期刊
METHODS
卷 94, 期 -, 页码 19-26出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2015.09.025
关键词
Mechanotransduction; Tension; VE-cadherin; Endothelial cell
资金
- NIH [T32-CA009156, HL114388, GM029860, GM103723]
- American Cancer Society [PF-13-318-01]
- Agence National de la Recherche [ANR-13-JSV1-0008]
- European Union [304162]
- Institut National de la Sante et de la Recherche Medicale (INSERM)
- Agence Nationale de la Recherche (ANR) [ANR-13-JSV1-0008] Funding Source: Agence Nationale de la Recherche (ANR)
Cellular tension has implications in normal biology and pathology. Membrane adhesion receptors serve as conduits for mechanotransduction that lead to cellular responses. Ligand-conjugated magnetic beads are a useful tool in the study of how cells sense and respond to tension. Here we detail methods for their use in applying tension to cells and strategies for analyzing the results. We demonstrate the methods by analyzing mechanotransduction through VE-cadherin on endothelial cells using both permanent magnets and magnetic tweezers. (C) 2015 Elsevier Inc. All rights reserved.
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