Article
Cell Biology
Yan Yan, Karl N. Krecke, Aditi S. Bapat, Tingyuan Yang, Michael W. Lopresti, Douglas G. Mashek, Ameeta Kelekar
Summary: The study revealed a novel role for PHLPP2 in targeting AMP-activated protein kinase (AMPK) to suppress the survival response of leukemia cells under glucose limitation. Silencing PHLPP2 prolonged the survival of leukemia cells by promoting AMPK-mediated fatty acid oxidation. This discovery could lead to better therapeutic strategies for both cancer and metabolic diseases.
CELL DEATH & DISEASE
(2021)
Article
Medicine, Research & Experimental
Wenjing Zhang, Yu Sun, Lu Bai, Lili Zhi, Yun Yang, Qingzhi Zhao, Chaoqun Chen, Yangfan Qi, Wenting Gao, Wenxia He, Luning Wang, Dan Chen, Shujun Fan, Huan Chen, Hai-Long Piao, Qinglong Qiao, Zhaochao Xu, Jinrui Zhang, Jinyao Zhao, Sirui Zhang, Yue Yin, Chao Peng, Xiaoling Li, Quentin Liu, Han Liu, Yang Wang
Summary: RBMS1 is a key regulator of ferroptosis in lung cancer development and its high expression is associated with reduced patient survival. Depletion or inhibition of RBMS1 can promote ferroptosis and sensitize radioresistant lung cancer cells to radiotherapy. RBMS1 interacts with eIF3d to regulate translation of SLC7A11, playing a critical role in ferroptosis evasion during lung cancer progression.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Oncology
Sameer Mirza, Achyuth Kalluchi, Mohsin Raza, Irfana Saleem, Bhopal Mohapatra, Dhananjaya Pal, Michel M. Ouellette, Fang Qiu, Lulu Yu, Alexei Lobanov, Zhi-Ming Zheng, Ying Zhang, Mansour A. Alsaleem, Emad A. Rakha, Hamid Band, M. Jordan Rowley, Vimla Band
Summary: This study reveals a novel role of ECD in viral and cellular mRNA splicing to support HPV-driven oncogenesis. ECD interacts with high-risk HPV E6 proteins and is overexpressed in cervical and head and neck squamous cell carcinoma. Its overexpression predicts shorter survival in patients and ECD knockdown impairs oncogenic behavior in cervical cancer cells.
MOLECULAR CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Adam De Jesus, Farnaz Keyhani-Nejad, Carolina M. Pusec, Lauren Goodman, Justin A. Geier, Joshua S. Stoolman, Paulina J. Stanczyk, Tivoli Nguyen, Kai Xu, Krishna V. Suresh, Yihan Chen, Arianne E. Rodriguez, Jason S. Shapiro, Hsiang-Chun Chang, Chunlei Chen, Kriti P. Shah, Issam Ben-Sahra, Brian T. Layden, Navdeep S. Chandel, Samuel E. Weinberg, Hossein Ardehali
Summary: The product of hexokinase enzymes, glucose-6-phosphate, can be metabolized through glycolysis or directed to alternative metabolic routes. The presence of N-terminal mitochondrial binding domain (MBD) in HK1 alters glucose metabolism through regulation of GAPDH.
Article
Cardiac & Cardiovascular Systems
Peiheng Gan, Zhaoning Wang, Svetlana Bezprozvannaya, John R. McAnally, Wei Tan, Hui Li, Rhonda Bassel-Duby, Ning Liu, Eric N. Olson
Summary: This study identifies the crucial role of RBPMS in regulating cardiomyocyte contraction and cardiac function through modulating alternative splicing of sarcomeric genes.
CARDIOVASCULAR RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Calla B. Shubin, Rini Mayangsari, Ariel D. Swett, Carol W. Greider
Summary: In budding yeast, Rif1 negatively regulates telomere length through a region with HEAT repeats that mediate telomere length regulation, separate from its proposed DNA binding function. Conserved amino acids from position 436 to 577 play a crucial role in telomere length regulation.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Ahmed M. Malik, Josephine J. Wu, Christie A. Gillies, Quinlan A. Doctrove, Xingli Li, Haoran Huang, Elizabeth H. M. Tank, Vikram G. Shakkottai, Sami Barmada
Summary: RNA-binding protein (RBP) dysfunction plays a fundamental role in amyotrophic lateral sclerosis (ALS) and related neuromuscular disorders. This study shows that activity-dependent processes regulate the levels and functions of the RBP Matrin 3 (MATR3). Glutamatergic activity drives MATR3 degradation, and mutations in MATR3 render it resistant to degradation, suggesting a link between activity-dependent MATR3 regulation and disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Luning Wang, Xiaohong Zhang, Junxiu Sheng, Lei Chen, Lili Zhi, Yangfan Qi, Qianqian Zheng, Linlin Wang, Jinrui Zhang, Jinyao Zhao, Yang Wang, Shu-Xin Liu, Ming-Zhong Sun, Wenjing Zhang
Summary: Cellular senescence is a powerful mechanism for inhibiting the proliferation of cancer cells, and RNA-binding proteins (RBPs) play important roles in regulating senescence. This study discovered that the RBP RBM4 contributes to cellular senescence and its depletion induces senescence in various cell types, including cancer cells. Furthermore, the depletion of RBM4 inhibits cancer cell progression and is mediated by the regulation of SERPINE1 through miR-1244.
CELL DEATH & DISEASE
(2023)
Article
Infectious Diseases
Mingxia Zheng, Keting Zhu, Huagang Peng, Weilong Shang, Yan Zhao, Shuguang Lu, Xiancai Rao, Ming Li, Renjie Zhou, Gang Li
Summary: This study elucidates a novel model of CcpA-controlled Staphylococcus aureus biofilm through direct inhibition of staphylokinase expression, highlighting the multifaceted roles and multiple networks regulated by CcpA.
Article
Multidisciplinary Sciences
Guanfeng Xu, Hao Lyu, Yangqin Yi, Yuling Peng, Qili Feng, Qisheng Song, Chengcheng Gong, Xuezhen Peng, Subba Reddy Palli, Sichun Zheng
Summary: This study reveals a regulatory mechanism involving intragenic DNA methylation that controls ovarian and embryonic developmental processes in the silkworm Bombyx mori, with DNA methylation enhancing gene expression near the transcription start site of ovarian genes. Additionally, methyl-DNA-binding domain proteins MBD2/3 bind to intragenic methyl-CpG fragments, recruiting the acetyltransferase Tip60 to promote histone H3K27 acetylation and gene expression.
Article
Multidisciplinary Sciences
Jiyoung Lee, Cristina Olivieri, Colin Ong, Larry R. Masterson, Suzana Gomes, Bok-Soon Lee, Florian Schaefer, Kristina Lorenz, Gianluigi Veglia, Marsha Rich Rosner
Summary: Phosphorylation of RKIP at S153 by PKC triggers a switch from inhibition of Raf to inhibition of GRK2, enhancing signaling by beta-AR that activates PKA. PKA-phosphorylated RKIP at S51 promotes beta-AR-activated PKA signaling by triggering feedback activation of PKA. This allosteric regulatory mechanism may have therapeutic potential for regulating PKA signaling in disease states.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Nicole Kaminski, Anne R. Wondisford, Youngho Kwon, Michelle Lee Lynskey, Ragini Bhargava, Jonathan Barroso-Gonzalez, Laura Garcia-Exposito, Boxue He, Meng Xu, Dattatreya Mellacheruvu, Simon C. Watkins, Mauro Modesti, Kyle M. Miller, Alexey Nesvizhskii, Huaiying Zhang, Patrick Sung, Roderick J. O'Sullivan
Summary: In this study, the researchers found that RAD51AP1 interacts with TERRA and utilizes it to generate D and R-loop HR intermediates. RAD51AP1 binds to and stabilizes TERRA-containing R-loops, playing a role in the suppression of TERRA and prevention of TRCs during ALT-HDR. These findings provide insights into the important role of RAD51AP1 in the ALT mechanism and regulation of TERRA.
Article
Cell Biology
Hironori Hojo, Taku Saito, Xinjun He, Qiuyu Guo, Shoko Onodera, Toshifumi Azuma, Michinori Koebis, Kazuki Nakao, Atsu Aiba, Masahide Seki, Yutaka Suzuki, Hiroyuki Okada, Sakae Tanaka, Ung-il Chung, Andrew P. McMahon, Shinsuke Ohba
Summary: This study investigated Runx2-DNA binding and chromatin accessibility in neonatal osteoblasts and chondrocytes, revealing that Runx2 interacts with cell-type-specific chromatin-accessible regions and enhances chromatin accessibility. It was found that Runx2 is essential for establishing chromatin accessibility in osteoblasts and specific enhancers driven by Runx2 contribute to normal osteoblast differentiation.
Article
Multidisciplinary Sciences
Marina P. Volegova, Cynthia Hermosillo, Jamie H. D. Cate
Summary: Improper regulation of translation initiation, especially overexpression of eIF3 subunits, is associated with increased translation of mRNAs involved in cell proliferation and cancer progression. The HLH motif of EIF3A controls translation of a small set of oncogenic mRNAs and modulates EIF4A1 function during translation initiation of select transcripts.
Article
Plant Sciences
Feng Xiong, Jing-Jing Ren, Yu-Yi Wang, Zhou Zhou, Hao-Dong Qi, Marisa S. Otegui, Xiu-Ling Wang
Summary: This study identified the retention and splicing (RES) complex in Arabidopsis and demonstrated its importance in cell proliferation, early embryogenesis, and root development. The RES complex interacts with various proteins involved in spliceosome assembly and recognition of splice sites, further emphasizing its role in pre-mRNA splicing.