Article
Microbiology
Marina Pascual-Ortiz, Eva Walla, Ursula Fleig, Adolfo Saiardi
Summary: This study investigated the regulation of polyP metabolism in yeast, revealing that different yeasts utilize different classes of IPPs to regulate polyP synthesis. While the mechanism of polyP synthesis is conserved in yeasts, the regulatory players are diverse.
Article
Microbiology
Bradley Benjamin, Yehuda Goldgur, Nikolaus Jork, Henning J. J. Jessen, Beate Schwer, Stewart Shuman
Summary: The fission yeast Schizosaccharomyces pombe phosphate regulon is sensitive to the level of the inositol pyrophosphate signaling molecule 1,5-IP8. The enzyme Asp1, consisting of a kinase domain and a pyrophosphatase domain, plays a crucial role in determining the dynamics of IP8. Crystal structures of the Asp1 kinase domain reveal its conformational changes upon ligand binding and its substrate specificity. Wild-type Asp1 kinase can utilize N-6-benzyl-ATP as a phosphate donor.
Article
Microbiology
Natascha Andrea Kuenzel, Abel R. Alcazar-Roman, Adolfo Saiardi, Simon M. Bartsch, Sarune Daunaraviciute, Dorothea Fiedler, Ursula Fleig
Summary: Inositol pyrophosphates (IPPs) are a class of signaling molecules that regulate central biological processes in eukaryotes. This study demonstrates that IP8 levels control chromosome transmission fidelity by modulating spindle function and kinetochore architecture.
Article
Biochemistry & Molecular Biology
Kim Kiat Lim, Hwei Yee Teo, Yuan Yee Tan, Yi Bing Zeng, Ulysses Tsz Fung Lam, Mahesh Choolani, Ee Sin Chen
Summary: MTHFR is a key enzyme in the folate metabolic pathway, linked to various human conditions. The fission yeast gene met11(+) shows similar function to MTHFR, affecting chromosome stability and meiotic fidelity. Cooperation among Met11, Rec8, and Sgo1 is crucial for proper genetic inheritance during mitosis and meiosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Hannah Rosenbach, Eva Walla, George E. Cutsail, James A. Birrell, Marina Pascual-Ortiz, Serena DeBeer, Ursula Fleig, Ingrid Span
Summary: The Asp1 protein is a bifunctional kinase/pyrophosphatase, containing an Fe-S cluster in vivo that does not influence its pyrophosphatase activity.
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2021)
Article
Microbiology
Ana M. Sanchez, Beate Schwer, Nikolaus Jork, Henning J. Jessen, Stewart Shuman
Summary: Inositol pyrophosphate 1,5-IP8 is a signaling molecule that regulates phosphate and polyphosphate homeostasis. Siw14 is identified as a pyrophosphatase involved in inositol pyrophosphate catabolism. Siw14 and Aps1 pyrophosphatases have redundant functions in fission yeast, and high levels of 1,5-IP8 are toxic for the cell.
Article
Cell Biology
Alberto Pineda-Santaella, Nazaret Fernandez-Castillo, Alberto Jimenez-Martin, Maria del Carmen Macias-Cabeza, Angela Sanchez-Gomez, Alfonso Fernandez-Alvarez
Summary: Chromosome segregation in female meiosis in many metazoans is mediated by acentrosomal spindles, which self-assemble without the involvement of centrosomes. A recent study in fungi demonstrated the formation of self-assembled microtubule arrays capable of segregating chromosomes, shedding light on the molecular mechanisms behind acentrosomal meiosis. Comparing self-assembled spindles with canonical SPB-dependent spindles revealed both similarities and differences, with potential for further understanding gametogenesis.
JOURNAL OF CELL SCIENCE
(2021)
Article
Cell Biology
Qian Zhu, Zhaodi Jiang, Xiangwei He
Summary: This study reveals that fusion of spindle pole bodies (SPBs) in polyploid zygotes of the fission yeast is blocked, leading to the inability of cell proliferation through mitosis. Consequently, these polyploid zygotes generate supernumerary SPBs in subsequent meiosis, resulting in multipolar nuclear divisions and the production of extra spores. The persistent localization of Pcp1 protein on SPBs is the cause of SPB fusion blockage, which is crucial for sexual reproduction and ploidy homeostasis in the fission yeast.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Juyoung Kim, Gohta Goshima
Summary: Mitosis is a fundamental process in eukaryotes, and its essential genes can be bypassed through genetic or environmental changes. This study discovered the bypass of essentiality of a mitosis regulator, Polo-like kinase, through specific mutations and down-regulation of glucose uptake. The study also identified casein kinase I as an alternative mechanism for microtubule nucleation. The findings have implications for understanding mitosis and selecting chemotherapeutic compounds.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Cell Biology
Maya I. Anjur-Dietrich, Colm P. Kelleher, Daniel J. Needleman
Summary: Chromosome segregation, driven by the spindle, is a crucial process in cell division. Extensive research on spindles in different systems over the past 150 years has revealed commonalities and contrasts. Understanding the sites of force generation and fundamental mechanical principles of chromosome segregation is essential, as conserved sites of force generation may interact differently in different spindles, leading to distinct mechanical mechanisms. Combining biophysical experiments, coarse-grained theories, and evolutionary genetics can enhance our understanding of chromosome segregation in the future.
Article
Cell Biology
Shane Chen, Maria Lyanguzova, Ross Kaufhold, Karen M. Plevock Haase, Hangnoh Lee, Alexei Arnaoutov, Mary Dasso
Summary: The localization of RanGAP to the nuclear envelope is widely conserved in multicellular organisms, but the specific targeting mechanisms vary. In Drosophila, it was found that RanGAP requires binding to a specific nucleoporin for its function and plays a crucial role in pupal development.
Review
Cell Biology
Angela R. R. Bunning, Mohan L. L. Gupta Jr.
Summary: Accurate chromosome segregation is essential for cell and organismal viability. The mitotic spindle, composed mainly of dynamic microtubules, plays a crucial role in this process by forming a bipolar configuration and generating tension across sister kinetochores. Recent advances have shed light on the role of tension as a biophysical signal that ensures accurate chromosome segregation by destabilizing aberrant kinetochore-microtubule attachments and reinforcing correct connections.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Maria Angeles Marquez-Monino, Raquel Ortega-Garcia, Megan L. Shipton, Elsa Franco-Echevarria, Andrew M. Riley, Julia Sanz-Aparicio, Barry V. L. Potter, Beatriz Gonzalez
Summary: DDP1 is a Nudix enzyme that exhibits activity on various substrates, with the molecular basis for its activity and different substrate binding modes revealed in this study. The findings have implications for inositide research and inhibitor design.
Review
Cell Biology
Masamitsu Sato, Yasutaka Kakui, Mika Toya
Summary: Meiosis is a specialized cell division process for gamete production in eukaryotes, with chromosomes and microtubules mutually influencing each other. Studies on the fission yeast Schizosaccharomyces pombe have revealed the organization of chromosomes, cytoskeleton, and cell cycle progression in mitosis and meiosis. Meiosis is strategically designed for faithful genetic material segregation and genetic diversity production through meiosis-specific and general factors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Soumitra Polley, Helen Mueschenborn, Melina Terbeck, Anna De Antoni, Ingrid R. Vetter, Marileen Dogterom, Andrea Musacchio, Vladimir A. Volkov, Pim J. Huis In 't Veld
Summary: During cell division, the Ndc80 complex plays a crucial role in linking chromosomes to spindle microtubules. This study demonstrates that the Ndc80 loop promotes direct interactions between adjacent Ndc80 complexes, which are necessary for stable end-on kinetochore-microtubule attachment and spindle assembly checkpoint satisfaction. Mutations in the loop impair these interactions and lead to cell arrest in mitosis.
Article
Biochemistry & Molecular Biology
Marina Pascual-Ortiz, Adolfo Saiardi, Eva Walla, Visnja Jakopec, Natascha A. Kuenzel, Ingrid Span, Anand Vangala, Ursula Fleig
MOLECULAR AND CELLULAR BIOLOGY
(2018)
Article
Multidisciplinary Sciences
Stefanie Teschler, Marek Bartkuhn, Natascha Kuenzel, Christian Schmidt, Steffen Kiehl, Gerhard Dammann, Reinhard Dammann
Article
Microbiology
Natascha Andrea Kuenzel, Abel R. Alcazar-Roman, Adolfo Saiardi, Simon M. Bartsch, Sarune Daunaraviciute, Dorothea Fiedler, Ursula Fleig
Summary: Inositol pyrophosphates (IPPs) are a class of signaling molecules that regulate central biological processes in eukaryotes. This study demonstrates that IP8 levels control chromosome transmission fidelity by modulating spindle function and kinetochore architecture.