Article
Microbiology
Shayla Hesse, Natalia Malachowa, Adeline R. Porter, Brett Freedman, Scott D. Kobayashi, Donald J. Gardner, Dana P. Scott, Sankar Adhya, Frank R. DeLeo
Summary: This study demonstrates that prompt treatment with two bacteriophages can effectively treat Klebsiella pneumoniae ST258 bacteremia, with combination phage therapy showing the greatest increase in survival and the lowest frequency of phage resistance among bacteria. These findings support the utility of phage therapy as an approach for refractory ST258 infections and highlight the potential for enhancing treatment through strategic phage selection.
Article
Microbiology
Jungyu Seo, Yu Mi Wi, Jong Min Kim, Yae-Jean Kim, Kwan Soo Ko
Summary: This study revealed the common occurrence of colistin heteroresistance in KPC-2-producing Klebsiella pneumoniae isolates, which could lead to treatment failure. The presence of colistin-resistant subpopulations increased with exposure to colistin, indicating a potential evolution of resistance. Developing more reliable diagnostic methods is necessary to detect colistin heteroresistance and prevent treatment failures.
JOURNAL OF MICROBIOLOGY
(2021)
Article
Microbiology
Leticia B. Migliorini, Romario O. de Sales, Paula C. M. Koga, Andre M. Doi, Anja Poehlein, Alexandra R. Toniolo, Fernando G. Menezes, Marines D. Martino, Ana C. Gales, Holger Brueggemann, Patricia Severino
Summary: This study reports the genomic context of KPC coding genes bla(KPC-2), bla(KPC-3) and bla(KPC-30) in multidrug-resistant Klebsiella pneumoniae isolates from Brazil, with a focus on the prevalence and dissemination of bla(KPC)-harboring plasmids. It demonstrates the risk of horizontal spread of KPC coding genes and the high synteny of plasmid backbones among isolates, including the recent spread of bla(KPC-3) and bla(KPC-30).
Article
Immunology
Xiaoyu Liu, Yarong Wu, Ying Zhu, Peiyao Jia, Xue Li, Xinmiao Jia, Wei Yu, Yujun Cui, Ruifu Yang, Wei Xia, Yingchun Xu, Qiwen Yang
Summary: This study retrospectively screened 14 colistin-resistant Klebsiella pneumoniae strains, of which 6 were multidrug-resistant strains. The study revealed that the up-regulated expression of certain genes was associated with colistin resistance, and mgrB mutation was found to be the most common molecular mechanism of colistin resistance. In addition, the presence of hypervirulence genes and biofilm-producing genes was also identified. This suggests that colistin-resistant and hypervirulent multidrug-resistant Klebsiella pneumoniae could pose a severe challenge to public health.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Infectious Diseases
Sotiria Fergadaki, Georgios Renieris, Nikolaos Machairas, Labros Sabracos, Dionyssia-Irene Droggiti, Evangelos Misiakos, Evangelos J. Giamarellos-Bourboulis
Summary: Experimental studies demonstrated that tigecycline alone or in combination effectively prolongs survival and reduces bacterial load in KPC-Kp infections in mice.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Infectious Diseases
Efthymia Protonotariou, Georgios Meletis, Dimitrios Pilalas, Paraskevi Mantzana, Areti Tychala, Charalampos Kotzamanidis, Dimitra Papadopoulou, Theofilos Papadopoulos, Michalis Polemis, Simeon Metallidis, Lemonia Skoura
Summary: This study investigated the molecular epidemiology of carbapenemase-producing Klebsiella pneumoniae (CPKP) in the intensive care units of a Greek hospital and found extensive dissemination and sustained transmission of multiple strains, posing a threat to the selection of pathogens resistant to last-resort antimicrobial agents.
Article
Infectious Diseases
Qiaoyan Duan, Qi Wang, Shijun Sun, Qiaozhen Cui, Qi Ding, Ruobing Wang, Hui Wang
Summary: The nosocomial spread of carbapenem-resistant Enterobacterales (CRE) is common and places severe burdens on healthcare systems. The high-risk Klebsiella pneumoniae ST11 strain has a wide endemic area in China. This study monitored CRE genotypes and phenotypes in a tertiary hospital in North China from 2012 to 2020. The results showed that the ST11 strain carrying bla(NDM) exhibited strong adaptability and could potentially replace other strains to become an epidemic strain. Infection control and preventive measures should focus on the high-risk ST11-K. pneumoniae strain.
Article
Biochemistry & Molecular Biology
Agata Pruss, Pawel Kwiatkowski, Lukasz Lopusiewicz, Helena Masiuk, Peter Sobolewski, Karol Fijalkowski, Monika Sienkiewicz, Adam Smolak, Stefania Giedrys-Kalemba, Barbara Dolegowska
Summary: This study evaluated the electrical potential and chemical alterations in laboratory-induced colistin-resistant Klebsiella pneumoniae compared to susceptible strains, revealing differences in cell wall structures and LPS composition between the two strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Pushpa Kumari Nirwan, Nirupama Chatterjee, Rajesh Panwar, Mridu Dudeja, Namita Jaggi
Summary: This study investigated the molecular basis of colistin resistance in Klebsiella pneumoniae and its clonal transmission in a North Indian tertiary care hospital. Results indicated that colistin resistance was mainly attributed to mutations in PhoPQ and PmrAB TCS genes, with different sequence types sharing a common ancestor and showing clonal transmission patterns. These findings will contribute to the formulation of effective strategies for infection prevention and antimicrobial development.
JOURNAL OF ANTIBIOTICS
(2021)
Article
Microbiology
Veronica Franca Diniz Rocha, Matheus Sales Barbosa, Helena Ferreira Leal, Giulyana Evelyn Oliveira Silva, Nabila Monalisa Mendes Dantas Sales, Adriano de Souza Santos Monteiro, Jailton Azevedo, Allan Roberto Xavier Malheiros, Ledilce Almeida Ataide, Beatriz Meurer Moreira, Mitermayer Galvao Reis, Fabianna Marcia Maranhao Bahia, Joice Neves Reis
Summary: This study investigated the genetic relationship, antimicrobial susceptibility profile, and resistance mechanisms to ColR-CRKP isolates from patients infected/colonized in a tertiary hospital in Salvador, Bahia/Brazil. The study found a prolonged outbreak of ColR-CRKP in the region, suggesting possible cross-transmission.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Jiaxin Yu, Yu-Tzu Lin, Wei-Cheng Chen, Kun-Hao Tseng, Hsiu-Hsien Lin, Ni Tien, Chia-Fong Cho, Jhao-Yu Huang, Shinn-Jye Liang, Lu-Ching Ho, Yow-Wen Hsieh, Kai-Cheng Hsu, Mao-Wang Ho, Po-Ren Hsueh, Der-Yang Cho
Summary: The study aimed to develop a rapid prediction method for carbapenem-resistant Kleb-siella pneumoniae (CRKP) and colistin-resistant K. pneumoniae (ColRKP) based on routine MALDI-TOF mass spectrometry (MS) results to guide suitable and swift treatment strategies. By utilizing machine learning (ML), the accuracy and area under the curve of the ML model for distinguishing CRKP and CSKP were 0.8869 and 0.9551, respectively. Additionally, the ML model achieved an accuracy of 0.8361 and area under the curve of 0.8447 for differentiating ColRKP and ColIKP. The proposed model, combined with preliminary reporting of results, could potentially lead to improved patient survival through timely antibiotic intervention.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Chemistry, Multidisciplinary
Yishuai Lin, Ying Zhang, Shixing Liu, Dandan Ye, Liqiong Chen, Na Huang, Weiliang Zeng, Wenli Liao, Yizhou Zhan, Tieli Zhou, Jianming Cao
Summary: Colistin, considered as a last resort treatment for Gram-negative bacteria infections, is becoming ineffective against colistin-resistant strains of Escherichia coli and Klebsiella Pneumoniae. The combination of quercetin with colistin efficiently eradicates colistin-resistant bacteria by downregulating resistance genes and enhancing bacterial cell membrane damage. This colistin/quercetin combination shows promise as an alternative pathway against colistin-resistant infections.
FRONTIERS IN CHEMISTRY
(2021)
Article
Infectious Diseases
Irene Sanchez-Leon, Teresa Garcia-Martinez, Seydina M. Diene, Elena Perez-Nadales, Luis Martinez-Martinez, Jean-Marc Rolain
Summary: This study aimed to investigate heteroresistance in nine clinical isolates of Klebsiella pneumoniae producing OXA-48 and describe genomic changes in vitro. It was found that the heteroresistance in K. pneumoniae isolates was mainly attributed to insertions in the mgrB gene and point mutations in colistin resistance proteins. These findings contribute to the understanding of colistin resistance mechanisms in OXA-48-producing K. pneumoniae mutants.
Article
Agriculture, Multidisciplinary
Qiushuang Sheng, Xiaoning Hou, Yang Wang, Nan Wang, Xuming Deng, Zhongmei Wen, Dan Li, Li Li, Yonglin Zhou, Jianfeng Wang
Summary: Naringenin enhances the activity of colistin against colistin-resistant Klebsiella pneumoniae by inhibiting colistin resistance gene activity, repressing two-component system regulation, and accelerating oxidative damage mediated by reactive oxygen species. A lung-targeted delivery system of naringenin microspheres is designed to increase bioavailability and effectively potentiate colistin for Klebsiella pneumoniae infection.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Microbiology
Min Yi Lau, Fui Enn Teng, Kek Heng Chua, Sasheela Ponnampalavanar, Chun Wie Chong, Kartini Abdul Jabar, Cindy Shuan Ju Teh
Summary: The study reported the epidemiology of carbapenem-resistant Klebsiella pneumoniae (CRKP) in a Malaysian teaching hospital, with bla(OXA-48) identified as the predominant carbapenemase gene. Infection or colonization by CRKP, especially NDM-producers, central venous catheter usage, and stoma were associated with higher in-hospital mortality. Early detection of CRKP isolates was crucial due to the potential high mortality rate associated with infection.
Article
Infectious Diseases
Angeliki Mavroidi, Apostolos Liakopoulos, Stiliani Sarrou, Vivi Miriagou, Efthymia Petinaki
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2015)
Article
Microbiology
Angeliki Mavroidi, Sofia Likousi, Eleftheria Palla, Maria Katsiari, Zoi Roussou, Asimina Maguina, Evangelia D. Platsouka
JOURNAL OF MEDICAL MICROBIOLOGY
(2015)
Article
Infectious Diseases
Angeliki Mavroidi, Maria Katsiari, Eleftheria Palla, Sofia Likousi, Zoi Roussou, Charikleia Nikolaou, Evangelia D. Platsouka
MICROBIAL DRUG RESISTANCE
(2017)
Article
Infectious Diseases
M. Katsiari, A. Mavroidi, E. D. Platsouka, C. Nikolaou
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2018)
Article
Infectious Diseases
Apostolos Liakopoulos, Angeliki Mavroidi, Efstathios A. Katsifas, Alexandros Theodosiou, Amalia D. Karagouni, Vivi Miriagou, Efthymia Petinaki
BMC INFECTIOUS DISEASES
(2013)
Article
Pediatrics
Angeliki Mavroidi, Apostolos Liakopoulos, Antonios Gounaris, Maria Goudesidou, Katerina Gaitana, Vivi Miriagou, Efthymia Petinaki
Article
Infectious Diseases
Apostolos Liakopoulos, Angeliki Mavroidi, Sofia Vourli, Maria Panopoulou, Levantia Zachariadou, Stylianos Chatzipanagiotou, Iris Spiliopoulou, Loukia Zerva, Efthymia Petinaki
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2014)
Article
Infectious Diseases
Angeliki Mavroidi, Vivi Miriagou, Ergina Malli, Angelos Stefos, George N. Dalekos, Leonidas S. Tzouvelekis, Efthymia Petinaki
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2012)
Article
Infectious Diseases
Angeliki Mavroidi, Maria Katsiari, Sofia Likousi, Eleftheria Palla, Zoi Roussou, Charikleia Nikolaou, Christos Mathas, Eleni Merkouri, Evangelia D. Platsouka
MICROBIAL DRUG RESISTANCE
(2020)
Article
Infectious Diseases
Zoi Florou, Aggeliki Mavroidi, George Vatidis, Zoi Daniil, Konstantinos Gourgoulianis, Efi Petinaki
Summary: This study aimed to investigate the resistance rates and mutations of genes associated with first-line antituberculous drugs in M. tuberculosis/canettii isolates from Central Greece between 2010 and 2019. Despite phenotypic resistance, mutations were found in a significant number of isolates, including both common and novel mutations, highlighting the importance of molecular-based diagnostic tests for better detection of drug-resistant strains. The regional variations in mutation frequencies provide valuable insights for the development of improved diagnostic methods.
MICROBIAL DRUG RESISTANCE
(2021)
Article
Infectious Diseases
Maria Katsiari, Angeliki Mavroidi, Eleftheria Palla, Konstantina Zourla, Theodoros Alonistiotis, Kyriakos Ntorlis, Charikleia Nikolaou, Georgia Vrioni, Athanasios Tsakris
Summary: A rare case of COVID-19-associated pulmonary aspergillosis (CAPA) caused by A. niger in a Greek patient is reported. Diagnosis was based on specific criteria and a new algorithm for invasive pulmonary aspergillosis. Fungal identification, antigen testing, and PCR results were used to confirm the diagnosis.
Article
Microbiology
Maria Katsiari, Angeliki Mavroidi, Nikolaos Kesesidis, Eleftheria Palla, Konstantina Zourla, Kyriakos Ntorlis, Konstantinos Konstantinidis, Maria Laskou, Konstantinos Strigklis, Anastasios Sakkalis, Charikleia Nikolaou, Evangelia D. Platsouka, Ioannis Karakasiliotis, Georgia Vrioni, Athanasios Tsakris
Summary: The first five ICU cases of C. auris in Greece were reported. All C. auris isolates showed resistance to fluconazole, and three of them exhibited resistance to amphotericin B. Environmental screening also indicated the dissemination of C. auris in the ICU. The study confirms the emergence of C. auris as a yeast pathogen in the region during the COVID-19 pandemic.
Article
Infectious Diseases
Konstantina Gartzonika, Lida Politi, Angeliki Mavroidi, Andreas G. Tsantes, Nicholas Spanakis, Efthalia Priavali, Georgia Vrioni, Athanasios Tsakris
Summary: This study describes the epidemiological and molecular characteristics of a large-scale outbreak of NDM-1-producing Enterobacter cloacae complex in Greece, which is the largest clonal outbreak of its kind in Europe. The presence of NDM-1 gene was confirmed in the isolates and it was found to be associated with other resistance genes through genetic sequencing.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Infectious Diseases
Angeliki Mavroidi, Konstantina Gartzonika, Nick Spanakis, Elisavet Froukala, Christos Kittas, Georgia Vrioni, Athanasios Tsakris
Summary: In this study, comparative genomics of two representative outbreak isolates of multidrug-resistant Enterobacter cloacae complex were performed. It was found that these two isolates harbored multiple antibiotic resistance genes and various virulence factors, with differences in the genetic makeup of the bacterial cell surface and genomic islands. Additionally, diverse modes of acquisition and ongoing evolution of plasmids carrying beta-lactamase genes between the two strains were observed.