期刊
MICROCHIMICA ACTA
卷 184, 期 1, 页码 203-210出版社
SPRINGER WIEN
DOI: 10.1007/s00604-016-2011-4
关键词
Veterinary antibiotic; Aptasensor; Molybdenum disulfide MoS2; Exfoliation; Fluorescence resonance energy transfer (FRET); Milk analysis
资金
- National Science & Technology Pillar Program of China [2014BAD13B05]
- Yangling Agricultural Hi-tech Industries Demonstration Zone [2014NY-35]
A rapid and sensitive aptamer-based assay is described for kanamycin, a veterinary antibiotic with neurotoxic side effects. It is based on a novel FRET pair consisting of fluorescent carbon dots and layered MoS2. This donor-acceptor pair (operated at excitation/emission wavelengths of 380/440 nm) shows fluorescence recovery efficiencies reaching 93 %. By taking advantages of aptamer-induced fluorescence quenching and recovery, kanamycin can be quantified in the of 4-25 mu M concentration range, with a detection limit of 1.1 mu M. The method displays good specificity and was applied to the determination of kanamycin in spiked milk where it gave recoveries ranging from 85 % to 102 %, demonstrating that the method serves as a promising tool for the rapid detection of kanamycin in milk and other animal-derived foodstuff.
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