Article
Cell Biology
Nan Qin, Zhixi Chen, Renhao Xue
Summary: Exocytosis of large dense core vesicles is crucial for hormone secretion in neuroendocrine cells. This study develops a novel model based on single vesicle release events to describe the architecture of the releasable vesicle pool and elucidate the heterogeneity of the vesicle priming mechanism.
Article
Neurosciences
Hui-Ju Yang, Pin-Chun Chen, Chien-Ting Huang, Tzu-Lin Cheng, Sheng-Ping Hsu, Chien-Yu Chen, Juu-Chin Lu, Chih-Tien Wang
Summary: The study demonstrated that Syn Ia regulates DCV exocytosis through phosphorylation, with a decrease in secretion rate observed when phosphodeficient Syn Ia is overexpressed. Additionally, Syn Ia may modulate the fusion pore dynamics of DCVs via specific phosphorylation sites, potentially interacting with proteins involved in fusion pore regulation. Immunoprecipitation and proximity ligation assays confirmed the phosphorylation-dependent interaction of Syn Ia with proteins like Syp on DCVs, highlighting a phosphorylation-mediated regulatory mechanism.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Biology
Bassam Tawfik, Joana S. Martins, Sebastien Houy, Cordelia Imig, Paulo S. Pinheiro, Sonja M. Wojcik, Nils Brose, Benjamin H. Cooper, Jakob Balslev Sorensen
Summary: Synaptotagmin-1 and synaptotagmin-7 act as fast and slow calcium sensors independently in vesicle fusion, with non-overlapping distributions when coexpressed. Synaptotagmin-7 promotes vesicle priming and inhibits depriming, affecting the fusion mediated by synaptotagmin-1. Both synergistic and competitive interactions are observed between synaptotagmin-1 and synaptotagmin-7.
Article
Biology
Sebastien Houy, Joana S. Martins, Noa Lipstein, Jakob Balslev Sorensen
Summary: This study investigated the relationship between phorbolesters and Munc13-1 and ubMunc13-2, and found that phorbolesters only stimulated secretion when ubMunc13-2 was dominant. The results showed that DAG/phorbolesters, ubMunc13-2, and Syt7 are important factors for stimulating dense-core vesicle priming.
Article
Multidisciplinary Sciences
Pengcheng Zhang, David Rumschitzki, Robert H. Edwards
Summary: This study investigates the release of secretory vesicles in mouse adrenal chromaffin cells and discovers diverse modes of release. Through comprehensive analysis, it is revealed that the variations in release cannot be solely explained by stochasticity, but involve distinct mechanisms. Furthermore, the loss of calcium sensor synaptotagmin 7 increases the proportion of slow events without altering the intrinsic properties of either class, suggesting the potential for independent regulation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Multidisciplinary
Xiulan He, Andrew G. Ewing
Summary: In the presence of chaotropic anions, the opening of nanometer biological vesicles becomes a two-step process, allowing for independent counting of molecules in each subvesicular compartment. The study revealed a 68% catecholamine binding to the dense-core and differentiated two populations of large dense-core vesicles, with differences in biogenesis speculated to be caused by an increase in electrostatic attraction.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Multidisciplinary Sciences
Bijeta Prasai, Gideon J. Haber, Marie-Paule Strub, Regina Ahn, John A. Ciemniecki, Kem A. Sochacki, Justin W. Taraska
Summary: By utilizing correlative super-resolution light and electron microscopy, researchers have mapped the location and organization of Rab-GTPases and their effectors on exocytic vesicles, shedding light on their functional roles within cells.
NATURE COMMUNICATIONS
(2021)
Article
Environmental Sciences
Xiaofei Yang, Rencan Nie, Gucheng Zhang, Luping Chen, He Li
Summary: Pansharpening is a technology that fuses a low spatial resolution MS image with its associated high spatial full resolution PAN image. We propose a novel multistage Dense-Parallel attention fusion network (DPAFNet), which utilizes parallel attention residual dense block (PARDB) modules and multistage feature fusion to better focus on and exploit the intrinsic features and correlation between images, resulting in superior fusion results.
Article
Chemistry, Multidisciplinary
Zhaohan Lin, Yinglin Li, Yuqi Hang, Changhe Wang, Bing Liu, Jie Li, Lili Yin, Xiaohan Jiang, Xingyu Du, Zhongjun Qiao, Feipeng Zhu, Zhe Zhang, Quanfeng Zhang, Zhuan Zhou
Summary: This study reveals that secretogranin II (SgII), a vesicle matrix protein, plays a role in regulating the size of large dense-core vesicles (LDCVs) through its liquid-liquid phase separation. SgII undergoes pH-dependent polymerization and forms droplets with Ca2+. These droplets recruit lipids similar to LDCV biogenesis. Knockdown of SgII leads to a decrease in neurotransmitter release by affecting LDCV size.
Article
Neurosciences
A. D. Roseborough, Y. Zhu, L. Zhao, S. R. Laviolette, S. H. Pasternak, S. N. Whitehead
Summary: The brain's response to acute injury involves increased permeability of the blood-brain barrier (BBB) and increased inflammation in microglia, which can lead to poor cognitive outcomes and neurological disease. Fibrinogen, a serum protein, enters the brain through the damaged BBB and interacts with local cells in a harmful way. Microglia, in response to injury, demonstrate increased activity of the NLRP3 inflammasome and release more pro-inflammatory cytokines. This study investigates the interaction between fibrinogen, microglial NLRP3 signaling, and extracellular vesicles (EVs), which can propagate inflammatory signaling and be detected in the circulation following BBB disruption.
NEUROBIOLOGY OF DISEASE
(2023)
Article
Chemistry, Multidisciplinary
Liang Cui, Ruixiao Peng, Chaofei Zeng, Jialu Zhang, Yinzhu Lu, Lin Zhu, Mengjiao Huang, Qinghua Tian, Yanling Song, Chaoyong Yang
Summary: In this study, a general strategy for in situ detection of tumor-derived extracellular vesicle miRNAs was developed using aptamer-mediated selective fusion. This method offers high selectivity, rapidity, and general applicability, and enables sensitive and selective detection of tumor-derived EV miRNAs using conventional flow cytometry without the need for lysis.
Article
Biology
Wei Zhou, Weiqi Bai, Jianhang Ji, Yugen Yi, Ningyi Zhang, Wei Cui
Summary: Steady progress has been made in deep learning-based blood vessel segmentation, but challenges such as inadequate sample sizes, neglect of contextual information, and loss of microvascular details still limit its progress. To address these limitations, a dual-path deep learning framework called MCDAU-Net is proposed, which divides fundus images into concentric patches with different scales and uses a Multi-scale Context Dense Aggregation Network to accurately extract blood vessel boundaries.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Education, Scientific Disciplines
Tanner J. Mitton, Ryan Elder, Jacob B. Hunter
Summary: Concerns regarding interview hoarding in the Match process have led to calls for a cap on the number of interviews an applicant can accept. However, no study has examined the effects of interview caps on applicants or a specialty. The authors conducted a simulation study of otolaryngology Match and found that interview caps can lead to more equal distribution of interviews among applicants, but also significantly reduce the probability of matching for more competitive applicants.
Article
Computer Science, Artificial Intelligence
Zhi Chang, Dexin Zhao, Huilin Chen, Jingdan Li, Pengfei Liu
Summary: The study aims to generate event proposals using visual-audio cues, enhance event-level representations, capture relationships between events, and fuse multi-modal information through an attention-gating mechanism, proposing an event-centric multi-modal fusion approach for dense video captioning. Experimental results demonstrate significant progress on the ActivityNet Caption and YouCook2 datasets.
Article
Geography, Physical
Can Pu, Chuanyu Yang, Jinnian Pu, Radim Tylecek, Robert B. Fisher
Summary: This study proposes a solution based on a newly-designed panoramic stereo camera and a hybrid software framework to address the importance of recovering an outdoor environment's surface mesh for an agricultural robot during task planning and remote visualization. The solution includes three fusion modules: disparity fusion, pose fusion, and volumetric fusion. The proposed framework and its three fusion modules demonstrate superior performance in experiments.
ISPRS JOURNAL OF PHOTOGRAMMETRY AND REMOTE SENSING
(2023)
Article
Cell Biology
Xingmin Zhang, Shan Jiang, Kelly A. Mitok, Lingjun Li, Alan D. Attie, Thomas F. J. Martin
JOURNAL OF CELL BIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Sebastian Koehler, Leigh Carmody, Nicole Vasilevsky, Julius O. B. Jacobsen, Daniel Danis, Jean-Philippe Gourdine, Michael Gargano, Nomi L. Harris, Nicolas Matentzoglu, Julie A. McMurry, David Osumi-Sutherland, Valentina Cipriani, James P. Balhoff, Tom Conlin, Hannah Blau, Gareth Baynam, Richard Palmer, Dylan Gratian, Hugh Dawkins, Michael Segal, Anna C. Jansen, Ahmed Muaz, Willie H. Chang, Jenna Bergerson, Stanley J. F. Laulederkind, Zafer Yueksel, Sergi Beltran, Alexandra F. Freeman, Panagiotis I. Sergouniotis, Daniel Durkin, Andrea L. Storm, Marc Hanauer, Michael Brudno, Susan M. Bello, Murat Sincan, Kayli Rageth, Matthew T. Wheeler, Renske Oegema, Halima Lourghi, Maria G. Della Rocca, Rachel Thompson, Francisco Castellanos, James Priest, Charlotte Cunningham-Rundles, Ayushi Hegde, Ruth C. Lovering, Catherine Hajek, Annie Olry, Luigi Notarangelo, Morgan Similuk, Xingmin A. Zhang, David Gomez-Andres, Hanns Lochmueller, Helene Dollfus, Sergio Rosenzweig, Shruti Marwaha, Ana Rath, Kathleen Sullivan, Cynthia Smith, Joshua D. Milner, Dorothee Leroux, Cornelius F. Boerkoel, Amy Klion, Melody C. Carter, Tudor Groza, Damian Smedley, Melissa A. Haendel, Chris Mungall, Peter N. Robinson
NUCLEIC ACIDS RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Kent A. Shefchek, Nomi L. Harris, Michael Gargano, Nicolas Matentzoglu, Deepak Unni, Matthew Brush, Daniel Keith, Tom Conlin, Nicole Vasilevsky, Xingmin Aaron Zhang, James P. Balhoff, Larry Babb, Susan M. Bello, Hannah Blau, Yvonne Bradford, Seth Carbon, Leigh Carmody, Lauren E. Chan, Valentina Cipriani, Alayne Cuzick, Maria Della Rocca, Nathan Dunn, Shahim Essaid, Petra Fey, Chris Grove, Jean-Phillipe Gourdine, Ada Hamosh, Midori Harris, Ingo Helbig, Maureen Hoatlin, Marcin Joachimiak, Simon Jupp, Kenneth B. Lett, Suzanna E. Lewis, Craig McNamara, Zoe M. Pendlington, Clare Pilgrim, Tim Putman, Vida Ravanmehr, Justin Reese, Erin Riggs, Sofia Robb, Paola Roncaglia, James Seager, Erik Segerdell, Morgan Similuk, Andrea L. Storm, Courtney Thaxon, Anne Thessen, Julius O. B. Jacobsen, Julie A. McMurry, Tudor Groza, Sebastian Koehler, Damian Smedley, Peter N. Robinson, Christopher J. Mungall, Melissa A. Haendel, Monica C. Munoz-Torres, David Osumi-Sutherland
NUCLEIC ACIDS RESEARCH
(2020)
Article
Health Care Sciences & Services
Xingmin Aaron Zhang, Amy Yates, Nicole Vasilevsky, J. P. Gourdine, Tiffany J. Callahan, Leigh C. Carmody, Daniel Danis, Marcin P. Joachimiak, Vida Ravanmehr, Emily R. Pfaff, James Champion, Kimberly Robasky, Hao Xu, Karamarie Fecho, Nephi A. Walton, Richard L. Zhu, Justin Ramsdill, Christopher J. Mungall, Sebastian Koehler, Melissa A. Haendel, Clement J. McDonald, Daniel J. Vreeman, David B. Peden, Tellen D. Bennett, James A. Feinstein, Blake Martin, Adrianne L. Stefanski, Lawrence E. Hunter, Christopher G. Chute, Peter N. Robinson
NPJ DIGITAL MEDICINE
(2019)
Article
Biology
Xingmin Aaron Zhang, Thomas F. J. Martin
