Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system

Gene Repression in Haloarchaea Using the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas I-B System

(2016) Aris-Edda Stachler et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural basis for promiscuous PAM recognition in type I–E Cascade from E. coli

(2016) Robert P. Hayes et al.   NATURE

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

Tunable switch mediated shikimate biosynthesis in an engineered non-auxotrophic Escherichia coli

(2016) Pengfei Gu et al.   Scientific Reports

Self-induced metabolic state switching by a tunable cell density sensor for microbial isopropanol production

(2015) Yuki Soma et al.   METABOLIC ENGINEERING

Application of CRISPRi for prokaryotic metabolic engineering involving multiple genes, a case study: Controllable P(3HB- co -4HB) biosynthesis

(2015) Li Lv et al.   METABOLIC ENGINEERING

CRISPR-Cas immunity in prokaryotes

(2015) Luciano A. Marraffini   NATURE

An updated evolutionary classification of CRISPR–Cas systems

(2015) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Comparison of individual component deletions in a glucose-specific phosphotransferase system revealed their different applications

(2015) Quanfeng Liang et al.   Scientific Reports

Directional R-Loop Formation by the CRISPR-Cas Surveillance Complex Cascade Provides Efficient Off-Target Site Rejection

(2015) Marius Rutkauskas et al.   Cell Reports

CRISPR MultiTargeter: A Web Tool to Find Common and Unique CRISPR Single Guide RNA Targets in a Set of Similar Sequences

(2015) Sergey V. Prykhozhij et al.   PLoS One

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

(2014) S. Bae et al.   BIOINFORMATICS

From a co-production design to an integrated single-cell biorefinery

(2014) Quanfeng Liang et al.   BIOTECHNOLOGY ADVANCES

Metabolic flux redirection from a central metabolic pathway toward a synthetic pathway using a metabolic toggle switch

(2014) Yuki Soma et al.   METABOLIC ENGINEERING

Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli

(2014) Hongtu Zhao et al.   NATURE

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

(2014) Samuel H. Sternberg et al.   NATURE

Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation

(2014) John G Doench et al.   NATURE BIOTECHNOLOGY

Efficient programmable gene silencing by Cascade

(2014) Devashish Rath et al.   NUCLEIC ACIDS RESEARCH

Repurposing endogenous type I CRISPR-Cas systems for programmable gene repression

(2014) Michelle L. Luo et al.   NUCLEIC ACIDS RESEARCH

CRISPR-Mediated Adaptive Immune Systems in Bacteria and Archaea

(2013) Rotem Sorek et al.   Annual Review of Biochemistry

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Efficient genome editing in plants using a CRISPR/Cas system

(2013) Zhengyan Feng et al.   CELL RESEARCH

In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus

(2013) Tomas Sinkunas et al.   EMBO JOURNAL

Constitutive expression of RyhB regulates the heme biosynthesis pathway and increases the 5-aminolevulinic acid accumulation inEscherichia coli

(2013) Fangfang Li et al.   FEMS MICROBIOLOGY LETTERS

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

(2013) Prashant Mali et al.   NATURE BIOTECHNOLOGY

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

CRISPR interference (CRISPRi) for sequence-specific control of gene expression

(2013) Matthew H Larson et al.   Nature Protocols

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system

(2013) David Bikard et al.   NUCLEIC ACIDS RESEARCH

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

A strategy of gene overexpression based on tandem repetitive promoters in Escherichia coli

(2012) Mingji Li et al.   Microbial Cell Factories

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Helicase dissociation and annealing of RNA-DNA hybrids byEscherichia coliCas3 protein

(2011) Jamieson A. L. Howard et al.   BIOCHEMICAL JOURNAL

Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription

(2011) Feng Zhang et al.   NATURE BIOTECHNOLOGY

Structural basis for CRISPR RNA-guided DNA recognition by Cascade

(2011) Matthijs M Jore et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions

(2011) B. Wiedenheft et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CRISPR/Cas, the Immune System of Bacteria and Archaea

(2010) P. Horvath et al.   SCIENCE

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

(2009) S. A. Bustin et al.   CLINICAL CHEMISTRY

Complete PHB mobilization in Escherichia coli enhances the stress tolerance: a potential biotechnological application

(2009) Qian Wang et al.   Microbial Cell Factories

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE