Jimena Sierralta
Summary: This study reveals that the endoplasmic reticulum (ER) and endosomes play a role in transporting proteins and lipids in motor neurons. In Drosophila, mutations in a protein called Atlastin (Atl) leads to synaptic defects, affecting the abundance and distribution of synaptic vesicles. Contrary to previous belief, the BMP signaling pathway is not involved in this process. The findings suggest that Atl modulates neurotransmitter release by altering the distribution of synaptic vesicles.
BIOLOGICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Jin Sun, Xia Wang, Rong-Gang Xu, Decai Mao, Da Shen, Xin Wang, Yuhao Qiu, Yuting Han, Xinyi Lu, Yutong Li, Qinyun Che, Li Zheng, Ping Peng, Xuan Kang, Ruibao Zhu, Yu Jia, Yinyin Wang, Lu-Ping Liu, Zhijie Chang, Jun-Yuan Ji, Zhao Wang, Qingfei Liu, Shao Li, Fang-Lin Sun, Jian-Quan Ni
Summary: The study identifies HP1c as an essential epigenetic regulator of gut homeostasis in Drosophila, demonstrating its role in suppressing Notch signaling by interacting with key components of the pathway. The depletion of HP1c in Drosophila can be rescued by expressing human HP1 gamma, indicating its functional similarity to HP1c in Drosophila.
Article
Multidisciplinary Sciences
Qingyang Li, Chao Zhang, Chuchu Zhang, Renjie Duan, Yongzhi Hua
Summary: In this study, the immune function of CG4968, which encodes a typical OTU-type Dub in Drosophila melanogaster, was explored. The results showed that CG4968 plays a positive regulatory role in the IMD pathway, but not the Toll pathway, in a manner dependent on the OTU domain. Mechanistically, CG4968 was found to be associated with Imd and restrict its K48-linked ubiquitination, thereby contributing to its turnover. This study uncovered a novel regulatory mechanism involving the K48-linked ubiquitination of Imd in Drosophila innate immunity.
Article
Biochemistry & Molecular Biology
Polash Kumar Biswas, Sang Rok Park, Jongyub An, Kyung Min Lim, Ahmed Abdal Dayem, Kwonwoo Song, Hye Yeon Choi, Yujin Choi, Kyoung Sik Park, Hyun Jin Shin, Aram Kim, Minchan Gil, Subbroto Kumar Saha, Ssang-Goo Cho
Summary: In this study, the expression of GPR50 in CSLC and breast cancer cell lines was evaluated, and its role in CSLC progression was investigated. Knockdown of GPR50 in CSLC resulted in decreased cancer properties and stemness. GPR50 was found to regulate NF-kB and NOTCH signaling pathways, as well as ADAM-17 activity, in CSLC. Overall, this study provided novel insights into GPR50-mediated regulation of the NF-kappa B-Notch signaling pathway in CSLC and its implications for treatment strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Qing Zhang, Ting Chen, Yun Zhang, Lingxia Lyu, Bohuan Zhang, Chengchen Huang, Xuhao Zhou, Yutao Wu, Zhoubin Li
Summary: The expression of miR-30c-5p was significantly decreased in atherosclerotic arteries, and it inhibited VSMC differentiation from adventitial Sca-1(+) progenitor cells through targeting OPG, suggesting a new therapeutic target for VSMC-associated diseases.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Arindam Ray, Kajal Kamat, Maneesha S. Inamdar
Summary: Mitochondria are dynamic organelles that play a crucial role in blood stem and progenitor cell biology. The protein Asrij/OCIAD1 regulates mitochondrial morphology, energy metabolism, and cell differentiation in both human embryonic stem cells and Drosophila hematopoiesis. Studies in both model systems suggest a conserved role for Asrij in linking mitochondrial dynamics and progenitor differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Genetics & Heredity
Aresh Sahu, Susnata Karmakar, Sudipta Halder, Gaurab Ghosh, Sayan Acharjee, Purbasa Dasgupta, Ritabrata Ghosh, Girish Deshpande, Mohit Prasad
Summary: The study demonstrates the involvement of Gap Junction proteins Innexin2 and Innexin4 in the shape transition process of follicle cells in Drosophila ovaries. Calcium fluxes and STAT activation play crucial roles in modulating the shape transformation from cuboidal to squamous fate. The findings provide insights into the sequential molecular events and the contribution of neighboring cells in morphogenetic processes.
Article
Cell Biology
Dipayan De, Suvendra N. Bhattacharyya
Summary: Exposure to amyloid-beta oligomers leads to an increase in proinflammatory cytokines expression in glial cells, despite the presence of repressive microRNAs. The differential compartmentalization of repressive microRNAs in glial cells exposed to A beta(1-)(42) explains the aberrant microRNA function. Amyloid-beta oligomers affect the lysosomal targeting and recycling of miRNPs by masking Rab7a proteins on endosomal surfaces.
JOURNAL OF CELL SCIENCE
(2021)
Article
Cell Biology
Qi-Xin Wen, Biao Luo, Xiao-Yong Xie, Gui-Feng Zhou, Jian Chen, Li Song, Yue Liu, Shi-Qi Xie, Long Chen, Kun-Yi Li, Xiao-Jiao Xiang, Guo-Jun Chen
Summary: AP2S1 plays a crucial role in the pathophysiology of Alzheimer disease (AD) by regulating the degradation and translocation of APP, which affects the generation of A beta. Knockdown or overexpression of AP2S1 decreases or increases the protein levels of APP and A beta, respectively, suggesting its importance in the pathogenesis of AD. This regulation is achieved through lysosomal degradation rather than endocytosis.