Article
Biotechnology & Applied Microbiology
Chunyan Zhao, Xiaoteng Cui, Yan Zhao, Baoxin Qian, Nan Zhang, Lingbiao Xin, Chuanbo Ha, Jie Yang, Xinting Wang, Xingjie Gao
Summary: The study aimed to explore the potential impact of hepatocyte-specific SND1 deletion on insulin-resistant mice, but found that the absence of hepatic SND1 did not significantly alter the phenotypes of hepatic insulin resistance and acute liver failure. Enrichment analysis indicated SND1's involvement in regulating RNA or lipid metabolism, rather than glucose homeostasis or hepatic failure.
Article
Biochemistry & Molecular Biology
Caroline C. Duwaerts, Kevin Siao, Russell K. Soon, Chris Her, Takao Iwawaki, Kenji Kohno, Aras N. Mattis, Jacquelyn J. Maher
Summary: The transcription factor X-box binding protein-1 (XBP1) plays a central role in controlling cellular responses to endoplasmic reticulum stress. Genetic deletion of XBP1 in adult mouse liver led to acute liver injury in response to fructose challenge, primarily due to hyperactivation of IRE1 alpha. Further exploration of IRE1 alpha as a contributor to liver diseases is supported by these findings.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Biochemistry & Molecular Biology
Yuguo Yi, Weigao Zhang, Liang Tao, Qianchao Shao, Qian Xu, Yuxin Chen, Haibing Zhang, Jianfa Zhang, Dan Weng
Summary: This study investigated the role of RIP1 kinase in APAP-induced acute liver injury through genetic or pharmacological inhibition, providing evidence that RIP1 kinase activity plays an important role in the pathogenesis of APAP-induced liver injury. The results demonstrated that RIP1 kinase inactivation significantly attenuated APAP-induced liver injury and mortality, and that Nec-1, a RIP1 kinase inhibitor formulated with PEG400, could efficiently alleviate hepatotoxicity induced by APAP.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Medicine, Research & Experimental
Min-Hyun Kim, Yuan Li, Qiantao Zheng, Lin Jiang, Martin G. Myers Junior, Wen-Shu Wu, Liangyou Rui
Summary: A neuronal Slug/epigenetic reprogramming/leptin resistance axis was discovered, which promotes energy imbalance, obesity, and metabolic disease. Slug represses LepRb expression by binding to and inhibiting LepRb promoter, leading to obesity and metabolic disorders.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Biochemistry & Molecular Biology
Yang Yang, Huali Zhai, Yue Wan, Xiaofang Wang, Hui Chen, Lihou Dong, Taoyun Liu, Guifang Dou, Chutse Wu, Miao Yu
Summary: This study evaluated the effects of pharmaceutical-grade recombinant human HPS on acute liver injury in mice and monkeys. The results showed that HPS treatment significantly reduced hepatocyte death and inflammation, attenuated the development of acute liver failure, and promoted hepatocyte proliferation while preventing apoptosis and oxidative stress. The study suggested the potential of HPS as a novel drug for the treatment of acute liver injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Yukina Takeichi, Takashi Miyazawa, Shohei Sakamoto, Yuki Hanada, Lixiang Wang, Kazuhito Gotoh, Keiichiro Uchida, Shunsuke Katsuhara, Ryuichi Sakamoto, Takaya Ishihara, Keiji Masuda, Naotada Ishihara, Masatoshi Nomura, Yoshihiro Ogawa
Summary: The study demonstrates that deletion of MFF in liver induces ER stress and reduces hepatic TG secretion both in vivo and in vitro. MffLiKO mice are more susceptible to HFD-induced NASH phenotype due to ER stress-induced apoptosis of hepatocytes and suppression of TG secretion. This study provides evidence for the involvement of mitochondrial fission in the development of NASH.
Article
Cardiac & Cardiovascular Systems
Leah B. Kosyakovsky, Peter C. Austin, Heather J. Ross, Xuesong Wang, Husam Abdel-Qadir, Shaun G. Goodman, Michael E. Farkouh, Ruth Croxford, Patrick R. Lawler, John A. Spertus, Douglas S. Lee
Summary: The study found that early coronary angiography in patients with acute heart failure is associated with lower all-cause mortality, cardiovascular death, and heart failure readmissions, and increased rates of coronary revascularization.
EUROPEAN HEART JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Soon-Young Jung, Hye Hyeon Yun, Ji Hee Lim, Dong-Hyung Lee, Sung Bin Seo, Ji-Ye Baek, Jeehan Lee, Kyunghyun Yoo, Hyungmin Kim, Hong Lim Kim, Jeong-Hwa Lee
Summary: In this study, hepatocyte-specific knockout mice lacking the BIS gene were generated. These mice showed no abnormalities in metabolic function and survival, but exhibited defective autophagy, increased oxidative stress, and senescence in the liver. The results suggest a role for BIS in maintaining liver regeneration potential under pathological conditions.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Josiah E. E. Hardesty, Jeffrey B. B. Warner, Ying L. L. Song, Eric C. C. Rouchka, Craig J. J. McClain, Dennis R. R. Warner, Irina A. A. Kirpich
Summary: Alcohol-associated liver disease (ALD) is a major health issue with limited treatment options. Alcohol-associated hepatitis (AH) is a severe form of ALD and has a high mortality rate. This study investigated the therapeutic efficacy and mechanisms of Resolvin D1 (RvD1) in treating liver injury and inflammation in an animal model of severe AH.
Article
Peripheral Vascular Disease
Qiongxin Wang, Shirley Wang, Zhongjie Sun
Summary: The study found that deletion of the Klotho gene leads to hyperphosphatemia and aortic aneurysm formation, while intervention through molecular mechanisms and nutritional control can prevent the progression of aortic aneurysm.
Article
Medicine, Research & Experimental
Lei Gu, Xin He, Yanqiu Zhang, Shenglan Li, Jie Tang, Ruixue Ma, Xinyi Yang, Hao Huang, Yu Peng, Yanyun Xie, Zhangzhe Peng, Jie Meng, Gaoyun Hu, Lijian Tao, Xiaowei Liu
Summary: AKF-PD can protect against acute liver failure caused by acetaminophen or lipopolysaccharide/D-galactosamine, partially through regulating the MKK4/JNK pathway. It reduces necrosis, apoptosis, reactive oxygen species, and improves survival. It also shows protective effects in cell lines and primary hepatocytes. AKF-PD may be a novel candidate drug for acute liver failure.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Multidisciplinary Sciences
Jerry Wang, Ying Xia, Aizhu Lu, Hongwei Wang, Darryl R. Davis, Peter Liu, Rob S. Beanlands, Wenbin Liang
Summary: This study demonstrates that susceptibility to ventricular tachycardias in mice after myocardial infarction is reduced in the chronic phase with cardiomyocyte-specific beta-catenin deletion, primarily through attenuated structural remodeling rather than ion channel gene alterations.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Ina Luiken, Stephan Eisenmann, Jakob Garbe, Hanna Sternby, Robert C. Verdonk, Alexandra Dimova, Povilas Ignatavicius, Lucas Ilzarbe, Peeter Koiva, Anne K. Penttila, Sara Regner, Johannes Dober, Walter A. Wohlgemuth, Richard Brill, Patrick Michl, Jonas Rosendahl, Marko Damm
Summary: This study evaluated the prevalence and prognostic relevance of early pleuropulmonary pathologies and pre-existing chronic lung diseases in patients with acute pancreatitis. The results showed that bilateral pleural effusions and pre-existing chronic lung diseases were independent predictors of severe acute pancreatitis.
Article
Biochemistry & Molecular Biology
M. Herranz-Iturbide, J. Lopez-Luque, E. Gonzalez-Sanchez, D. Caballero-Diaz, E. Crosas-Molist, B. Martin-Mur, M. Gut, A. Esteve-Codina, V Jaquet, J. X. Jiang, N. J. Torok, I Fabregat
Summary: NOX4 plays a role in promoting hepatocyte proliferation and repair during liver regeneration, with its deletion leading to accelerated liver recovery. NOX4-deleted mice show faster cell proliferation and better liver weight restoration, suggesting the potential of NOX4 as a therapeutic target for improving liver regeneration.
Article
Gastroenterology & Hepatology
Alexandra Shingina, Nizar Mukhtar, Jamile Wakim-Fleming, Saleh Alqahtani, Robert J. Wong, Berkeley N. Limketkai, Anne M. Larson, Lafaine Grant
Summary: Acute liver failure (ALF) is a rare, potentially reversible condition that causes severe liver damage and rapid clinical deterioration in patients without preexisting liver disease. Due to limited studies, guidelines provided by the American College of Gastroenterology serve as the official practice recommendations for the identification, treatment, and management of ALF. The guidelines were developed through a process of reviewing scientific evidence and using expert opinions when robust evidence was lacking. Individualized care should be applied in specific clinical scenarios due to the varied clinical presentations of ALF.
AMERICAN JOURNAL OF GASTROENTEROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yingli Zhong, Jiyong Xie, Suzhen Wen, Wenwu Wu, Li Tan, Mingguang Lei, Huazhong Shi, Jian-kang Zhu
PLANT MOLECULAR BIOLOGY
(2020)
Article
Biology
Chang Zhang, Lei Shen, Wei Yuan, Yuguo Liu, Ruochen Guo, Yangjun Luo, Zheng Zhan, Zhiqin Xie, Guohao Wu, Wenwu Wu, Ying Feng
COMMUNICATIONS BIOLOGY
(2020)
Article
Cell & Tissue Engineering
Yuguo Liu, Yangjun Luo, Lei Shen, Ruochen Guo, Zheng Zhan, Ningyang Yuan, Rula Sha, Wenju Qian, Zhenzhen Wang, Zhiqin Xie, Wenwu Wu, Ying Feng
Article
Plant Sciences
Xiaona Cui, Kaili Gao, Linjuan Wang, Mengyang Lv, Ziwen Li, Donghua Zheng, Wenwu Wu, Wen Yao, Liying Ding, Xiao Li, Jian-Kang Zhu, Hairong Zhang
Summary: AtGCN1 plays a crucial role in gene expression and translation in Arabidopsis, affecting flower development, seed growth, and various physiological processes.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Xijuan Zhao, Lingling Tan, Shuo Wang, Yirong Shen, Liangyu Guo, Xiaoxue Ye, Shenkui Liu, Ying Feng, Wenwu Wu
Summary: This study identified 24 PtSR genes in Popolus trichocarpa, showing their constitutive expression in roots, stems, and leaves, and their response to various stresses. PtSCL30 may act as a negative regulator under cold and salt stress in woody plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Xiaoxue Ye, Xijuan Zhao, Yajun Sun, Meijiao Zhang, Shuang Feng, Aimin Zhou, Wenwu Wu, Shurong Ma, Shenkui Liu
Summary: This study investigates the morphological characteristics and potential molecular mechanisms of different types of buds in dioecious Salix. It reveals the development dynamics, gene expression differences, and dynamic changes in sex determination mechanisms of flower buds and leaf buds. The study provides new insights into the molecular mechanisms of dioecious buds and their development, dormancy breaking, flowering, and sexual association.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Hua Liu, Hongna Shang, Huan Yang, Wenjie Liu, Daisuke Tsugama, Ken-Ichi Nonomura, Aimin Zhou, Wenwu Wu, Tetsuo Takano, Shenkui Liu
Summary: The development of floral organs is regulated by a complex network of homeotic genes, and the signaling of gibberellin (GA) is essential for this process. In this study, researchers discovered that the protein MAC5A, which contains an RNA-binding motif, is involved in the development of sepals, petals, and stamens. MAC5A interacts with RBE, a repressor of AG expression, and is required for normal stamen development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Xiaoxue Ye, Shuo Wang, Xijuan Zhao, Ni Gao, Yao Wang, Yanmei Yang, Ernest Wu, Cheng Jiang, Yuxiang Cheng, Wenwu Wu, Shenkui Liu
Summary: Long non-coding RNAs (lncRNAs) play important roles in regulating gene expression under salt stress. This study identified 2988 high-confidence lncRNAs, including 1183 salt-induced differentially expressed lncRNAs, in Populus trichocarpa. It was found that lncRNAs can regulate neighboring genes in a cis-regulatory manner and contribute to distant gene expression in a trans-regulatory manner. Additionally, a specific lncRNA, Ptlinc-NAC72, was shown to regulate the expression and functional roles of homologous genes PtNAC72.A/B under long-term salt stress.
Article
Plant Sciences
Shuo Wang, Yirong Shen, Liangyu Guo, Lingling Tan, Xiaoxue Ye, Yanmei Yang, Xijuan Zhao, Yuqi Nie, Deyin Deng, Shenkui Liu, Wenwu Wu
Summary: This study identified 36 TCP genes in Populus trichocarpa and found that this significant innovation was mainly due to recent whole genome duplication (rWGD) after the divergence from Arabidopsis. The study also showed that the expressions of these genes varied among different plant parts and could be regulated by abiotic stresses. Furthermore, a cold-associated regulatory module was identified, suggesting the neofunctionalization of PtrTCP genes in response to environmental changes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Fuqiang Cui, Xiaoxiao Li, Wenwu Wu, Wenbo Luo, Ying Wu, Mikael Brosche, Kirk Overmyer
Summary: MYB108/BOS1 enhances cell death in the gain-of-function mutant bos1-1 after fungal infection and mechanical wounding. Programmed cell death (PCD) is integral to plant life and required for stress responses, immunity, and development. Our understanding of the regulation of PCD is incomplete, especially concerning regulators involved in multiple divergent processes. The botrytis-susceptible (bos1) mutant of Arabidopsis is highly susceptible to fungal infection by Botrytis cinerea (Botrytis). BOS1 (also known as MYB108) regulates cell death propagation during plant responses to wounding. The bos1-1 allele contains a T-DNA insertion in the 5 '-untranslated region upstream of the start codon. This insertion results in elevated expression of BOS1/MYB108. We used clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated nuclease 9 (Cas9) system (CRISPR/Cas9) to create new bos1 alleles with disrupted exons, and found that these lines lacked the typical bos1-1 wounding and Botrytis phenotypes. They did exhibit reduced fertility, as was previously observed in other bos1 alleles. Resequencing of the bos1-1 genome confirmed the presence of a mannopine synthase (MAS) promoter at the T-DNA left border. Expression of the BOS1 gene under control of the MAS promoter in wild-type plants conferred the characteristic phenotypes of bos1-1: Botrytis sensitivity and response to wounding. Multiple overexpression lines demonstrated that BOS1 was involved in regulation of cell death propagation in a dosage-dependent manner. Our data indicate that bos1-1 is a gain-of-function mutant and that BOS1 function in regulation of fertility and Botrytis response can both be understood as misregulated cell death.
Article
Biochemistry & Molecular Biology
Yuqi Nie, Liangyu Guo, Fuqiang Cui, Yirong Shen, Xiaoxue Ye, Deyin Deng, Shuo Wang, Jianhua Zhu, Wenwu Wu
Summary: The study reveals the origin and evolution of C-repeat binding factors/dehydration-responsive element binding protein 1s (CBFs/DREB1s) and their regulatory network. It shows that CBF/DREB1 is an innovation evolved from tandem duplication-derived DREB III gene and has expanded in ancient and modern plants through gene duplication. The duplication events mainly occurred during periods of significant global temperature decrease and the duplicated CBF/DREB1 genes contributed to the rewiring of the regulatory network for cold tolerance in plants.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Liangyu Guo, Shuo Wang, Yuqi Nie, Yirong Shen, Xiaoxue Ye, Wenwu Wu
Summary: Most eudicot plants did not undergo recent whole-genome duplication (R-WGD), but they were still able to adapt to environmental changes. In this study, researchers found that the AP2/ERF transcription factor family was retained after R-WGDs in some eudicot plants and showed exceptional response to cold stimulation. By comparing plants with and without R-WGD, the study revealed convergent expansions of the AP2/ERF III and IX subfamilies through independent duplications and tandem duplications (TDs) after R-WGDs. These expansions coincided with times of global cooling, particularly around and/or after the Cretaceous-Paleogene (K-Pg) boundary. The co-retention and expansion of AP2/ERF III C-repeat binding factor (CBF) duplicates contributed to rewiring of cold-specific regulatory networks in different eudicot lineages.
PLANT COMMUNICATIONS
(2022)
Article
Plant Sciences
Liangyu Guo, Zhiming Xu, Shuo Wang, Yuqi Nie, Xiaoxue Ye, Xuejiao Jin, Jianhua Zhu, Wenwu Wu
Summary: This study investigates the regulatory networks involved in cold responses in three different species of rosids. It demonstrates that some transcription factors and RNA-binding proteins are conserved in the cold response across rosids, and it reveals a hierarchical regulation of differentially expressed genes and alternative splicing genes. The study also identifies a common enrichment related to circadian rhythm in the cold response pathways in all three studied species.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Thermodynamics
Yang Li, Maolin Yu, Yinming Bai, Zhaoyang Hou, Huijie Zhang, Wenwu Wu
Summary: In this study, heat conductive paths were created on the bearing seat and spindle housing to enhance heat dissipation. The temperature distributions of models with and without heat conductive paths were investigated. It was found that the heat dissipation significantly improved with the use of heat conductive paths, and increasing the number and diameter of paths led to lower internal temperatures and reduced temperature difference.
ADVANCES IN MECHANICAL ENGINEERING
(2023)
Article
Multidisciplinary Sciences
Shuo Wang, Yixian Zhang, Xiaoxue Ye, Yirong Shen, Hua Liu, Xijuan Zhao, Liangyu Guo, Lu Cao, Yunfei Du, Wenwu Wu
Summary: Based on high-throughput sequencing, this study selected 11 representative angiosperm species and examined their transcriptome changes during cold adaptation. The findings provide valuable insights into the conserved and evolutionary mechanisms of angiosperms in response to cold stress.