Article
Microbiology
Parsa Ghadermazi, Angela Re, Luca Ricci, Siu Hung Joshua Chan
Summary: Gas fermentation is a promising method to convert low-cost waste gases into valuable chemicals. This study used modeling and kinetic ensemble modeling to analyze metabolic engineering interventions for increased 2,3-butanediol production and reduced carbon loss. The results suggested that interventions targeting by-products branching from acetyl-CoA and pyruvate are effective strategies.
Article
Biotechnology & Applied Microbiology
Mengyun Kou, Zhenzhen Cui, Jing Fu, Wei Dai, Zhiwen Wang, Tao Chen
Summary: In this study, a C. glutamicum strain was engineered to efficiently produce optically pure (2R,3R)-2,3-butanediol. Through genetic modification and fermentation engineering, the titer of (2R,3R)-2,3-butanediol reached a record-high level, making C. glutamicum a competitive candidate for commercial production of this compound.
MICROBIAL CELL FACTORIES
(2022)
Review
Biotechnology & Applied Microbiology
Jae Won Lee, Ye-Gi Lee, Yong-Su Jin, Christopher V. Rao
Summary: 2,3-Butanediol (2,3-BDO) is a promising commodity chemical with various industrial applications. Fermentation-based production of 2,3-BDO is considered a more sustainable alternative to petroleum-based chemical processes. Metabolic engineering strategies have been developed to improve the production of 2,3-BDO from inexpensive sugars by non-pathogenic microorganisms.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Chan Woo Song, Chelladurai Rathnasingh, Hyohak Song
Summary: This study demonstrates the development of non-mucoid and byproduct-free strains of Bacillus licheniformis for efficient synthesis of 2,3-butanediol. By deleting specific genes and optimizing culture conditions, the goal of high yield and minimal byproducts was achieved successfully.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biochemical Research Methods
Yu Liu, Xuecong Cen, Dehua Liu, Zhen Chen
Summary: Efforts to improve the production efficiency of (R)-1,3-BDO by Escherichia coli through different metabolic engineering strategies have successfully optimized key pathway enzymes, increased NADPH supply, optimized fermentation conditions, and reduced byproducts formation. The best engineered strain can efficiently produce (R)-1,3-BDO with a yield of 0.6 mol/mol glucose, corresponding to 60% of the theoretical yield. Additionally, the feasibility of aerobically producing 1,3-BDO via a new pathway using 3-hydroxybutyrate as an intermediate has been demonstrated.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Daniel Tinoco, Lucy Seldin, Paulo Luiz de Andrade Coutinho, Denise Maria Guimaraes Freire
Summary: Optimization of culture conditions is an effective strategy to improve bio-based 2,3-butanediol (2,3-BDO) production. In this study, a new and safe strain Paenibacillus peoriae NRRL BD-62 was used to achieve a 2,3-BDO yield of 90% in batch cultures at specific conditions. In fed-batch fermentation, a yield of 39.4 g/L of 2,3-BDO was obtained by adjusting the operational limitations and utilizing acetoin, lactic acid, and ethanol as substrates.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2023)
Review
Biotechnology & Applied Microbiology
Sofia Maina, Ashish A. Prabhu, Narisetty Vivek, Anestis Vlysidis, Apostolis Koutinas, Vinod Kumar
Summary: This review summarizes the applications of bio-based platform chemicals 2,3-butanediol (BDO) and acetoin in various industries, as well as the novel technological developments in genetic and metabolic engineering strategies for their production. The review also discusses the valorization of renewable feedstocks and the development of bioprocesses for the production of BDO and acetoin. Techno-economic aspects evaluating the viability and industrial potential of bio-based BDO production are presented.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Biotechnology & Applied Microbiology
Wensi Meng, Yongjia Zhang, Liting Ma, Chuanjuan Lu, Ping Xu, Cuiqing Ma, Chao Gao
Summary: The feasibility of non-sterilized fermentation using Vibrio natriegens as a microbial chassis and producing chemicals through metabolic engineering has been confirmed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Guangxin Huo, Maria R. Foulquie-Moreno, Johan M. Thevelein
Summary: The engineered microbial cell factories showed high production of 2,3-butanediol with the highest reported titer and close to the maximum theoretical yield, making them suitable for industrial implementation.
MICROBIAL CELL FACTORIES
(2022)
Article
Agricultural Engineering
Tayyab Islam, Thuan Phu Nguyen-Vo, Vivek Kumar Gaur, Junhak Lee, Sunghoon Park
Summary: The production of 1,3-butanediol from glucose was achieved by engineering a pathway in Escherichia coli and optimizing gene expression levels and culture conditions. The highest ever reported titer of 1,3-butanediol in biological production methods was achieved at 257 mM in 36 hours with a yield of 0.51 mol/mol. This study provides a promising strategy for sustainable synthesis of other acetyl-CoA-derived chemicals using microbial cell factories.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Tayyab Islam, Thuan Phu Nguyen-Vo, Seunghyun Cho, Junhak Lee, Vivek Kumar Gaur, Sunghoon Park
Summary: This study focuses on metabolic engineering of Escherichia coli to efficiently produce 1,3-BDO. By reducing the accumulation of intermediate metabolites and byproducts and improving NADPH availability, a record-high titer of optically pure (R)-1,3-BDO was achieved.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Hung Li, Nam Ngoc Pham, Claire R. Shen, Chin-Wei Chang, Yi Tu, Yi-Hao Chang, Jui Tu, Mai Thanh Thi Nguyen, Yu-Chen Hu
Summary: In this study, a combinatorial CRISPR interference (CRISPRi) library strategy was used to enhance cyanobacterial 2,3-butanediol (2,3-BDO) production by regulating specific genes. The results showed that inhibiting certain genes can increase 2,3-BDO synthesis, with fbp and pdh suppression having a more significant effect. This method opens up new possibilities for metabolic engineering of cyanobacteria.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Environmental Sciences
Rajendran Velmurugan, Aran Incharoensakdi
Summary: There is a growing demand for biofuels worldwide, and cyanobacteria have unique characteristics that make them a promising candidate for biofuel production. Metabolic engineering and engineering of photosynthetic apparatus play crucial roles in developing economically feasible processes.
Article
Energy & Fuels
Nitesh Kumar Mund, Yisong Liu, Shaolin Chen
Summary: Biofuels, such as biodiesel and alcohols, can be produced efficiently by microalgae and cyanobacteria through metabolic engineering and synthetic biology approaches, offering a sustainable alternative to fossil fuels. These microorganisms have the ability to convert CO2 and water into carbon-rich lipids, making them superior to traditional oil-seed crops. Cyanobacteria, in particular, have established genetic backgrounds and tolerance to foreign genes, making them promising hosts for biofuel synthesis from CO2, light, and water.
Article
Biotechnology & Applied Microbiology
Lili Sheng, Abubakar Madika, Matthew S. H. Lau, Ying Zhang, Nigel P. Minton
Summary: This study engineered the thermophile Parageobacillus thermoglucosidasius NCIMB 11955 to produce 3-hydroxybutanone and 2,3-butanediol, organic compounds with commercial applications. By minimizing by-product formation and addressing redox imbalance, the production of targeted metabolites was significantly improved. The highest titre of 2,3-butanediol produced in Parageobacillus and Geobacillus species to date, 15.6 g/L, was achieved in media supplemented with 5% glucose.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Nicolas Huber, Edgar Alberto Alcala-Orozco, Thomas Rexer, Udo Reichl, Steffen Klamt
Summary: Cell-free production systems are commonly used for synthesizing industrial chemicals and biopharmaceuticals. This study presents a model-based optimization framework for cell-free enzyme cascades, taking into account parameter uncertainties. The approach was exemplified using the synthesis of GDP-fucose, resulting in significant improvements in the process.
METABOLIC ENGINEERING
(2024)
