Optimization of astaxanthin accumulation in a new Haematococcus lacustris (CZJ) strain from China via response surface methodology

To realize the applied potentials, response surface methodology was attempted to optimize astaxanthin accumulation in a newly isolated Haematococcus lacustris (CZJ) strain from China. Analysis of the astaxanthin metabolic pathway was also conducted. The results showed that 200 similar to 500 mu mol photons m(-2) s(-1) light intensity, 0.25 similar to 1 g L-1 sodium acetate and 3 similar to 12 mg L-1 salicylic acid can affect pigments or biomass to increase astaxanthin concentration in CZJ. These processes were regulated by corresponding enzyme activities and gene expressions. However, 1 similar to 4 g L-1 sodium chloride and 2.5 similar to 10 mg L-1 ferrous sulfate had restraining effects on astaxanthin accumulation in CZJ. Based on response surface methodology, the combined condition for optimal astaxanthin concentration was light intensity of 489 mu mol photons m(-2) s(-1)-sodium acetate concentration of 1 g L-1 salicylic acid concentration of 10 mg L-1. Under this optimized condition, the astaxanthin concentration increased to 6.50 mg L-1. These results supplemented the property of a newly isolated H. lacustris strain from China and partly improved its application potential. The superiority of response surface methodology was also verified. This study provides important references for optimizing astaxanthin accumulation in Haematococcus strains.

Automated summary

Response surface methodology was used to optimize astaxanthin accumulation in a newly isolated Haematococcus lacustris strain from China. The study identified the optimal conditions for light intensity, sodium acetate concentration, and salicylic acid concentration that resulted in increased astaxanthin concentration in the strain. The study also demonstrated the inhibitory effects of sodium chloride and ferrous sulfate on astaxanthin accumulation. The results provided important references for optimizing astaxanthin accumulation in Haematococcus strains.