Impact of Canine Amniotic Mesenchymal Stem Cell Conditioned Media on the Wound Healing Process: In Vitro and In Vivo Study

The aim of this study was to provide a beneficial treatment effect of mesenchymal stem cell products derived from the canine amniotic membrane (AM-MSC) on the complicated wound healing process in dogs. AM-MSCs were characterized in terms of morphology, phenotypic profile, and multilineage differentiation potential. The in vitro study of the effect of canine amniotic mesenchymal stem cell conditioned media (AMMSC-CM) on a primary skin fibroblast cell culture scratch assay showed a decrease in the measured scratch area of about 66.39% against the negative control (Dulbecco's Modified Eagle's Medium-32.55%) and the positive control (Dulbecco's Modified Eagle's Medium supplemented with FGF2, N2, B27, and EGF-82.077%) after 72 h treatment. In the experimental study, seven dogs with complicated nonhealing wounds were treated with a combination of antibiotics, NSAIDs, and local AMMSC-CM application. After 15 days of therapy, we observed a 98.47% reduction in the wound surface area as opposed to 57.135% in the control group treated by conventional therapy based on debridement of necrotic tissue, antibiotic therapy, pain management, and change of wound dressing.

Automated summary

The aim of this study was to investigate the beneficial treatment effect of canine amniotic membrane-derived mesenchymal stem cell products (AM-MSC) on complicated wound healing in dogs. Characterization of AM-MSCs showed their distinctive features and potential for differentiation. In vitro studies demonstrated a significant decrease in scratch area on skin fibroblast cultures treated with canine amniotic mesenchymal stem cell conditioned media (AMMSC-CM). In the experimental study, the combination therapy of antibiotics, NSAIDs, and local AMMSC-CM application resulted in a substantial reduction in wound surface area compared to conventional therapy.