Optimizing conditions to construct artificial cells using commercial in vitro transcription-translation system (PUREfrex2.0)

A highly photostable and bright green fluorescent protein

(2022) Masahiko Hirano et al.   NATURE BIOTECHNOLOGY

Mode selection mechanism in traveling and standing waves revealed by Min wave reconstituted in artificial cells

(2022) Sakura Takada et al.   Science Advances

In vitro assembly, positioning and contraction of a division ring in minimal cells

(2022) Shunshi Kohyama et al.   Nature Communications

Phospholipid synthesis inside phospholipid membrane vesicles

(2022) Sumie Eto et al.   Communications Biology

Optimization and compartmentalization of a cell-free mixture of DNA amplification and protein translation

(2022) Fuhai Han et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Reconstitution of contractile actomyosin rings in vesicles

(2021) Thomas Litschel et al.   Nature Communications

Cell to Cell Signaling through Light in Artificial Cell Communities: Glowing Predator Lures Prey

(2021) Taniya Chakraborty et al.   ACS Nano

Identification of conditions for efficient cell-sized liposome preparation using commercially available reconstituted in vitro transcription-translation system

(2021) Atsuko Uyeda et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Shaping Liposomes by Cell-Free Expressed Bacterial Microtubules

(2021) Johannes Kattan et al.   ACS Synthetic Biology

Membrane molecular crowding enhances MreB polymerization to shape synthetic cells from spheres to rods

(2020) David Garenne et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Intercellular communication between artificial cells by allosteric amplification of a molecular signal

(2020) Bastiaan C. Buddingh’ et al.   Nature Communications

Improved designs for pET expression plasmids increase protein production yield in Escherichia coli

(2020) Patrick J. Shilling et al.   Communications Biology

Genetically controlled membrane synthesis in liposomes

(2020) Duco Blanken et al.   Nature Communications

Designing Artificial Cells towards a New Generation of Biosensors

(2020) Margrethe A. Boyd et al.   TRENDS IN BIOTECHNOLOGY

Artificial photosynthetic cell producing energy for protein synthesis

(2019) Samuel Berhanu et al.   Nature Communications

Gene Expression Inside Liposomes: From Early Studies to Current Protocols

(2019) Pasquale Stano   CHEMISTRY-A EUROPEAN JOURNAL

Cell-sized confinement controls generation and stability of a protein wave for spatiotemporal regulation in cells

(2019) Shunshi Kohyama et al.   eLife

ilastik: interactive machine learning for (bio)image analysis

(2019) Stuart Berg et al.   NATURE METHODS

De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns

(2019) Elisa Godino et al.   Nature Communications

Self-replication of DNA by its encoded proteins in liposome-based synthetic cells

(2018) Pauline van Nies et al.   Nature Communications

De Novo Synthesis of Basal Bacterial Cell Division Proteins FtsZ, FtsA, and ZipA Inside Giant Vesicles

(2018) Takumi Furusato et al.   ACS Synthetic Biology

Cell-Free Phospholipid Biosynthesis by Gene-Encoded Enzymes Reconstituted in Liposomes

(2016) Andrew Scott et al.   PLoS One

The PURE system for the cell-free synthesis of membrane proteins

(2015) Yutetsu Kuruma et al.   Nature Protocols

Mg2+, K+, and the Ribosome

(2014) K. H. Nierhaus   JOURNAL OF BACTERIOLOGY

Integrating reductive and synthetic approaches in biology using man-made cell-like compartments

(2014) Wataru Aoki et al.   Scientific Reports

Translation Enhancer Improves the Ribosome Liberation from Translation Initiation

(2013) Shuntaro Takahashi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Liposome division by a simple bacterial division machinery

(2013) M. Osawa et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cell-Free Protein Synthesis inside Giant Unilamellar Vesicles Analyzed by Flow Cytometry

(2012) Koji Nishimura et al.   LANGMUIR

NIH Image to ImageJ: 25 years of image analysis

(2012) Caroline A Schneider et al.   NATURE METHODS

flowCore: a Bioconductor package for high throughput flow cytometry

(2009) Florian Hahne et al.   BMC BIOINFORMATICS

Quantifying epistatic interactions among the components constituting the protein translation system

(2009) Tomoaki Matsuura et al.   Molecular Systems Biology

A synthetic biology approach to the construction of membrane proteins in semi-synthetic minimal cells

(2008) Yutetsu Kuruma et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Replication of Genetic Information with Self-Encoded Replicase in Liposomes

(2008) Hiroshi Kita et al.   CHEMBIOCHEM

Reconstitution of Contractile FtsZ Rings in Liposomes

(2008) M. Osawa et al.   SCIENCE