期刊
MICROBIOLOGY-SGM
卷 162, 期 -, 页码 954-965出版社
MICROBIOLOGY SOC
DOI: 10.1099/mic.0.000289
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资金
- APC Microbiome Institute, a research centre - Science Foundation Ireland [SFI/12/RC/2273]
- Higher Education Authority of Ireland
- European Regional Development Fund
- Healthcare Innovation Program me Award from Science Foundation Ireland
- Johnson and Johnson Services Inc. (JJSI) [08/IN1/B1843 HIPA10]
- Science Foundation Ireland (SFI) [08/IN1/B1843 HIPA10] Funding Source: Science Foundation Ireland (SFI)
Adherent-invasive Escherichia coli (AIEC) have been implicated in the aetiology of Crohn's disease (CD), a chronic inflammatory bowel condition. It has been proposed that AIEC-infected macrophages produce high levels of pro-inflammatory cytokines thus contributing to the inflammation observed in CD. AIEC can replicate in macrophages and we wanted to determine if bacterial replication was linked to the high level of cytokine production associated with AIEC-infected macrophages. Therefore, we undertook a genetic analysis of the metabolic requirements for AIEC replication in the macrophage and we show that AIEC replication in this niche is dependent on bacterial glycolysis. In addition, our analyses indicate that AIEC have access to a wide range of nutrients in the macrophage, although the levels of purines and pyrimidines do appear to be limiting. Finally, we show that the macrophage response to AIEC infection is indistinguishable from the response to the non-replicating glycolysis mutant (Delta pfkAB) and a nonpathogenic strain of E. coli, MG1655. Therefore, AIEC does not appear to subvert the normal macrophage response to E. coli during infection.
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