Article
Genetics & Heredity
Ji-Eun Jeong, Binna Seol, Han-Seop Kim, Jae-Yun Kim, Yee-Sook Cho
Summary: This study revealed distinct alternative splicing events in iMSCs compared to UCB-MSCs, with skipped exons being the predominant type of AS found in iMSCs. Differentially spliced genes were mainly enriched in functions associated with focal adhesion, extracellular exosomes, and other cellular processes. Identified splice isoforms of selected genes were further validated by RT-PCR analysis.
Review
Engineering, Biomedical
Jay R. K. Samal, Vignesh K. Rangasami, Sumanta Samanta, Oommen P. Varghese, Oommen P. Oommen
Summary: Studies have shown that culturing MSCs in a low oxygen environment can upregulate stem cell markers and promote cell proliferation in an undifferentiated state. However, there is ambiguity in the results due to differences in oxygen gradients and culture conditions among different studies. This progress report focuses on conflicting findings related to the application of hypoxic conditions for improving MSC proliferation or differentiation, aiming to decipher the factors influencing MSC characteristics under hypoxia.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Cell & Tissue Engineering
Guanyin Chen, Dong Fan, Wangqian Zhang, Shuning Wang, Jintao Gu, Yuan Gao, Lei He, Weina Li, Cun Zhang, Meng Li, Yingqi Zhang, Zhaohui Liu, Qiang Hao
Summary: This study found that under hypoxic conditions, there was a significant increase in the expression of Mkx in MSCs. Mkx mediated hypoxia-induced tenogenic differentiation of MSCs but could not completely repress the proliferation of hypoxic MSCs.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Qi Chen, Meng Gu, Zhi-kang Cai, Hu Zhao, Shi-cheng Sun, Chong Liu, Ming Zhan, Yan-bo Chen, Zhong Wang
Summary: CD44 serves as a marker of cancer stem cells in many tumors, with alternative splicing generating various isoforms. This study reveals a novel mechanism where TGF-beta 1 induces CD44 alternative splicing, promoting epithelial-to-mesenchymal transition and stemness in prostate cancer cells. The findings demonstrate the important roles of CD44 isoforms and TGF-beta 1 in tumor progression.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Nutrition & Dietetics
Song Hee Lee, Bo Young Choi, A. Ra Kho, Dae Ki Hong, Beom Seok Kang, Min Kyu Park, Si Hyun Lee, Hui Chul Choi, Hong Ki Song, Sang Won Suh
Summary: The combined treatment of pyruvate and DCA shows protective effects against seizure-induced hippocampal neuronal cell death by increasing the proportion of pyruvate converted to ATP.
Article
Chemistry, Medicinal
Shengying Lou, Ting Huang, Jifeng Qi, Tianyuan Zhang, Jianqing Gao, Sunliang Cui
Summary: The lack of suitable stem cells is currently a limiting factor in the success of stem cell therapy for neurodegenerative diseases, but utilizing mesenchymal stem cells shows potential for treating these diseases. Enhancing the neuronal differentiation capacity of MSCs through bioengineering with small molecules is a promising strategy.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Biotechnology & Applied Microbiology
Sonali Rawat, Krishan Gopal Jain, Deepika Gupta, Pawan Kumar Raghav, Rituparna Chaudhuri, Pinky, Adeeba Shakeel, Varun Arora, Harshita Sharma, Debika Debnath, Ankarao Kalluri, Ashwini K. Agrawal, Manjeet Jassal, Amit K. Dinda, Prabir Patra, Sujata Mohanty
Summary: The study successfully differentiated mesenchymal stem cells into dopaminergic neurons using PCL-G nanocomposites, showing promising potential. The newly differentiated neurons exhibited unique features, including enhanced neuronal arborization, intracellular Ca2+ influx, and dopamine secretion.
Article
Oncology
Laurynas Vilys, Inga Peciuliene, Egle Jakubauskiene, Ruta Zinkeviciute, Yuichi Makino, Arvydas Kanopka
Summary: The splicing machinery is crucial for cellular adaptability and hypoxia can alter RNA splicing, leading to the formation of different Fas mRNA isoforms.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Nidaa A. Ababneh, Ban Al-Kurdi, Fatima Jamali, Abdalla Awidi
Summary: This study compared the differentiation abilities of MSCs isolated from different human tissue sources, and found that WJ-MSCs had a higher potential to differentiate into NSC and dopaminergic-like cells. This could provide an additional or alternative source of cells for neurological cell-based therapies.
Article
Clinical Neurology
Wujun Zhao, Miaomiao Li, Shuai Wang, Zhuang Li, Han Li, Shaoyi Li
Summary: This study reveals the regulatory role of circSRRM4 in epilepsy and its correlation with the expression of PKM2 and the regulation of SRSF3. Silencing circSRRM4 can reduce the incidence and frequency of epilepsy and improve related pathological changes.
NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Hyoungki Kim, Soonjo Kwon
Summary: The study demonstrates that a hypoxic environment, created using specific chemicals in a suspension culture, enhances the proliferation of mouse clonal mesenchymal stem cells and promotes their differentiation into osteogenic cells.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Shangbiao Li, Yanwen Chen, Yuxin Xie, Hongchao Zhan, Yu Zeng, Kunlin Zeng, Li Wang, Ziling Zhan, Cuiying Li, Liqian Zhao, Xiaoxia Chen, Yujing Tan, Zhongyong Wang, Junguo Bu, Ye Song, Fan Deng, Aidong Zhou
Summary: The study reveals that FBXO7 confers mesenchymal properties and chemoresistance in glioblastoma. FBXO7 upregulation in mesenchymal glioblastoma stabilizes Rbfox2 through K63-linked ubiquitination after its arginine dimethylation by PRMT5, and thus controls Rbfox2-mediated splicing of mesenchymal genes, resulting in glioblastoma stem cell self-renewal, mesenchymal transformation, and chemoresistance. Targeting the FBXO7-Rbfox2 axis represents a potential strategy for glioblastoma treatment.
Review
Biochemistry & Molecular Biology
Subhashis Natua, Cheemala Ashok, Sanjeev Shukla
Summary: Maintenance of oxygen homeostasis is crucial for the existence of multicellular organisms, and disruption of this homeostasis can lead to pathological hypoxia in various conditions. Cellular adaptations include changes in gene expression, post-transcriptional modification of gene products, bioenergetics, and metabolism under hypoxic conditions. Hypoxia-induced alternative splicing events are associated with diseases like cancer, cardiovascular diseases, and neurological disorders, offering potential strategies for novel translational diagnosis and therapeutic interventions.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Rui Cheng, Lixing Xiao, Wenyang Zhou, Xiyun Jin, Zhaochun Xu, Chang Xu, Pingping Wang, Meng Luo, Mengyun Wang, Kexin Ma, Huimin Cao, Yan Huang, Xiaoyu Lin, Fenglan Pang, Yiqun Li, Qinghua Jiang
Summary: This study conducted a pan-cancer analysis of alternative splicing of splicing factors and identified 167 splicing factors with implications in regulating cancer-specific splicing patterns. The findings suggest that alternative splicing of splicing factors may serve as common regulators for alternative splicing in different cancers, highlighting its potential importance in tumorigenesis. Additionally, a splicing-derived neoepitopes database (ASPNs) was developed to provide putative alternative splicing-derived neoepitopes of 16 cancer types.
Article
Environmental Sciences
Yuhang Ren, Yuan Tian, Xuebin Mao, Haishen Wen, Xin Qi, Jinku Li, Jifang Li, Yun Li
Summary: This study investigated the gene expression profiles and alternative splicing events in the spotted sea bass fish under hypoxia stress. The results revealed the activation of the HIF signal network system and the suppression of the cell cycle process in response to hypoxia. Additionally, key biological pathways and alternative splicing events associated with hypoxia were identified. These findings contribute to a better understanding of the molecular mechanisms of hypoxia response in spotted sea bass and other fish species.
FRONTIERS IN MARINE SCIENCE
(2022)