期刊
MOLECULAR AND CELLULAR BIOCHEMISTRY
卷 414, 期 1-2, 页码 37-46出版社
SPRINGER
DOI: 10.1007/s11010-016-2656-7
关键词
Bovine; Skeletal muscle satellite cells; Microrna-128; Sp1; Myogenic differentiation
类别
资金
- National Natural Science Foundation of China [31201021]
- Natural Science Foundation of Tianjin [13JCQNJC14600]
- Open Subjects for the Major Basic Research Program of Science and Technology Department of Inner Mongolia Autonomous Region [20130902]
- Excellent Young Teachers Program of Tianjin [J010070514]
- Second-level Candidates Training Project of 131 Innovative Talents of Tianjin [J01005021104]
MicroRNAs (miRNAs) play essential roles in muscle cell proliferation and differentiation. The muscle-specific miRNAs miR-1 and miR-206 have been shown to regulate muscle development and promote myogenic differentiation; however, it is likely that a number of other miRNAs play important roles in regulating myogenesis as well. microRNA-128 (miR-128) has been reported to be highly expressed in brain and skeletal muscle, and we found that miR-128 is also up-regulated during bovine skeletal muscle satellite cell differentiation using microarray analysis and qRT-PCR. However, little is known about the functions of miR-128 in bovine skeletal muscle satellite cell development. In this study, we investigated the biological functions of miR-128 in bovine skeletal muscle cell development. Using a dual-luciferase reporter assay, we confirmed that miR-128 regulates the Sp1 gene. Over-expression of miR-128 reduced Sp1 protein levels and inhibited muscle satellite cell proliferation and differentiation. Inhibition of miR-128 increased Sp1 protein levels and promoted muscle satellite cell differentiation but also suppressed proliferation. Changes in miR-128 and Sp1 expression levels also affected the protein levels of MyoD and CDKN1A. Sp1, an activator of MyoD and a suppressor of CDKN1A, plays an important role in bovine muscle cell proliferation and differentiation. The results of our study reveal a mechanism by which miR-128 regulates bovine skeletal muscle satellite cell proliferation and myogenic differentiation via Sp1.
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