期刊
MICROCHIMICA ACTA
卷 183, 期 12, 页码 3119-3126出版社
SPRINGER WIEN
DOI: 10.1007/s00604-016-1954-9
关键词
Nanostructures; Biosensor; Silicon substrate; FTIR; AFM; Chemiluminescent detection
资金
- Ministry of Higher Education (MOHE) [9003-00481]
The authors introduce a method for spatially arranged DNA immobilization on 10-nm gold nanoparticles (GNP) deposited on a silicon substrate carrying nanogapped interdigitated electrodes. The GNPs are covalently bound to the surface via silane chemistry, and the single steps of fabrication are monitored by FTIR spectroscopy and atomic force microscopy. This GNP deposition technique is shown to reduce the size of the nanogaps to 130 nm. FTIR also was used to monitor the immobilization of DNA on the surface of the interdigitated electrodes. This method allows DNA to be immobilized in a uniform and homogenous way. The utility of the method is demonstrated by immobilizing probe DNA on the surface and detecting target DNA specific for the human papilloma virus via fluorescence with a detection limit as low as 1 pM. In our perception, this method for GNP-mediated DNA immobilization enables high-performance sensing of a wide range of target (analyte) DNA.
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