期刊
MICROCHIMICA ACTA
卷 183, 期 5, 页码 1563-1570出版社
SPRINGER WIEN
DOI: 10.1007/s00604-016-1774-y
关键词
FRET; Ag@SiO2; Transmission electron microscopy; Graphene oxide; Bioconjugation; Help DNA
资金
- National Natural Science Foundation of China [21301103, 21501106]
- 47th Scientific Research Foundation for the returned overseas Chinese scholars
- Shandong Natural Science Foundation [ZR2012FZ007]
- Shandong Province High Education Research And Development program [J13LA08]
A sensitive on-off fluorescent protocol for thrombin detection is demonstrated. Firstly, thrombin aptamers which hybridize with labeled help DNA were immobilized on the surface of Ag@SiO2 nanoparticles (NPs). The silver core causes the label Cy5 to display strong metal-enhanced fluorescence. On addition of thrombin and graphene oxide (GO), thrombin (with its high affinity for the aptamers) displaces the Cy5-labeled help DNA which then binds to the surface of GO via pi-stacking, causing fluorescence quenching of Cy5. The findings were used to design a thrombin assay that has a 0.05 nM detection limit and excellent selectivity. It was applied to the quantification of thrombin in spiked serum samples where is showed recoveries ranging from 97 % to 107 %, with relative standard deviations between 2.2 and 4.5 %.
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