期刊
MICROCHIMICA ACTA
卷 184, 期 2, 页码 633-639出版社
SPRINGER WIEN
DOI: 10.1007/s00604-016-2042-x
关键词
Biosensor; Allergen detection; Graphene oxide; Tetramethylbenzidine; Horse radish peroxidase; Binding assay
资金
- National High-tech R&D Program (863 Program) [2013AA102202]
- Special Project on the Integration of Industry, Education and Research of Guangdong Province [2013B091100012]
Shrimp allergy is a common cause of allergenic diseases. The authors describe an aptamer-based assay for tropomyosin, one of the major allergens of shrimp. After six rounds of SELEX based on nitrocellulose membrane, several aptamers with high affinity (K-d = 109.6 nM) were identified that can selectively recognize tropomyosin but not other proteins over a large range of concentrations. By using a fluorescent and graphene oxide based platform, a tropomyosin assay was worked out that has a 4.2 nM detection limit and work in the 0.5 to 50 mu g center dot mL(-1) concentration range. The aptamers selected hold a great potential for tropomyosin detection in practical biosensor assays.
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